Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods
Pathway optimization and chassis engineering are usually carried out in a step-wise and trial-and-error manner. Here the authors present ’SCRaMbLE-in’ that combines in-vitro pathway rapid prototyping with in-vivo genome integration and optimization.
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Nature Portfolio
2018
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oai:doaj.org-article:ad81529cd72f4cf5b421bbb08fbc1ca62021-12-02T16:49:54ZRapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods10.1038/s41467-018-04254-02041-1723https://doaj.org/article/ad81529cd72f4cf5b421bbb08fbc1ca62018-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04254-0https://doaj.org/toc/2041-1723Pathway optimization and chassis engineering are usually carried out in a step-wise and trial-and-error manner. Here the authors present ’SCRaMbLE-in’ that combines in-vitro pathway rapid prototyping with in-vivo genome integration and optimization.Wei LiuZhouqing LuoYun WangNhan T. PhamLaura TuckIrene Pérez-PiLongying LiuYue ShenChris FrenchManfred AuerJon Marles-WrightJunbiao DaiYizhi CaiNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018) |
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Science Q Wei Liu Zhouqing Luo Yun Wang Nhan T. Pham Laura Tuck Irene Pérez-Pi Longying Liu Yue Shen Chris French Manfred Auer Jon Marles-Wright Junbiao Dai Yizhi Cai Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods |
description |
Pathway optimization and chassis engineering are usually carried out in a step-wise and trial-and-error manner. Here the authors present ’SCRaMbLE-in’ that combines in-vitro pathway rapid prototyping with in-vivo genome integration and optimization. |
format |
article |
author |
Wei Liu Zhouqing Luo Yun Wang Nhan T. Pham Laura Tuck Irene Pérez-Pi Longying Liu Yue Shen Chris French Manfred Auer Jon Marles-Wright Junbiao Dai Yizhi Cai |
author_facet |
Wei Liu Zhouqing Luo Yun Wang Nhan T. Pham Laura Tuck Irene Pérez-Pi Longying Liu Yue Shen Chris French Manfred Auer Jon Marles-Wright Junbiao Dai Yizhi Cai |
author_sort |
Wei Liu |
title |
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods |
title_short |
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods |
title_full |
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods |
title_fullStr |
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods |
title_full_unstemmed |
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods |
title_sort |
rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome scramble-in methods |
publisher |
Nature Portfolio |
publishDate |
2018 |
url |
https://doaj.org/article/ad81529cd72f4cf5b421bbb08fbc1ca6 |
work_keys_str_mv |
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1718383178242064384 |