Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity

Abstract MCM7, a subunit of mini-chromosome maintenance proteins (MCM) complex, plays an important role in initiating DNA replication during the G1 phase and extending DNA strands during the S phase. Here, we demonstrated that MCM7 is not only sustained but maintains association with chromatin durin...

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Autores principales:	Dianpeng Zheng, Sichao Ye, Xiuyun Wang, Yongjun Zhang, Daoyu Yan, Xiangsheng Cai, Weihong Gao, Hongbo Shan, Yang Gao, Juanjuan Chen, Zhiming Hu, Hongwei Li, Jinlong Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ad7ef13dadd44128ad853042f16fc5cc
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spelling	oai:doaj.org-article:ad7ef13dadd44128ad853042f16fc5cc2021-12-02T15:05:38ZPre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity10.1038/s41598-017-03148-32045-2322https://doaj.org/article/ad7ef13dadd44128ad853042f16fc5cc2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03148-3https://doaj.org/toc/2045-2322Abstract MCM7, a subunit of mini-chromosome maintenance proteins (MCM) complex, plays an important role in initiating DNA replication during the G1 phase and extending DNA strands during the S phase. Here, we demonstrated that MCM7 is not only sustained but maintains association with chromatin during M phase. Remarkably, MCM7 siRNA can accelerate mitotic exit. MCM7 depletion leads to CDK1 inactivation and promotes subsequent cohesin/RAD21 cleavage, which eventually leads to sister chromatin segregation. Moreover, MCM7 is co-localized with tubulin in the mitotic cells and MCM7 depletion results in aberrant mitosis. Our results indicate that MCM7 may exert certain functions on spindle formation to prevent cytokinesis during early mitosis by regulating CDK1 activity.Dianpeng ZhengSichao YeXiuyun WangYongjun ZhangDaoyu YanXiangsheng CaiWeihong GaoHongbo ShanYang GaoJuanjuan ChenZhiming HuHongwei LiJinlong LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dianpeng Zheng Sichao Ye Xiuyun Wang Yongjun Zhang Daoyu Yan Xiangsheng Cai Weihong Gao Hongbo Shan Yang Gao Juanjuan Chen Zhiming Hu Hongwei Li Jinlong Li Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity
description	Abstract MCM7, a subunit of mini-chromosome maintenance proteins (MCM) complex, plays an important role in initiating DNA replication during the G1 phase and extending DNA strands during the S phase. Here, we demonstrated that MCM7 is not only sustained but maintains association with chromatin during M phase. Remarkably, MCM7 siRNA can accelerate mitotic exit. MCM7 depletion leads to CDK1 inactivation and promotes subsequent cohesin/RAD21 cleavage, which eventually leads to sister chromatin segregation. Moreover, MCM7 is co-localized with tubulin in the mitotic cells and MCM7 depletion results in aberrant mitosis. Our results indicate that MCM7 may exert certain functions on spindle formation to prevent cytokinesis during early mitosis by regulating CDK1 activity.
format	article
author	Dianpeng Zheng Sichao Ye Xiuyun Wang Yongjun Zhang Daoyu Yan Xiangsheng Cai Weihong Gao Hongbo Shan Yang Gao Juanjuan Chen Zhiming Hu Hongwei Li Jinlong Li
author_facet	Dianpeng Zheng Sichao Ye Xiuyun Wang Yongjun Zhang Daoyu Yan Xiangsheng Cai Weihong Gao Hongbo Shan Yang Gao Juanjuan Chen Zhiming Hu Hongwei Li Jinlong Li
author_sort	Dianpeng Zheng
title	Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity
title_short	Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity
title_full	Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity
title_fullStr	Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity
title_full_unstemmed	Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity
title_sort	pre-rc protein mcm7 depletion promotes mitotic exit by inhibiting cdk1 activity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ad7ef13dadd44128ad853042f16fc5cc
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Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity

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