"Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.

The piRNA pathway protects genomes by silencing mobile elements. Despite advances in understanding the processing events that generate piRNAs for silencing, little is known about how primary transcripts are transported from their genomic clusters to their processing centers. Using a model of the Dro...

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Autores principales:	Cynthia Dennis, Vanessa Zanni, Emilie Brasset, Angeline Eymery, Liang Zhang, Rana Mteirek, Silke Jensen, Yikang S Rong, Chantal Vaury
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad70f319ff764079a81b3c768e515ad5
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spelling	oai:doaj.org-article:ad70f319ff764079a81b3c768e515ad52021-11-18T08:56:23Z"Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.1932-620310.1371/journal.pone.0072752https://doaj.org/article/ad70f319ff764079a81b3c768e515ad52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24039799/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The piRNA pathway protects genomes by silencing mobile elements. Despite advances in understanding the processing events that generate piRNAs for silencing, little is known about how primary transcripts are transported from their genomic clusters to their processing centers. Using a model of the Drosophila COM/flamenco locus in ovarian somatic cells, we identified a prominent nuclear structure called Dot COM, which is enriched in long transcripts from piRNA clusters but located far from their transcription sites. Remarkably, transcripts from multiple clusters accumulate at Dot COM, which is often juxtaposed with Yb-bodies, the cytoplasmic processing centers for cluster transcripts. Genetic evidence suggests that the accumulation of precursor transcripts at Dot COM represents one of the most upstream events in the piRNA pathway. Our results provide new insights into the initial steps of the piRNA pathway, and open up a new research area important for a complete understanding of this conserved pathway.Cynthia DennisVanessa ZanniEmilie BrassetAngeline EymeryLiang ZhangRana MteirekSilke JensenYikang S RongChantal VauryPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e72752 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cynthia Dennis Vanessa Zanni Emilie Brasset Angeline Eymery Liang Zhang Rana Mteirek Silke Jensen Yikang S Rong Chantal Vaury "Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.
description	The piRNA pathway protects genomes by silencing mobile elements. Despite advances in understanding the processing events that generate piRNAs for silencing, little is known about how primary transcripts are transported from their genomic clusters to their processing centers. Using a model of the Drosophila COM/flamenco locus in ovarian somatic cells, we identified a prominent nuclear structure called Dot COM, which is enriched in long transcripts from piRNA clusters but located far from their transcription sites. Remarkably, transcripts from multiple clusters accumulate at Dot COM, which is often juxtaposed with Yb-bodies, the cytoplasmic processing centers for cluster transcripts. Genetic evidence suggests that the accumulation of precursor transcripts at Dot COM represents one of the most upstream events in the piRNA pathway. Our results provide new insights into the initial steps of the piRNA pathway, and open up a new research area important for a complete understanding of this conserved pathway.
format	article
author	Cynthia Dennis Vanessa Zanni Emilie Brasset Angeline Eymery Liang Zhang Rana Mteirek Silke Jensen Yikang S Rong Chantal Vaury
author_facet	Cynthia Dennis Vanessa Zanni Emilie Brasset Angeline Eymery Liang Zhang Rana Mteirek Silke Jensen Yikang S Rong Chantal Vaury
author_sort	Cynthia Dennis
title	"Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.
title_short	"Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.
title_full	"Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.
title_fullStr	"Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.
title_full_unstemmed	"Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.
title_sort	"dot com", a nuclear transit center for the primary pirna pathway in drosophila.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ad70f319ff764079a81b3c768e515ad5
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"Dot COM", a nuclear transit center for the primary piRNA pathway in Drosophila.

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