Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions

Tubulointerstitial fibrosis leads to tubular basement membrane thickening and accumulation of interstitial extracellular matrix (ECM). Matrix metallopeptidase-2 (MMP-2) is involved in the breakdown of ECM. Chronic hypoxia often occurs in the kidney tissues of patients with chronic kidney disease. Ou...

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Autores principales:	Yu Wenmin, Ke Xiumei, Li Meiren, Ye Ping, Peng Jing, Li Huimin
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	proximal tubular cells hypoxia autophagy endocytosis matrix metalloproteinase-2 Medicine R
Acceso en línea:	https://doaj.org/article/ad6df08a0dde4a3cb8ee72315a7b60b5
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spelling	oai:doaj.org-article:ad6df08a0dde4a3cb8ee72315a7b60b52021-12-05T14:10:55ZDownregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions2391-546310.1515/med-2021-0341https://doaj.org/article/ad6df08a0dde4a3cb8ee72315a7b60b52021-09-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0341https://doaj.org/toc/2391-5463Tubulointerstitial fibrosis leads to tubular basement membrane thickening and accumulation of interstitial extracellular matrix (ECM). Matrix metallopeptidase-2 (MMP-2) is involved in the breakdown of ECM. Chronic hypoxia often occurs in the kidney tissues of patients with chronic kidney disease. Our previous study reported the effect of autophagy and endocytosis on MMP-2 activity in hypoxia-treated HK-2 cells. In this study, the relationship between the Ras-related protein Rab-7a (RAB7) and MMP-2 activity was further investigated. RAB7 overexpression decreased MMP-2 activity. In contrast, the results for RAB7 knockdown displayed the opposite pattern. Short hairpin RNA technology was used to knockdown Caveolin-1 (Cav-1) or Beclin-1 (Bec-1) in HK-2 cells. The two genes displayed differential effects on MMP-2 activity. Cav-1 and RAB7 interference increased MMP-2 activity. This study suggested that autophagy and endocytosis, RAB7, Cav-1, and Bec-1 may serve as potential mediators for altered MMP-2 activity.Yu WenminKe XiumeiLi MeirenYe PingPeng JingLi HuiminDe Gruyterarticleproximal tubular cellshypoxiaautophagyendocytosismatrix metalloproteinase-2MedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1428-1437 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	proximal tubular cells hypoxia autophagy endocytosis matrix metalloproteinase-2 Medicine R
spellingShingle	proximal tubular cells hypoxia autophagy endocytosis matrix metalloproteinase-2 Medicine R Yu Wenmin Ke Xiumei Li Meiren Ye Ping Peng Jing Li Huimin Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions
description	Tubulointerstitial fibrosis leads to tubular basement membrane thickening and accumulation of interstitial extracellular matrix (ECM). Matrix metallopeptidase-2 (MMP-2) is involved in the breakdown of ECM. Chronic hypoxia often occurs in the kidney tissues of patients with chronic kidney disease. Our previous study reported the effect of autophagy and endocytosis on MMP-2 activity in hypoxia-treated HK-2 cells. In this study, the relationship between the Ras-related protein Rab-7a (RAB7) and MMP-2 activity was further investigated. RAB7 overexpression decreased MMP-2 activity. In contrast, the results for RAB7 knockdown displayed the opposite pattern. Short hairpin RNA technology was used to knockdown Caveolin-1 (Cav-1) or Beclin-1 (Bec-1) in HK-2 cells. The two genes displayed differential effects on MMP-2 activity. Cav-1 and RAB7 interference increased MMP-2 activity. This study suggested that autophagy and endocytosis, RAB7, Cav-1, and Bec-1 may serve as potential mediators for altered MMP-2 activity.
format	article
author	Yu Wenmin Ke Xiumei Li Meiren Ye Ping Peng Jing Li Huimin
author_facet	Yu Wenmin Ke Xiumei Li Meiren Ye Ping Peng Jing Li Huimin
author_sort	Yu Wenmin
title	Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions
title_short	Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions
title_full	Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions
title_fullStr	Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions
title_full_unstemmed	Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions
title_sort	downregulation of rab7 and caveolin-1 increases mmp-2 activity in renal tubular epithelial cells under hypoxic conditions
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/ad6df08a0dde4a3cb8ee72315a7b60b5
work_keys_str_mv	AT yuwenmin downregulationofrab7andcaveolin1increasesmmp2activityinrenaltubularepithelialcellsunderhypoxicconditions AT kexiumei downregulationofrab7andcaveolin1increasesmmp2activityinrenaltubularepithelialcellsunderhypoxicconditions AT limeiren downregulationofrab7andcaveolin1increasesmmp2activityinrenaltubularepithelialcellsunderhypoxicconditions AT yeping downregulationofrab7andcaveolin1increasesmmp2activityinrenaltubularepithelialcellsunderhypoxicconditions AT pengjing downregulationofrab7andcaveolin1increasesmmp2activityinrenaltubularepithelialcellsunderhypoxicconditions AT lihuimin downregulationofrab7andcaveolin1increasesmmp2activityinrenaltubularepithelialcellsunderhypoxicconditions
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Downregulation of RAB7 and Caveolin-1 increases MMP-2 activity in renal tubular epithelial cells under hypoxic conditions

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