High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell

Long-read sequencing technologies facilitate efficient and high quality genome assembly. Here Michael et al. achieve a fast reference assembly for Arabidopsis thaliana KBS-Mac-74 accession using the handheld Oxford Nanopore MinION sequencer and consumer computing hardware, and demonstrate its useful...

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Autores principales: Todd P. Michael, Florian Jupe, Felix Bemm, S. Timothy Motley, Justin P. Sandoval, Christa Lanz, Olivier Loudet, Detlef Weigel, Joseph R. Ecker
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Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/ad6bc43df0404861ae63cd85f221e0b0
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spelling oai:doaj.org-article:ad6bc43df0404861ae63cd85f221e0b02021-12-02T14:40:09ZHigh contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell10.1038/s41467-018-03016-22041-1723https://doaj.org/article/ad6bc43df0404861ae63cd85f221e0b02018-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03016-2https://doaj.org/toc/2041-1723Long-read sequencing technologies facilitate efficient and high quality genome assembly. Here Michael et al. achieve a fast reference assembly for Arabidopsis thaliana KBS-Mac-74 accession using the handheld Oxford Nanopore MinION sequencer and consumer computing hardware, and demonstrate its usefulness in resolving complex structural variation.Todd P. MichaelFlorian JupeFelix BemmS. Timothy MotleyJustin P. SandovalChrista LanzOlivier LoudetDetlef WeigelJoseph R. EckerNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-8 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Todd P. Michael
Florian Jupe
Felix Bemm
S. Timothy Motley
Justin P. Sandoval
Christa Lanz
Olivier Loudet
Detlef Weigel
Joseph R. Ecker
High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell
description Long-read sequencing technologies facilitate efficient and high quality genome assembly. Here Michael et al. achieve a fast reference assembly for Arabidopsis thaliana KBS-Mac-74 accession using the handheld Oxford Nanopore MinION sequencer and consumer computing hardware, and demonstrate its usefulness in resolving complex structural variation.
format article
author Todd P. Michael
Florian Jupe
Felix Bemm
S. Timothy Motley
Justin P. Sandoval
Christa Lanz
Olivier Loudet
Detlef Weigel
Joseph R. Ecker
author_facet Todd P. Michael
Florian Jupe
Felix Bemm
S. Timothy Motley
Justin P. Sandoval
Christa Lanz
Olivier Loudet
Detlef Weigel
Joseph R. Ecker
author_sort Todd P. Michael
title High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell
title_short High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell
title_full High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell
title_fullStr High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell
title_full_unstemmed High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell
title_sort high contiguity arabidopsis thaliana genome assembly with a single nanopore flow cell
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/ad6bc43df0404861ae63cd85f221e0b0
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