Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus

Abstract This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in t...

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Autores principales:	Marco Gerdol, Daniela Lucente, Francesco Buonocore, Elia Poerio, Giuseppe Scapigliati, Simonetta Mattiucci, Alberto Pallavicini, Roberta Cimmaruta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad64b1070fe44265ab097d244ba6a0b5
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spelling	oai:doaj.org-article:ad64b1070fe44265ab097d244ba6a0b52021-12-02T15:09:39ZMolecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus10.1038/s41598-019-42003-52045-2322https://doaj.org/article/ad64b1070fe44265ab097d244ba6a0b52019-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-42003-5https://doaj.org/toc/2045-2322Abstract This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in the exon 2 sequence of the highly expressed gene ChhaDAB has been observed, resulting in an estimate of 92 different variants translated in 87 different peptides from 54 analysed icefish individuals. A highly conservative estimate, based on a 95% sequence identity threshold clustering, translate this variability in 41 different peptide clusters belonging to four different clades and showing the signature of different kinds of selection. In stark contrast, the poorly expressed ChhaDBB gene displayed a very low level of molecular diversity within exon 2, in agreement with expectations for a nonclassical MHC class II β gene.Marco GerdolDaniela LucenteFrancesco BuonocoreElia PoerioGiuseppe ScapigliatiSimonetta MattiucciAlberto PallaviciniRoberta CimmarutaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marco Gerdol Daniela Lucente Francesco Buonocore Elia Poerio Giuseppe Scapigliati Simonetta Mattiucci Alberto Pallavicini Roberta Cimmaruta Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus
description	Abstract This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the application of targeted sequencing approach, a remarkable molecular diversity in the exon 2 sequence of the highly expressed gene ChhaDAB has been observed, resulting in an estimate of 92 different variants translated in 87 different peptides from 54 analysed icefish individuals. A highly conservative estimate, based on a 95% sequence identity threshold clustering, translate this variability in 41 different peptide clusters belonging to four different clades and showing the signature of different kinds of selection. In stark contrast, the poorly expressed ChhaDBB gene displayed a very low level of molecular diversity within exon 2, in agreement with expectations for a nonclassical MHC class II β gene.
format	article
author	Marco Gerdol Daniela Lucente Francesco Buonocore Elia Poerio Giuseppe Scapigliati Simonetta Mattiucci Alberto Pallavicini Roberta Cimmaruta
author_facet	Marco Gerdol Daniela Lucente Francesco Buonocore Elia Poerio Giuseppe Scapigliati Simonetta Mattiucci Alberto Pallavicini Roberta Cimmaruta
author_sort	Marco Gerdol
title	Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus
title_short	Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus
title_full	Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus
title_fullStr	Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus
title_full_unstemmed	Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus
title_sort	molecular and structural characterization of mhc class ii β genes reveals high diversity in the cold-adapted icefish chionodraco hamatus
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ad64b1070fe44265ab097d244ba6a0b5
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Molecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus

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