Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods

Pathway optimization and chassis engineering are usually carried out in a step-wise and trial-and-error manner. Here the authors present ’SCRaMbLE-in’ that combines in-vitro pathway rapid prototyping with in-vivo genome integration and optimization.

Guardado en:
Detalles Bibliográficos
Autores principales: Wei Liu, Zhouqing Luo, Yun Wang, Nhan T. Pham, Laura Tuck, Irene Pérez-Pi, Longying Liu, Yue Shen, Chris French, Manfred Auer, Jon Marles-Wright, Junbiao Dai, Yizhi Cai
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
Q
Acceso en línea:https://doaj.org/article/ad81529cd72f4cf5b421bbb08fbc1ca6
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:ad81529cd72f4cf5b421bbb08fbc1ca6
record_format dspace
spelling oai:doaj.org-article:ad81529cd72f4cf5b421bbb08fbc1ca62021-12-02T16:49:54ZRapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods10.1038/s41467-018-04254-02041-1723https://doaj.org/article/ad81529cd72f4cf5b421bbb08fbc1ca62018-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04254-0https://doaj.org/toc/2041-1723Pathway optimization and chassis engineering are usually carried out in a step-wise and trial-and-error manner. Here the authors present ’SCRaMbLE-in’ that combines in-vitro pathway rapid prototyping with in-vivo genome integration and optimization.Wei LiuZhouqing LuoYun WangNhan T. PhamLaura TuckIrene Pérez-PiLongying LiuYue ShenChris FrenchManfred AuerJon Marles-WrightJunbiao DaiYizhi CaiNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Wei Liu
Zhouqing Luo
Yun Wang
Nhan T. Pham
Laura Tuck
Irene Pérez-Pi
Longying Liu
Yue Shen
Chris French
Manfred Auer
Jon Marles-Wright
Junbiao Dai
Yizhi Cai
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods
description Pathway optimization and chassis engineering are usually carried out in a step-wise and trial-and-error manner. Here the authors present ’SCRaMbLE-in’ that combines in-vitro pathway rapid prototyping with in-vivo genome integration and optimization.
format article
author Wei Liu
Zhouqing Luo
Yun Wang
Nhan T. Pham
Laura Tuck
Irene Pérez-Pi
Longying Liu
Yue Shen
Chris French
Manfred Auer
Jon Marles-Wright
Junbiao Dai
Yizhi Cai
author_facet Wei Liu
Zhouqing Luo
Yun Wang
Nhan T. Pham
Laura Tuck
Irene Pérez-Pi
Longying Liu
Yue Shen
Chris French
Manfred Auer
Jon Marles-Wright
Junbiao Dai
Yizhi Cai
author_sort Wei Liu
title Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods
title_short Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods
title_full Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods
title_fullStr Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods
title_full_unstemmed Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods
title_sort rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome scramble-in methods
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/ad81529cd72f4cf5b421bbb08fbc1ca6
work_keys_str_mv AT weiliu rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT zhouqingluo rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT yunwang rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT nhantpham rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT lauratuck rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT ireneperezpi rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT longyingliu rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT yueshen rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT chrisfrench rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT manfredauer rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT jonmarleswright rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT junbiaodai rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
AT yizhicai rapidpathwayprototypingandengineeringusinginvitroandinvivosyntheticgenomescrambleinmethods
_version_ 1718383178242064384