Osteoimmune Modulation and Guided Osteogenesis Promoted by Barrier Membranes Incorporated with S-Nitrosoglutathione (GSNO) and Mesenchymal Stem Cell-Derived Exosomes
Xin Wang,1,2,* Jun Ao,1,* Haiping Lu,1 Qingyu Zhao,1 Yaping Ma,1,2 Jun Zhang,1 Hao Ren,3 Yi Zhang2,4 1Department of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of China; 2Joint Orthopaedic Research Center of Zunyi Medical U...
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Dove Medical Press
2020
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oai:doaj.org-article:ad6edc17d296489d81575276b27a2f812021-12-02T09:14:38ZOsteoimmune Modulation and Guided Osteogenesis Promoted by Barrier Membranes Incorporated with S-Nitrosoglutathione (GSNO) and Mesenchymal Stem Cell-Derived Exosomes1178-2013https://doaj.org/article/ad6edc17d296489d81575276b27a2f812020-05-01T00:00:00Zhttps://www.dovepress.com/osteoimmune-modulation-and-guided-osteogenesis-promoted-by-barrier-mem-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xin Wang,1,2,* Jun Ao,1,* Haiping Lu,1 Qingyu Zhao,1 Yaping Ma,1,2 Jun Zhang,1 Hao Ren,3 Yi Zhang2,4 1Department of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of China; 2Joint Orthopaedic Research Center of Zunyi Medical University & University of Rochester Medical Center (JCMR-ZMU & URMC), Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of China; 3Shenzhen Institute for Innovation and Translational Medicine, Shenzhen International Biological Valley-Life Science Industrial Park, Shenzhen, Guangdong 518119, People’s Republic of China; 4Department of Hygiene Toxicology, School of Public Health, Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hao RenShenzhen Institute for Innovation and Translational Medicine Shenzhen International Biological Valley-Life Science Industrial Park, Shenzhen 518119, Guangdong, People’s Republic of ChinaTel +86 173 6972 4036Fax +86 755-8420 0009Email renhao0421@foxmail.comYi ZhangJoint Orthopaedic Research Center of Zunyi Medical University & University of Rochester Medical Center (JCMR-ZMU & URMC), Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of ChinaTel +86 182 7566 2009Fax +86 851-2860 9551Email zhangyizmr@163.comBackground: The use of polycaprolactone (PCL) for bone defects in a clinical setting is limited due to a lack of bioactivity. Exosomes derived from mesenchymal stem cells (MSCs) have an important immunoregulatory potential and together with S-nitrosoglutathione (GSNO) they possess therapeutic potential for bone regeneration.Materials and Methods: In this study, PCL was modified with GSNO and MSC-derived exosomes and the impact on macrophages and osteogenes is evaluated.Results: MSC-derived exosomes exhibited a cup-shaped morphology and were internalized by macrophages and human bone marrow-derived mesenchymal stromal cells (hBMSCs). The pattern of internalization of scaffold-immobilized exosomes was similar in RAW264.7 cells and hBMSCs after 4h and 24h of co-culture. Assessment of macrophage morphology under inflammatory conditions by scanning electronic microscopy (SEM) and confocal microscopy demonstrated macrophages were significantly elongated and expression of pro-inflammatory genes markedly decreased when co-cultured with PCL/PDA + GSNO + exosome scaffolds. Furthermore, this scaffold modification significantly enhanced osteogenic differentiation of hBMSCs.Discussion: This study demonstrated the possibility of using a GSNO- and exosome-based strategy to adapt barrier membrane scaffolds. PCL/PDA + GSNO + exosome scaffolds may serve as an important barrier membrane for osteogenesis and tissue regeneration.Keywords: polycaprolactone, mesenchymal stem cells, exosomes, S-nitrosoglutathione, osteogenesisWang XAo JLu HZhao QMa YZhang JRen HZhang YDove Medical Pressarticlepolycaprolactonemesenchymal stem cellsexosomess-nitrosoglutathioneosteogenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3483-3496 (2020) |
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polycaprolactone mesenchymal stem cells exosomes s-nitrosoglutathione osteogenesis Medicine (General) R5-920 |
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polycaprolactone mesenchymal stem cells exosomes s-nitrosoglutathione osteogenesis Medicine (General) R5-920 Wang X Ao J Lu H Zhao Q Ma Y Zhang J Ren H Zhang Y Osteoimmune Modulation and Guided Osteogenesis Promoted by Barrier Membranes Incorporated with S-Nitrosoglutathione (GSNO) and Mesenchymal Stem Cell-Derived Exosomes |
| description |
Xin Wang,1,2,* Jun Ao,1,* Haiping Lu,1 Qingyu Zhao,1 Yaping Ma,1,2 Jun Zhang,1 Hao Ren,3 Yi Zhang2,4 1Department of Orthopaedic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of China; 2Joint Orthopaedic Research Center of Zunyi Medical University & University of Rochester Medical Center (JCMR-ZMU & URMC), Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of China; 3Shenzhen Institute for Innovation and Translational Medicine, Shenzhen International Biological Valley-Life Science Industrial Park, Shenzhen, Guangdong 518119, People’s Republic of China; 4Department of Hygiene Toxicology, School of Public Health, Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hao RenShenzhen Institute for Innovation and Translational Medicine Shenzhen International Biological Valley-Life Science Industrial Park, Shenzhen 518119, Guangdong, People’s Republic of ChinaTel +86 173 6972 4036Fax +86 755-8420 0009Email renhao0421@foxmail.comYi ZhangJoint Orthopaedic Research Center of Zunyi Medical University & University of Rochester Medical Center (JCMR-ZMU & URMC), Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of ChinaTel +86 182 7566 2009Fax +86 851-2860 9551Email zhangyizmr@163.comBackground: The use of polycaprolactone (PCL) for bone defects in a clinical setting is limited due to a lack of bioactivity. Exosomes derived from mesenchymal stem cells (MSCs) have an important immunoregulatory potential and together with S-nitrosoglutathione (GSNO) they possess therapeutic potential for bone regeneration.Materials and Methods: In this study, PCL was modified with GSNO and MSC-derived exosomes and the impact on macrophages and osteogenes is evaluated.Results: MSC-derived exosomes exhibited a cup-shaped morphology and were internalized by macrophages and human bone marrow-derived mesenchymal stromal cells (hBMSCs). The pattern of internalization of scaffold-immobilized exosomes was similar in RAW264.7 cells and hBMSCs after 4h and 24h of co-culture. Assessment of macrophage morphology under inflammatory conditions by scanning electronic microscopy (SEM) and confocal microscopy demonstrated macrophages were significantly elongated and expression of pro-inflammatory genes markedly decreased when co-cultured with PCL/PDA + GSNO + exosome scaffolds. Furthermore, this scaffold modification significantly enhanced osteogenic differentiation of hBMSCs.Discussion: This study demonstrated the possibility of using a GSNO- and exosome-based strategy to adapt barrier membrane scaffolds. PCL/PDA + GSNO + exosome scaffolds may serve as an important barrier membrane for osteogenesis and tissue regeneration.Keywords: polycaprolactone, mesenchymal stem cells, exosomes, S-nitrosoglutathione, osteogenesis |
| format |
article |
| author |
Wang X Ao J Lu H Zhao Q Ma Y Zhang J Ren H Zhang Y |
| author_facet |
Wang X Ao J Lu H Zhao Q Ma Y Zhang J Ren H Zhang Y |
| author_sort |
Wang X |
| title |
Osteoimmune Modulation and Guided Osteogenesis Promoted by Barrier Membranes Incorporated with S-Nitrosoglutathione (GSNO) and Mesenchymal Stem Cell-Derived Exosomes |
| title_short |
Osteoimmune Modulation and Guided Osteogenesis Promoted by Barrier Membranes Incorporated with S-Nitrosoglutathione (GSNO) and Mesenchymal Stem Cell-Derived Exosomes |
| title_full |
Osteoimmune Modulation and Guided Osteogenesis Promoted by Barrier Membranes Incorporated with S-Nitrosoglutathione (GSNO) and Mesenchymal Stem Cell-Derived Exosomes |
| title_fullStr |
Osteoimmune Modulation and Guided Osteogenesis Promoted by Barrier Membranes Incorporated with S-Nitrosoglutathione (GSNO) and Mesenchymal Stem Cell-Derived Exosomes |
| title_full_unstemmed |
Osteoimmune Modulation and Guided Osteogenesis Promoted by Barrier Membranes Incorporated with S-Nitrosoglutathione (GSNO) and Mesenchymal Stem Cell-Derived Exosomes |
| title_sort |
osteoimmune modulation and guided osteogenesis promoted by barrier membranes incorporated with s-nitrosoglutathione (gsno) and mesenchymal stem cell-derived exosomes |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/ad6edc17d296489d81575276b27a2f81 |
| work_keys_str_mv |
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1718398204184100864 |