Green tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats.

Our previous studies showed that an extract from Camellia sinenesis (green tea), which contains several polyphenols, attenuates nephrotoxicity caused by cyclosporine A (CsA). Since polyphenols are stimulators of mitochondrial biogenesis (MB), this study investigated whether stimulation of MB plays a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hasibur Rehman, Yasodha Krishnasamy, Khujista Haque, Ronald G Thurman, John J Lemasters, Rick G Schnellmann, Zhi Zhong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad71d200438b40a7ae923c0cdc9b1c11
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ad71d200438b40a7ae923c0cdc9b1c11
record_format	dspace
spelling	oai:doaj.org-article:ad71d200438b40a7ae923c0cdc9b1c112021-11-18T07:43:15ZGreen tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats.1932-620310.1371/journal.pone.0065029https://doaj.org/article/ad71d200438b40a7ae923c0cdc9b1c112014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23755172/?tool=EBIhttps://doaj.org/toc/1932-6203Our previous studies showed that an extract from Camellia sinenesis (green tea), which contains several polyphenols, attenuates nephrotoxicity caused by cyclosporine A (CsA). Since polyphenols are stimulators of mitochondrial biogenesis (MB), this study investigated whether stimulation of MB plays a role in green tea polyphenol protection against CsA renal toxicity. Rats were fed a powdered diet containing green tea polyphenolic extract (0.1%) starting 3 days prior to CsA treatment (25 mg/kg, i.g. daily for 3 weeks). CsA alone decreased renal nuclear DNA-encoded oxidative phosphorylation (OXPHOS) protein ATP synthase-β (AS-β) by 42%, mitochondrial DNA (mtDNA)-encoded OXPHOS protein NADH dehydrogenase-3 (ND3) by 87% and their associated mRNAs. Mitochondrial DNA copy number was also decreased by 78% by CsA. Immunohistochemical analysis showed decreased cytochrome c oxidase subunit IV (COX-IV), an OXPHOS protein, in tubular cells. Peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α, the master regulator of MB, and mitochondrial transcription factor-A (Tfam), the transcription factor that regulates mtDNA replication and transcription, were 42% and 90% lower, respectively, in the kidneys of CsA-treated than in untreated rats. These results indicate suppression of MB by chronic CsA treatment. Green tea polyphenols alone and following CsA increased AS-β, ND3, COX-IV, mtDNA copy number, PGC-1α mRNA and protein, decreased acetylated PGC-1α, and increased Tfam mRNA and protein. In association with suppressed MB, CsA increased serum creatinine, caused loss of brush border and dilatation of proximal tubules, tubular atrophy, vacuolization, apoptosis, calcification, and increased neutrophil gelatinase-associated lipocalin expression, leukocyte infiltration, and renal fibrosis. Green tea polyphenols markedly attenuated CsA-induced renal injury and improved renal function. Together, these results demonstrate that green tea polyphenols attenuate CsA-induced kidney injury, at least in part, through the stimulation of MB.Hasibur RehmanYasodha KrishnasamyKhujista HaqueRonald G ThurmanJohn J LemastersRick G SchnellmannZhi ZhongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65029 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hasibur Rehman Yasodha Krishnasamy Khujista Haque Ronald G Thurman John J Lemasters Rick G Schnellmann Zhi Zhong Green tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats.
description	Our previous studies showed that an extract from Camellia sinenesis (green tea), which contains several polyphenols, attenuates nephrotoxicity caused by cyclosporine A (CsA). Since polyphenols are stimulators of mitochondrial biogenesis (MB), this study investigated whether stimulation of MB plays a role in green tea polyphenol protection against CsA renal toxicity. Rats were fed a powdered diet containing green tea polyphenolic extract (0.1%) starting 3 days prior to CsA treatment (25 mg/kg, i.g. daily for 3 weeks). CsA alone decreased renal nuclear DNA-encoded oxidative phosphorylation (OXPHOS) protein ATP synthase-β (AS-β) by 42%, mitochondrial DNA (mtDNA)-encoded OXPHOS protein NADH dehydrogenase-3 (ND3) by 87% and their associated mRNAs. Mitochondrial DNA copy number was also decreased by 78% by CsA. Immunohistochemical analysis showed decreased cytochrome c oxidase subunit IV (COX-IV), an OXPHOS protein, in tubular cells. Peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α, the master regulator of MB, and mitochondrial transcription factor-A (Tfam), the transcription factor that regulates mtDNA replication and transcription, were 42% and 90% lower, respectively, in the kidneys of CsA-treated than in untreated rats. These results indicate suppression of MB by chronic CsA treatment. Green tea polyphenols alone and following CsA increased AS-β, ND3, COX-IV, mtDNA copy number, PGC-1α mRNA and protein, decreased acetylated PGC-1α, and increased Tfam mRNA and protein. In association with suppressed MB, CsA increased serum creatinine, caused loss of brush border and dilatation of proximal tubules, tubular atrophy, vacuolization, apoptosis, calcification, and increased neutrophil gelatinase-associated lipocalin expression, leukocyte infiltration, and renal fibrosis. Green tea polyphenols markedly attenuated CsA-induced renal injury and improved renal function. Together, these results demonstrate that green tea polyphenols attenuate CsA-induced kidney injury, at least in part, through the stimulation of MB.
format	article
author	Hasibur Rehman Yasodha Krishnasamy Khujista Haque Ronald G Thurman John J Lemasters Rick G Schnellmann Zhi Zhong
author_facet	Hasibur Rehman Yasodha Krishnasamy Khujista Haque Ronald G Thurman John J Lemasters Rick G Schnellmann Zhi Zhong
author_sort	Hasibur Rehman
title	Green tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats.
title_short	Green tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats.
title_full	Green tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats.
title_fullStr	Green tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats.
title_full_unstemmed	Green tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats.
title_sort	green tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ad71d200438b40a7ae923c0cdc9b1c11
work_keys_str_mv	AT hasiburrehman greenteapolyphenolsstimulatemitochondrialbiogenesisandimproverenalfunctionafterchroniccyclosporinatreatmentinrats AT yasodhakrishnasamy greenteapolyphenolsstimulatemitochondrialbiogenesisandimproverenalfunctionafterchroniccyclosporinatreatmentinrats AT khujistahaque greenteapolyphenolsstimulatemitochondrialbiogenesisandimproverenalfunctionafterchroniccyclosporinatreatmentinrats AT ronaldgthurman greenteapolyphenolsstimulatemitochondrialbiogenesisandimproverenalfunctionafterchroniccyclosporinatreatmentinrats AT johnjlemasters greenteapolyphenolsstimulatemitochondrialbiogenesisandimproverenalfunctionafterchroniccyclosporinatreatmentinrats AT rickgschnellmann greenteapolyphenolsstimulatemitochondrialbiogenesisandimproverenalfunctionafterchroniccyclosporinatreatmentinrats AT zhizhong greenteapolyphenolsstimulatemitochondrialbiogenesisandimproverenalfunctionafterchroniccyclosporinatreatmentinrats
_version_	1718423055147991040

Green tea polyphenols stimulate mitochondrial biogenesis and improve renal function after chronic cyclosporin a treatment in rats.

Ejemplares similares