rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions

Current methods to unveil protein–protein and protein–RNA interactions often involve long, costly procedures. Here the authors present a rapid yeast 2- and 3-hybrid screening pipeline to detect interactions within protein libraries or between protein libraries and RNA fragment pools.

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Autores principales:	Jae-Seong Yang, Mireia Garriga-Canut, Nele Link, Carlo Carolis, Katrina Broadbent, Violeta Beltran-Sastre, Luis Serrano, Sebastian P. Maurer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ad8f61c4cf2f48e2a8c5190bb415f3f9
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spelling	oai:doaj.org-article:ad8f61c4cf2f48e2a8c5190bb415f3f92021-12-02T15:34:04Zrec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions10.1038/s41467-018-06128-x2041-1723https://doaj.org/article/ad8f61c4cf2f48e2a8c5190bb415f3f92018-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06128-xhttps://doaj.org/toc/2041-1723Current methods to unveil protein–protein and protein–RNA interactions often involve long, costly procedures. Here the authors present a rapid yeast 2- and 3-hybrid screening pipeline to detect interactions within protein libraries or between protein libraries and RNA fragment pools.Jae-Seong YangMireia Garriga-CanutNele LinkCarlo CarolisKatrina BroadbentVioleta Beltran-SastreLuis SerranoSebastian P. MaurerNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jae-Seong Yang Mireia Garriga-Canut Nele Link Carlo Carolis Katrina Broadbent Violeta Beltran-Sastre Luis Serrano Sebastian P. Maurer rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions
description	Current methods to unveil protein–protein and protein–RNA interactions often involve long, costly procedures. Here the authors present a rapid yeast 2- and 3-hybrid screening pipeline to detect interactions within protein libraries or between protein libraries and RNA fragment pools.
format	article
author	Jae-Seong Yang Mireia Garriga-Canut Nele Link Carlo Carolis Katrina Broadbent Violeta Beltran-Sastre Luis Serrano Sebastian P. Maurer
author_facet	Jae-Seong Yang Mireia Garriga-Canut Nele Link Carlo Carolis Katrina Broadbent Violeta Beltran-Sastre Luis Serrano Sebastian P. Maurer
author_sort	Jae-Seong Yang
title	rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions
title_short	rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions
title_full	rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions
title_fullStr	rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions
title_full_unstemmed	rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions
title_sort	rec-ynh enables simultaneous many-by-many detection of direct protein–protein and protein–rna interactions
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ad8f61c4cf2f48e2a8c5190bb415f3f9
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rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions

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