Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide

Abstract A soluble melanin pigment produced by Streptomyces sp. ZL-24 was purified and named StrSM. The elemental analysis of StrSM showed it consists of carbon, hydrogen, and oxygen. The spectrum analysis, including ultraviolet–visible absorption spectrum, Fourier-transform infrared spectrum, and p...

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Autores principales:	Yumei Li, Zhengmao Ye, Peng Lu, Lingchao Lu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad738c885e794083a4caddeb1694eba7
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spelling	oai:doaj.org-article:ad738c885e794083a4caddeb1694eba72021-12-02T17:08:44ZPyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide10.1038/s41598-021-94598-32045-2322https://doaj.org/article/ad738c885e794083a4caddeb1694eba72021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94598-3https://doaj.org/toc/2045-2322Abstract A soluble melanin pigment produced by Streptomyces sp. ZL-24 was purified and named StrSM. The elemental analysis of StrSM showed it consists of carbon, hydrogen, and oxygen. The spectrum analysis, including ultraviolet–visible absorption spectrum, Fourier-transform infrared spectrum, and pyrolysis–gas chromatography–mass spectrometry, indicated that StrSM might be pyomelanin. High performance liquid chromatography and liquid chromatography–mass spectra analysis of intermediate metabolite showed the presence of homogentisic acid (HGA). Moreover, the enzyme 4-hydroxyphenylpyruvate dioxygenase, involved in HGA biosynthesis, showed high activity during melanin production. Subsequently, a tyrosinase gene (melC2) and hydroxyphenylpyruvate dioxygenase gene double mutant demonstrated StrSM is pyomelanin. In vitro bioactivity assay showed that StrSM had excellent protective capability against SH-SY5Y cell oxidative injury. To our knowledge, the results firstly provide comprehensive data on Streptomyces pyomelanin identification and a promising candidate compound to treat oxidative injury of neurocytes.Yumei LiZhengmao YePeng LuLingchao LuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yumei Li Zhengmao Ye Peng Lu Lingchao Lu Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide
description	Abstract A soluble melanin pigment produced by Streptomyces sp. ZL-24 was purified and named StrSM. The elemental analysis of StrSM showed it consists of carbon, hydrogen, and oxygen. The spectrum analysis, including ultraviolet–visible absorption spectrum, Fourier-transform infrared spectrum, and pyrolysis–gas chromatography–mass spectrometry, indicated that StrSM might be pyomelanin. High performance liquid chromatography and liquid chromatography–mass spectra analysis of intermediate metabolite showed the presence of homogentisic acid (HGA). Moreover, the enzyme 4-hydroxyphenylpyruvate dioxygenase, involved in HGA biosynthesis, showed high activity during melanin production. Subsequently, a tyrosinase gene (melC2) and hydroxyphenylpyruvate dioxygenase gene double mutant demonstrated StrSM is pyomelanin. In vitro bioactivity assay showed that StrSM had excellent protective capability against SH-SY5Y cell oxidative injury. To our knowledge, the results firstly provide comprehensive data on Streptomyces pyomelanin identification and a promising candidate compound to treat oxidative injury of neurocytes.
format	article
author	Yumei Li Zhengmao Ye Peng Lu Lingchao Lu
author_facet	Yumei Li Zhengmao Ye Peng Lu Lingchao Lu
author_sort	Yumei Li
title	Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide
title_short	Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide
title_full	Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide
title_fullStr	Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide
title_full_unstemmed	Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide
title_sort	pyomelanin produced by streptomyces sp. zl-24 and its protective effects against sh-sy5y cells injury induced by hydrogen peroxide
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ad738c885e794083a4caddeb1694eba7
work_keys_str_mv	AT yumeili pyomelaninproducedbystreptomycesspzl24anditsprotectiveeffectsagainstshsy5ycellsinjuryinducedbyhydrogenperoxide AT zhengmaoye pyomelaninproducedbystreptomycesspzl24anditsprotectiveeffectsagainstshsy5ycellsinjuryinducedbyhydrogenperoxide AT penglu pyomelaninproducedbystreptomycesspzl24anditsprotectiveeffectsagainstshsy5ycellsinjuryinducedbyhydrogenperoxide AT lingchaolu pyomelaninproducedbystreptomycesspzl24anditsprotectiveeffectsagainstshsy5ycellsinjuryinducedbyhydrogenperoxide
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Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide

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