Periocular injection of candesartan-PLGA microparticles inhibits laser-induced experimental choroidal neovascularization

Yoshitaka Okuda,1 Masanori Fukumoto,1 Taeko Horie,1 Hidehiro Oku,1 Shinji Takai,2 Toyofumi Nakanishi,3 Kaori Matsuzaki,4 Hiroyuki Tsujimoto,4 Tsunehiko Ikeda1 1Department of Ophthalmology, Osaka Medical College, Osaka, Japan; 2Department of Innovative Medicine, Graduate School of Medicine, Osaka Me...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Okuda Y, Fukumoto M, Horie T, Oku H, Takai S, Nakanishi T, Matsuzaki K, Tsujimoto H, Ikeda T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	age-related macular degeneration choroidal neovascularization renin-angiotensin system candesartan macrophage monocyte chemotactic protein 1 poly(lactic-co-glycolic acid) Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/ad84eb589c4a4a2799b83e144de1db28
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ad84eb589c4a4a2799b83e144de1db28
record_format	dspace
spelling	oai:doaj.org-article:ad84eb589c4a4a2799b83e144de1db282021-12-02T02:47:49ZPeriocular injection of candesartan-PLGA microparticles inhibits laser-induced experimental choroidal neovascularization1177-5483https://doaj.org/article/ad84eb589c4a4a2799b83e144de1db282018-12-01T00:00:00Zhttps://www.dovepress.com/periocular-injection-of-candesartan-plga-microparticles-inhibits-laser-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Yoshitaka Okuda,1 Masanori Fukumoto,1 Taeko Horie,1 Hidehiro Oku,1 Shinji Takai,2 Toyofumi Nakanishi,3 Kaori Matsuzaki,4 Hiroyuki Tsujimoto,4 Tsunehiko Ikeda1 1Department of Ophthalmology, Osaka Medical College, Osaka, Japan; 2Department of Innovative Medicine, Graduate School of Medicine, Osaka Medical College, Osaka, Japan; 3Department of Clinical and Laboratory Medicine, Osaka Medical College, Osaka, Japan; 4Research and Development Division, Hosokawa Micron Corporation, Osaka, Japan Purpose: Microparticle technology enables local administration of medication. The purpose of this study was to examine the inhibitory effect of locally administered candesartan (CAN)-encapsulated microparticles on experimental choroidal neovascularization (CNV). Methods: Laser photocoagulation was used to induce CNV in Brown Norway rats. The rats were pretreated with subconjunctival injections of CAN (5.0 mg/eye) or phosphate buffer saline for 3 days before photocoagulation. The volume of CNV was evaluated 7 days after laser injury using the lectin staining technique. The infiltration of macrophages within the CNV lesion was determined using immunofluorescent staining with an anti-CD68 antibody. mRNA levels of MCP-1, IL1-β and VEGF in the retinal pigment epithelium/choroid complex were determined using quantitative PCR (q-PCR). Results: CNV volume was significantly suppressed by the treatment with CAN compared with that in vehicle-treated eyes (P<0.05, two-tailed Student’s t-test). Subconjunctival injections of CAN decreased the numbers of CD68+ cells in the CNV lesion. The increased mRNA levels of MCP-1, IL1-β, and VEGF induced by photocoagulation was significantly suppressed following the local administration of CAN (P<0.05, two-tailed Student’s t-test). Conclusion: Local administration of CAN inhibited experimentally induced CNV possibly through anti-inflammatory effects. Keywords: age-related macular degeneration, choroidal neovascularization, renin-angiotensin system, candesartan, macrophage, monocyte chemotactic protein 1, poly(lactic-co-glycolic acid)Okuda YFukumoto MHorie TOku HTakai SNakanishi TMatsuzaki KTsujimoto HIkeda TDove Medical Pressarticleage-related macular degenerationchoroidal neovascularizationrenin-angiotensin systemcandesartanmacrophagemonocyte chemotactic protein 1poly(lactic-co-glycolic acid)OphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 13, Pp 87-93 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	age-related macular degeneration choroidal neovascularization renin-angiotensin system candesartan macrophage monocyte chemotactic protein 1 poly(lactic-co-glycolic acid) Ophthalmology RE1-994
spellingShingle	age-related macular degeneration choroidal neovascularization renin-angiotensin system candesartan macrophage monocyte chemotactic protein 1 poly(lactic-co-glycolic acid) Ophthalmology RE1-994 Okuda Y Fukumoto M Horie T Oku H Takai S Nakanishi T Matsuzaki K Tsujimoto H Ikeda T Periocular injection of candesartan-PLGA microparticles inhibits laser-induced experimental choroidal neovascularization
description	Yoshitaka Okuda,1 Masanori Fukumoto,1 Taeko Horie,1 Hidehiro Oku,1 Shinji Takai,2 Toyofumi Nakanishi,3 Kaori Matsuzaki,4 Hiroyuki Tsujimoto,4 Tsunehiko Ikeda1 1Department of Ophthalmology, Osaka Medical College, Osaka, Japan; 2Department of Innovative Medicine, Graduate School of Medicine, Osaka Medical College, Osaka, Japan; 3Department of Clinical and Laboratory Medicine, Osaka Medical College, Osaka, Japan; 4Research and Development Division, Hosokawa Micron Corporation, Osaka, Japan Purpose: Microparticle technology enables local administration of medication. The purpose of this study was to examine the inhibitory effect of locally administered candesartan (CAN)-encapsulated microparticles on experimental choroidal neovascularization (CNV). Methods: Laser photocoagulation was used to induce CNV in Brown Norway rats. The rats were pretreated with subconjunctival injections of CAN (5.0 mg/eye) or phosphate buffer saline for 3 days before photocoagulation. The volume of CNV was evaluated 7 days after laser injury using the lectin staining technique. The infiltration of macrophages within the CNV lesion was determined using immunofluorescent staining with an anti-CD68 antibody. mRNA levels of MCP-1, IL1-β and VEGF in the retinal pigment epithelium/choroid complex were determined using quantitative PCR (q-PCR). Results: CNV volume was significantly suppressed by the treatment with CAN compared with that in vehicle-treated eyes (P<0.05, two-tailed Student’s t-test). Subconjunctival injections of CAN decreased the numbers of CD68+ cells in the CNV lesion. The increased mRNA levels of MCP-1, IL1-β, and VEGF induced by photocoagulation was significantly suppressed following the local administration of CAN (P<0.05, two-tailed Student’s t-test). Conclusion: Local administration of CAN inhibited experimentally induced CNV possibly through anti-inflammatory effects. Keywords: age-related macular degeneration, choroidal neovascularization, renin-angiotensin system, candesartan, macrophage, monocyte chemotactic protein 1, poly(lactic-co-glycolic acid)
format	article
author	Okuda Y Fukumoto M Horie T Oku H Takai S Nakanishi T Matsuzaki K Tsujimoto H Ikeda T
author_facet	Okuda Y Fukumoto M Horie T Oku H Takai S Nakanishi T Matsuzaki K Tsujimoto H Ikeda T
author_sort	Okuda Y
title	Periocular injection of candesartan-PLGA microparticles inhibits laser-induced experimental choroidal neovascularization
title_short	Periocular injection of candesartan-PLGA microparticles inhibits laser-induced experimental choroidal neovascularization
title_full	Periocular injection of candesartan-PLGA microparticles inhibits laser-induced experimental choroidal neovascularization
title_fullStr	Periocular injection of candesartan-PLGA microparticles inhibits laser-induced experimental choroidal neovascularization
title_full_unstemmed	Periocular injection of candesartan-PLGA microparticles inhibits laser-induced experimental choroidal neovascularization
title_sort	periocular injection of candesartan-plga microparticles inhibits laser-induced experimental choroidal neovascularization
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/ad84eb589c4a4a2799b83e144de1db28
work_keys_str_mv	AT okuday periocularinjectionofcandesartanplgamicroparticlesinhibitslaserinducedexperimentalchoroidalneovascularization AT fukumotom periocularinjectionofcandesartanplgamicroparticlesinhibitslaserinducedexperimentalchoroidalneovascularization AT horiet periocularinjectionofcandesartanplgamicroparticlesinhibitslaserinducedexperimentalchoroidalneovascularization AT okuh periocularinjectionofcandesartanplgamicroparticlesinhibitslaserinducedexperimentalchoroidalneovascularization AT takais periocularinjectionofcandesartanplgamicroparticlesinhibitslaserinducedexperimentalchoroidalneovascularization AT nakanishit periocularinjectionofcandesartanplgamicroparticlesinhibitslaserinducedexperimentalchoroidalneovascularization AT matsuzakik periocularinjectionofcandesartanplgamicroparticlesinhibitslaserinducedexperimentalchoroidalneovascularization AT tsujimotoh periocularinjectionofcandesartanplgamicroparticlesinhibitslaserinducedexperimentalchoroidalneovascularization AT ikedat periocularinjectionofcandesartanplgamicroparticlesinhibitslaserinducedexperimentalchoroidalneovascularization
_version_	1718402183661092864

Periocular injection of candesartan-PLGA microparticles inhibits laser-induced experimental choroidal neovascularization

Ejemplares similares