Effect of a Fiber D-Limonene-Enriched Food Supplement on Intestinal Microbiota and Metabolic Parameters of Mice on a High-Fat Diet

Several studies showed that D-Limonene can improve metabolic parameters of obese mice via various mechanisms, including intestinal microbiota modulation. Nevertheless, its effective doses often overcome the acceptable daily intake, rising concerns about toxicity. In this study we administered to C57...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maria Chiara Valerii, Silvia Turroni, Carla Ferreri, Michela Zaro, Anna Sansone, Alessandro Dalpiaz, Giada Botti, Luca Ferraro, Renato Spigarelli, Irene Bellocchio, Federica D’Amico, Enzo Spisni
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	D-Limonene high fat diet (HFD) intestinal microbiota obesity Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/ad844570d81347adbcf8478c2e2f06f2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ad844570d81347adbcf8478c2e2f06f2
record_format	dspace
spelling	oai:doaj.org-article:ad844570d81347adbcf8478c2e2f06f22021-11-25T18:40:23ZEffect of a Fiber D-Limonene-Enriched Food Supplement on Intestinal Microbiota and Metabolic Parameters of Mice on a High-Fat Diet10.3390/pharmaceutics131117531999-4923https://doaj.org/article/ad844570d81347adbcf8478c2e2f06f22021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1753https://doaj.org/toc/1999-4923Several studies showed that D-Limonene can improve metabolic parameters of obese mice via various mechanisms, including intestinal microbiota modulation. Nevertheless, its effective doses often overcome the acceptable daily intake, rising concerns about toxicity. In this study we administered to C57BL/6 mice for 84 days a food supplement based on D-Limonene, adsorbed on dietary fibers (FLS), not able to reach the bloodstream, to counteract the metabolic effects of a high-fat diet (HFD). Results showed that daily administration of D-Limonene (30 and 60 mg/kg body weight) for 84 days decreased the weight gain of HFD mice. After 84 days we observed a statistically significant difference in weight gain in the group of mice receiving the higher dose of FLS compared to HFD mice (35.24 ± 4.56 g vs. 40.79 ± 3.28 g, <i>p</i> < 0.05). Moreover, FLS at both doses tested was capable of lowering triglyceridemia and also fasting glycemia at the higher dose. Some insights on the relevant fatty acid changes in hepatic tissues were obtained, highlighting the increased polyunsaturated fatty acid (PUFA) levels even at the lowest dose. FLS was also able to positively modulate the gut microbiota and prevent HFD-associated liver steatosis in a dose-dependent manner. These results demonstrate that FLS at these doses can be considered non-toxic and could be an effective tool to counteract diet-induced obesity and ameliorate metabolic profile in mice.Maria Chiara ValeriiSilvia TurroniCarla FerreriMichela ZaroAnna SansoneAlessandro DalpiazGiada BottiLuca FerraroRenato SpigarelliIrene BellocchioFederica D’AmicoEnzo SpisniMDPI AGarticleD-Limonenehigh fat diet (HFD)intestinal microbiotaobesityPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1753, p 1753 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	D-Limonene high fat diet (HFD) intestinal microbiota obesity Pharmacy and materia medica RS1-441
spellingShingle	D-Limonene high fat diet (HFD) intestinal microbiota obesity Pharmacy and materia medica RS1-441 Maria Chiara Valerii Silvia Turroni Carla Ferreri Michela Zaro Anna Sansone Alessandro Dalpiaz Giada Botti Luca Ferraro Renato Spigarelli Irene Bellocchio Federica D’Amico Enzo Spisni Effect of a Fiber D-Limonene-Enriched Food Supplement on Intestinal Microbiota and Metabolic Parameters of Mice on a High-Fat Diet
description	Several studies showed that D-Limonene can improve metabolic parameters of obese mice via various mechanisms, including intestinal microbiota modulation. Nevertheless, its effective doses often overcome the acceptable daily intake, rising concerns about toxicity. In this study we administered to C57BL/6 mice for 84 days a food supplement based on D-Limonene, adsorbed on dietary fibers (FLS), not able to reach the bloodstream, to counteract the metabolic effects of a high-fat diet (HFD). Results showed that daily administration of D-Limonene (30 and 60 mg/kg body weight) for 84 days decreased the weight gain of HFD mice. After 84 days we observed a statistically significant difference in weight gain in the group of mice receiving the higher dose of FLS compared to HFD mice (35.24 ± 4.56 g vs. 40.79 ± 3.28 g, <i>p</i> < 0.05). Moreover, FLS at both doses tested was capable of lowering triglyceridemia and also fasting glycemia at the higher dose. Some insights on the relevant fatty acid changes in hepatic tissues were obtained, highlighting the increased polyunsaturated fatty acid (PUFA) levels even at the lowest dose. FLS was also able to positively modulate the gut microbiota and prevent HFD-associated liver steatosis in a dose-dependent manner. These results demonstrate that FLS at these doses can be considered non-toxic and could be an effective tool to counteract diet-induced obesity and ameliorate metabolic profile in mice.
format	article
author	Maria Chiara Valerii Silvia Turroni Carla Ferreri Michela Zaro Anna Sansone Alessandro Dalpiaz Giada Botti Luca Ferraro Renato Spigarelli Irene Bellocchio Federica D’Amico Enzo Spisni
author_facet	Maria Chiara Valerii Silvia Turroni Carla Ferreri Michela Zaro Anna Sansone Alessandro Dalpiaz Giada Botti Luca Ferraro Renato Spigarelli Irene Bellocchio Federica D’Amico Enzo Spisni
author_sort	Maria Chiara Valerii
title	Effect of a Fiber D-Limonene-Enriched Food Supplement on Intestinal Microbiota and Metabolic Parameters of Mice on a High-Fat Diet
title_short	Effect of a Fiber D-Limonene-Enriched Food Supplement on Intestinal Microbiota and Metabolic Parameters of Mice on a High-Fat Diet
title_full	Effect of a Fiber D-Limonene-Enriched Food Supplement on Intestinal Microbiota and Metabolic Parameters of Mice on a High-Fat Diet
title_fullStr	Effect of a Fiber D-Limonene-Enriched Food Supplement on Intestinal Microbiota and Metabolic Parameters of Mice on a High-Fat Diet
title_full_unstemmed	Effect of a Fiber D-Limonene-Enriched Food Supplement on Intestinal Microbiota and Metabolic Parameters of Mice on a High-Fat Diet
title_sort	effect of a fiber d-limonene-enriched food supplement on intestinal microbiota and metabolic parameters of mice on a high-fat diet
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ad844570d81347adbcf8478c2e2f06f2
work_keys_str_mv	AT mariachiaravalerii effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT silviaturroni effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT carlaferreri effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT michelazaro effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT annasansone effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT alessandrodalpiaz effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT giadabotti effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT lucaferraro effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT renatospigarelli effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT irenebellocchio effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT federicadamico effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet AT enzospisni effectofafiberdlimoneneenrichedfoodsupplementonintestinalmicrobiotaandmetabolicparametersofmiceonahighfatdiet
_version_	1718410857087500288

Effect of a Fiber D-Limonene-Enriched Food Supplement on Intestinal Microbiota and Metabolic Parameters of Mice on a High-Fat Diet

Ejemplares similares