Different modes of retrovirus restriction by human APOBEC3A and APOBEC3G in vivo.

The apolipoprotein B editing complex 3 (A3) cytidine deaminases are among the most highly evolutionarily selected retroviral restriction factors, both in terms of gene copy number and sequence diversity. Primate genomes encode seven A3 genes, and while A3F and 3G are widely recognized as important i...

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Autores principales:	Spyridon Stavrou, Daniel Crawford, Kristin Blouch, Edward P Browne, Rahul M Kohli, Susan R Ross
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ad7fe0281ae347bf8818a89272d92380
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spelling	oai:doaj.org-article:ad7fe0281ae347bf8818a89272d923802021-11-18T06:06:34ZDifferent modes of retrovirus restriction by human APOBEC3A and APOBEC3G in vivo.1553-73661553-737410.1371/journal.ppat.1004145https://doaj.org/article/ad7fe0281ae347bf8818a89272d923802014-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24851906/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The apolipoprotein B editing complex 3 (A3) cytidine deaminases are among the most highly evolutionarily selected retroviral restriction factors, both in terms of gene copy number and sequence diversity. Primate genomes encode seven A3 genes, and while A3F and 3G are widely recognized as important in the restriction of HIV, the role of the other genes, particularly A3A, is not as clear. Indeed, since human cells can express multiple A3 genes, and because of the lack of an experimentally tractable model, it is difficult to dissect the individual contribution of each gene to virus restriction in vivo. To overcome this problem, we generated human A3A and A3G transgenic mice on a mouse A3 knockout background. Using these mice, we demonstrate that both A3A and A3G restrict infection by murine retroviruses but by different mechanisms: A3G was packaged into virions and caused extensive deamination of the retrovirus genomes while A3A was not packaged and instead restricted infection when expressed in target cells. Additionally, we show that a murine leukemia virus engineered to express HIV Vif overcame the A3G-mediated restriction, thereby creating a novel model for studying the interaction between these proteins. We have thus developed an in vivo system for understanding how human A3 proteins use different modes of restriction, as well as a means for testing therapies that disrupt HIV Vif-A3G interactions.Spyridon StavrouDaniel CrawfordKristin BlouchEdward P BrowneRahul M KohliSusan R RossPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 10, Iss 5, p e1004145 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Spyridon Stavrou Daniel Crawford Kristin Blouch Edward P Browne Rahul M Kohli Susan R Ross Different modes of retrovirus restriction by human APOBEC3A and APOBEC3G in vivo.
description	The apolipoprotein B editing complex 3 (A3) cytidine deaminases are among the most highly evolutionarily selected retroviral restriction factors, both in terms of gene copy number and sequence diversity. Primate genomes encode seven A3 genes, and while A3F and 3G are widely recognized as important in the restriction of HIV, the role of the other genes, particularly A3A, is not as clear. Indeed, since human cells can express multiple A3 genes, and because of the lack of an experimentally tractable model, it is difficult to dissect the individual contribution of each gene to virus restriction in vivo. To overcome this problem, we generated human A3A and A3G transgenic mice on a mouse A3 knockout background. Using these mice, we demonstrate that both A3A and A3G restrict infection by murine retroviruses but by different mechanisms: A3G was packaged into virions and caused extensive deamination of the retrovirus genomes while A3A was not packaged and instead restricted infection when expressed in target cells. Additionally, we show that a murine leukemia virus engineered to express HIV Vif overcame the A3G-mediated restriction, thereby creating a novel model for studying the interaction between these proteins. We have thus developed an in vivo system for understanding how human A3 proteins use different modes of restriction, as well as a means for testing therapies that disrupt HIV Vif-A3G interactions.
format	article
author	Spyridon Stavrou Daniel Crawford Kristin Blouch Edward P Browne Rahul M Kohli Susan R Ross
author_facet	Spyridon Stavrou Daniel Crawford Kristin Blouch Edward P Browne Rahul M Kohli Susan R Ross
author_sort	Spyridon Stavrou
title	Different modes of retrovirus restriction by human APOBEC3A and APOBEC3G in vivo.
title_short	Different modes of retrovirus restriction by human APOBEC3A and APOBEC3G in vivo.
title_full	Different modes of retrovirus restriction by human APOBEC3A and APOBEC3G in vivo.
title_fullStr	Different modes of retrovirus restriction by human APOBEC3A and APOBEC3G in vivo.
title_full_unstemmed	Different modes of retrovirus restriction by human APOBEC3A and APOBEC3G in vivo.
title_sort	different modes of retrovirus restriction by human apobec3a and apobec3g in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ad7fe0281ae347bf8818a89272d92380
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Different modes of retrovirus restriction by human APOBEC3A and APOBEC3G in vivo.

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