Multifunctional 3D-Printed Magnetic Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering

Multifunctional and resistant 3D structures represent a great promise and a great challenge in bone tissue engineering. This study addresses this problem by employing polycaprolactone (PCL)-based scaffolds added with hydroxyapatite (HAp) and superparamagnetic iron oxide nanoparticles (SPION), able t...

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Autores principales:	Mauro Petretta, Alessandro Gambardella, Giovanna Desando, Carola Cavallo, Isabella Bartolotti, Tatiana Shelyakova, Vitaly Goranov, Marco Brucale, Valentin Alek Dediu, Milena Fini, Brunella Grigolo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polycaprolactone/hydroxyapatite scaffolds 3D additive manufacturing superparamagnetic nanoparticles nanocomposites tissue engineering Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/ad743703982d47d6b7dc26a7828fff37
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spelling	oai:doaj.org-article:ad743703982d47d6b7dc26a7828fff372021-11-11T18:48:27ZMultifunctional 3D-Printed Magnetic Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering10.3390/polym132138252073-4360https://doaj.org/article/ad743703982d47d6b7dc26a7828fff372021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3825https://doaj.org/toc/2073-4360Multifunctional and resistant 3D structures represent a great promise and a great challenge in bone tissue engineering. This study addresses this problem by employing polycaprolactone (PCL)-based scaffolds added with hydroxyapatite (HAp) and superparamagnetic iron oxide nanoparticles (SPION), able to drive on demand the necessary cells and other bioagents for a high healing efficiency. PCL-HAp-SPION scaffolds with different concentrations of the superparamagnetic component were developed through the 3D-printing technology and the specific topographical features were detected by Atomic Force and Magnetic Force Microscopy (AFM-MFM). AFM-MFM measurements confirmed a homogenous distribution of HAp and SPION throughout the surface. The magnetically assisted seeding of cells in the scaffold resulted most efficient for the 1% SPION concentration, providing good cell entrapment and adhesion rates. Mesenchymal Stromal Cells (MSCs) seeded onto PCL-HAp-1% SPION showed a good cell proliferation and intrinsic osteogenic potential, indicating no toxic effects of the employed scaffold materials. The performed characterizations and the collected set of data point on the inherent osteogenic potential of the newly developed PCL-HAp-1% SPION scaffolds, endorsing them towards next steps of in vitro and in vivo studies and validations.Mauro PetrettaAlessandro GambardellaGiovanna DesandoCarola CavalloIsabella BartolottiTatiana ShelyakovaVitaly GoranovMarco BrucaleValentin Alek DediuMilena FiniBrunella GrigoloMDPI AGarticlepolycaprolactone/hydroxyapatite scaffolds3D additive manufacturingsuperparamagnetic nanoparticlesnanocompositestissue engineeringOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3825, p 3825 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polycaprolactone/hydroxyapatite scaffolds 3D additive manufacturing superparamagnetic nanoparticles nanocomposites tissue engineering Organic chemistry QD241-441
spellingShingle	polycaprolactone/hydroxyapatite scaffolds 3D additive manufacturing superparamagnetic nanoparticles nanocomposites tissue engineering Organic chemistry QD241-441 Mauro Petretta Alessandro Gambardella Giovanna Desando Carola Cavallo Isabella Bartolotti Tatiana Shelyakova Vitaly Goranov Marco Brucale Valentin Alek Dediu Milena Fini Brunella Grigolo Multifunctional 3D-Printed Magnetic Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering
description	Multifunctional and resistant 3D structures represent a great promise and a great challenge in bone tissue engineering. This study addresses this problem by employing polycaprolactone (PCL)-based scaffolds added with hydroxyapatite (HAp) and superparamagnetic iron oxide nanoparticles (SPION), able to drive on demand the necessary cells and other bioagents for a high healing efficiency. PCL-HAp-SPION scaffolds with different concentrations of the superparamagnetic component were developed through the 3D-printing technology and the specific topographical features were detected by Atomic Force and Magnetic Force Microscopy (AFM-MFM). AFM-MFM measurements confirmed a homogenous distribution of HAp and SPION throughout the surface. The magnetically assisted seeding of cells in the scaffold resulted most efficient for the 1% SPION concentration, providing good cell entrapment and adhesion rates. Mesenchymal Stromal Cells (MSCs) seeded onto PCL-HAp-1% SPION showed a good cell proliferation and intrinsic osteogenic potential, indicating no toxic effects of the employed scaffold materials. The performed characterizations and the collected set of data point on the inherent osteogenic potential of the newly developed PCL-HAp-1% SPION scaffolds, endorsing them towards next steps of in vitro and in vivo studies and validations.
format	article
author	Mauro Petretta Alessandro Gambardella Giovanna Desando Carola Cavallo Isabella Bartolotti Tatiana Shelyakova Vitaly Goranov Marco Brucale Valentin Alek Dediu Milena Fini Brunella Grigolo
author_facet	Mauro Petretta Alessandro Gambardella Giovanna Desando Carola Cavallo Isabella Bartolotti Tatiana Shelyakova Vitaly Goranov Marco Brucale Valentin Alek Dediu Milena Fini Brunella Grigolo
author_sort	Mauro Petretta
title	Multifunctional 3D-Printed Magnetic Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering
title_short	Multifunctional 3D-Printed Magnetic Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering
title_full	Multifunctional 3D-Printed Magnetic Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering
title_fullStr	Multifunctional 3D-Printed Magnetic Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering
title_full_unstemmed	Multifunctional 3D-Printed Magnetic Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering
title_sort	multifunctional 3d-printed magnetic polycaprolactone/hydroxyapatite scaffolds for bone tissue engineering
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ad743703982d47d6b7dc26a7828fff37
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Multifunctional 3D-Printed Magnetic Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering

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