Chromatographic Determination of Total Selenium in Biofortified Allium sp. following Piazselenol Formation and Micro-Solid-Phase Extraction

Herein, a method based on selective piazselenol formation is applied for total selenium determination in biofortified Allium species. Piazselenol is formed by reacting Se(IV) with an aromatic diamine, namely 4-nitro-1,2-phenylenediamine, in acidic medium. Samples were digested in...

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Autores principales:	Bogdan M. Bosca, Augustin C. Mot
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	selenium <i>Allium</i> piazselenol micro-solid-phase extraction Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/ad7d94e9df6740be85269ea07e601ad0
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spelling	oai:doaj.org-article:ad7d94e9df6740be85269ea07e601ad02021-11-11T18:40:03ZChromatographic Determination of Total Selenium in Biofortified <i>Allium</i> <i>sp.</i> following Piazselenol Formation and Micro-Solid-Phase Extraction10.3390/molecules262167301420-3049https://doaj.org/article/ad7d94e9df6740be85269ea07e601ad02021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6730https://doaj.org/toc/1420-3049Herein, a method based on selective piazselenol formation is applied for total selenium determination in biofortified <i>Allium</i> species. Piazselenol is formed by reacting Se(IV) with an aromatic diamine, namely 4-nitro-1,2-phenylenediamine, in acidic medium. Samples were digested in a nitric acid/hydrogen peroxide open system, followed by selenate reduction in hydrochloric acid. Reaction conditions were optimized in terms of pH, temperature, reaction time, and other auxiliary reagents for interference removal, namely, EDTA and hydroxylamine. For the extraction of the selectively formed 4-nitro-piazselenol, micro-solid-phase extraction (μSPE) was applied, and the analysis and detection of the corresponding complex was performed by HPLC coupled with DAD. An external standard calibration curve was developed (R<sup>2</sup> = 0.9994) with good sensitivity, and was used to calculate the total selenium content from several <i>Allium</i> plants material, with good intermediate precision (RSD% < 16%). The accuracy of the method was evaluated using both, a comparison with an accepted reference method from our previously published data, as well as three certified reference material with recoveries between 84–126%. The limit of detection was determined to be 0.35 μg/g (in solids) and 1.1 μg/L (in solution), while the limit of quantification was 1.07 μg/g and 3.4 μg/L (in solution). Using the proposed method, selenium content can be quickly and accurately determined in several types of samples. In addition, this study present experimental conditions for overcoming the interferences that might be encountered in selenium determination using piazselenol.Bogdan M. BoscaAugustin C. MotMDPI AGarticleselenium<i>Allium</i>piazselenolmicro-solid-phase extractionOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6730, p 6730 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	selenium <i>Allium</i> piazselenol micro-solid-phase extraction Organic chemistry QD241-441
spellingShingle	selenium <i>Allium</i> piazselenol micro-solid-phase extraction Organic chemistry QD241-441 Bogdan M. Bosca Augustin C. Mot Chromatographic Determination of Total Selenium in Biofortified <i>Allium</i> <i>sp.</i> following Piazselenol Formation and Micro-Solid-Phase Extraction
description	Herein, a method based on selective piazselenol formation is applied for total selenium determination in biofortified <i>Allium</i> species. Piazselenol is formed by reacting Se(IV) with an aromatic diamine, namely 4-nitro-1,2-phenylenediamine, in acidic medium. Samples were digested in a nitric acid/hydrogen peroxide open system, followed by selenate reduction in hydrochloric acid. Reaction conditions were optimized in terms of pH, temperature, reaction time, and other auxiliary reagents for interference removal, namely, EDTA and hydroxylamine. For the extraction of the selectively formed 4-nitro-piazselenol, micro-solid-phase extraction (μSPE) was applied, and the analysis and detection of the corresponding complex was performed by HPLC coupled with DAD. An external standard calibration curve was developed (R<sup>2</sup> = 0.9994) with good sensitivity, and was used to calculate the total selenium content from several <i>Allium</i> plants material, with good intermediate precision (RSD% < 16%). The accuracy of the method was evaluated using both, a comparison with an accepted reference method from our previously published data, as well as three certified reference material with recoveries between 84–126%. The limit of detection was determined to be 0.35 μg/g (in solids) and 1.1 μg/L (in solution), while the limit of quantification was 1.07 μg/g and 3.4 μg/L (in solution). Using the proposed method, selenium content can be quickly and accurately determined in several types of samples. In addition, this study present experimental conditions for overcoming the interferences that might be encountered in selenium determination using piazselenol.
format	article
author	Bogdan M. Bosca Augustin C. Mot
author_facet	Bogdan M. Bosca Augustin C. Mot
author_sort	Bogdan M. Bosca
title	Chromatographic Determination of Total Selenium in Biofortified <i>Allium</i> <i>sp.</i> following Piazselenol Formation and Micro-Solid-Phase Extraction
title_short	Chromatographic Determination of Total Selenium in Biofortified <i>Allium</i> <i>sp.</i> following Piazselenol Formation and Micro-Solid-Phase Extraction
title_full	Chromatographic Determination of Total Selenium in Biofortified <i>Allium</i> <i>sp.</i> following Piazselenol Formation and Micro-Solid-Phase Extraction
title_fullStr	Chromatographic Determination of Total Selenium in Biofortified <i>Allium</i> <i>sp.</i> following Piazselenol Formation and Micro-Solid-Phase Extraction
title_full_unstemmed	Chromatographic Determination of Total Selenium in Biofortified <i>Allium</i> <i>sp.</i> following Piazselenol Formation and Micro-Solid-Phase Extraction
title_sort	chromatographic determination of total selenium in biofortified <i>allium</i> <i>sp.</i> following piazselenol formation and micro-solid-phase extraction
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ad7d94e9df6740be85269ea07e601ad0
work_keys_str_mv	AT bogdanmbosca chromatographicdeterminationoftotalseleniuminbiofortifiedialliumiispifollowingpiazselenolformationandmicrosolidphaseextraction AT augustincmot chromatographicdeterminationoftotalseleniuminbiofortifiedialliumiispifollowingpiazselenolformationandmicrosolidphaseextraction
_version_	1718431780107714560

Chromatographic Determination of Total Selenium in Biofortified <i>Allium</i> <i>sp.</i> following Piazselenol Formation and Micro-Solid-Phase Extraction

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