Molecular response mechanisms of silkworm (Bombyx mori L.) to the toxicity of 1-octyl-3-methylimidazole chloride based on transcriptome analysis of midguts and silk glands

In a previous study, silkworm larvae were used as a novel model to assess the biotoxicity of ILs, which showed that ILs could cause significant physiological and biochemical changes in midguts and silk glands of the larvae, and result in the death of larvae. In order to investigate the toxicity of 1...

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Autores principales:	Kun Gao, Mengting Yang, Bin Li, Runzhen Chen, Jingwei Dong, Qiaoqiao Liu, Zheng Gao, Xijie Guo, Xiangyuan Deng
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Bombyx mori 1-octyl-3-methylimidazole chloride Transcriptome analysis Cell apoptosis Histopathological observation Molecular mechanism Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/ad6628ace1cf42fb8e844367cf5d5cce
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spelling	oai:doaj.org-article:ad6628ace1cf42fb8e844367cf5d5cce2021-11-06T04:17:04ZMolecular response mechanisms of silkworm (Bombyx mori L.) to the toxicity of 1-octyl-3-methylimidazole chloride based on transcriptome analysis of midguts and silk glands0147-651310.1016/j.ecoenv.2021.112915https://doaj.org/article/ad6628ace1cf42fb8e844367cf5d5cce2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010277https://doaj.org/toc/0147-6513In a previous study, silkworm larvae were used as a novel model to assess the biotoxicity of ILs, which showed that ILs could cause significant physiological and biochemical changes in midguts and silk glands of the larvae, and result in the death of larvae. In order to investigate the toxicity of 1-octyl-3-methylimidazole chloride ([C8mim]Cl) to the larvae at molecular level, RNA-sequencing technology was used to construct transcriptomic profiles of midguts and silk glands in this work. Results showed that a lot of differentially expressed genes (DEGs) were effectively screened out through bioinformatics software based on the transcriptome data and reference genome. To give more detail, 5118 and 2211 DEGs (926 and 822 DEGs) were obtained in the midguts (silk glands) when the larvae were exposed to [C8mim]Cl for 6 and 12 h, respectively, relative to the controls. In addition, gene ontology (GO) analysis suggested that the DEGs could be divided into three categories (i.e., biological process, cellular component, and molecular function), and were involved in multiple organelle functions and complex biological processes. Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that the DEGs were enriched in a variety of pathways, such as signal transduction, apoptosis, glycolysis, peroxisome, autophagy, hippo signaling pathway, arginine and proline metabolism. Results of quantitative real-time PCR and histopathological observation indicated that molecular mechanism of the larvae against [C8mim]Cl toxicology may be attributed to cell apoptosis regulation via both the mitochondrial pathway and the death receptor-initiated pathway. Thus, these results provided useful data for exploring the toxicity of ILs to insects at molecular level.Kun GaoMengting YangBin LiRunzhen ChenJingwei DongQiaoqiao LiuZheng GaoXijie GuoXiangyuan DengElsevierarticleBombyx mori1-octyl-3-methylimidazole chlorideTranscriptome analysisCell apoptosisHistopathological observationMolecular mechanismEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112915- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bombyx mori 1-octyl-3-methylimidazole chloride Transcriptome analysis Cell apoptosis Histopathological observation Molecular mechanism Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Bombyx mori 1-octyl-3-methylimidazole chloride Transcriptome analysis Cell apoptosis Histopathological observation Molecular mechanism Environmental pollution TD172-193.5 Environmental sciences GE1-350 Kun Gao Mengting Yang Bin Li Runzhen Chen Jingwei Dong Qiaoqiao Liu Zheng Gao Xijie Guo Xiangyuan Deng Molecular response mechanisms of silkworm (Bombyx mori L.) to the toxicity of 1-octyl-3-methylimidazole chloride based on transcriptome analysis of midguts and silk glands
description	In a previous study, silkworm larvae were used as a novel model to assess the biotoxicity of ILs, which showed that ILs could cause significant physiological and biochemical changes in midguts and silk glands of the larvae, and result in the death of larvae. In order to investigate the toxicity of 1-octyl-3-methylimidazole chloride ([C8mim]Cl) to the larvae at molecular level, RNA-sequencing technology was used to construct transcriptomic profiles of midguts and silk glands in this work. Results showed that a lot of differentially expressed genes (DEGs) were effectively screened out through bioinformatics software based on the transcriptome data and reference genome. To give more detail, 5118 and 2211 DEGs (926 and 822 DEGs) were obtained in the midguts (silk glands) when the larvae were exposed to [C8mim]Cl for 6 and 12 h, respectively, relative to the controls. In addition, gene ontology (GO) analysis suggested that the DEGs could be divided into three categories (i.e., biological process, cellular component, and molecular function), and were involved in multiple organelle functions and complex biological processes. Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that the DEGs were enriched in a variety of pathways, such as signal transduction, apoptosis, glycolysis, peroxisome, autophagy, hippo signaling pathway, arginine and proline metabolism. Results of quantitative real-time PCR and histopathological observation indicated that molecular mechanism of the larvae against [C8mim]Cl toxicology may be attributed to cell apoptosis regulation via both the mitochondrial pathway and the death receptor-initiated pathway. Thus, these results provided useful data for exploring the toxicity of ILs to insects at molecular level.
format	article
author	Kun Gao Mengting Yang Bin Li Runzhen Chen Jingwei Dong Qiaoqiao Liu Zheng Gao Xijie Guo Xiangyuan Deng
author_facet	Kun Gao Mengting Yang Bin Li Runzhen Chen Jingwei Dong Qiaoqiao Liu Zheng Gao Xijie Guo Xiangyuan Deng
author_sort	Kun Gao
title	Molecular response mechanisms of silkworm (Bombyx mori L.) to the toxicity of 1-octyl-3-methylimidazole chloride based on transcriptome analysis of midguts and silk glands
title_short	Molecular response mechanisms of silkworm (Bombyx mori L.) to the toxicity of 1-octyl-3-methylimidazole chloride based on transcriptome analysis of midguts and silk glands
title_full	Molecular response mechanisms of silkworm (Bombyx mori L.) to the toxicity of 1-octyl-3-methylimidazole chloride based on transcriptome analysis of midguts and silk glands
title_fullStr	Molecular response mechanisms of silkworm (Bombyx mori L.) to the toxicity of 1-octyl-3-methylimidazole chloride based on transcriptome analysis of midguts and silk glands
title_full_unstemmed	Molecular response mechanisms of silkworm (Bombyx mori L.) to the toxicity of 1-octyl-3-methylimidazole chloride based on transcriptome analysis of midguts and silk glands
title_sort	molecular response mechanisms of silkworm (bombyx mori l.) to the toxicity of 1-octyl-3-methylimidazole chloride based on transcriptome analysis of midguts and silk glands
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/ad6628ace1cf42fb8e844367cf5d5cce
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Molecular response mechanisms of silkworm (Bombyx mori L.) to the toxicity of 1-octyl-3-methylimidazole chloride based on transcriptome analysis of midguts and silk glands

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