Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)

The present research focuses mainly on molecular mining and morphological evaluation of guava genome within a full-sib population and, thereby, mapping of quantitative trait loci related to fruit quality traits, viz., seed strength (hardness/softness) and average fruit weight. Linkage maps were enri...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	B Padmakar, C Kanupriya, P Madhavi Latha, C Vasugi, M R Dinesh, D Sailaja, C Aswath
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2016
Materias:	composite interval mapping guava linkage map pseudo-testcross quantitative trait loci (qtl) Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/ad94024d38094b0b81feb2e97c5ebc79
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ad94024d38094b0b81feb2e97c5ebc79
record_format	dspace
spelling	oai:doaj.org-article:ad94024d38094b0b81feb2e97c5ebc792021-12-02T12:52:11ZEnrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)0973-354X2582-4899https://doaj.org/article/ad94024d38094b0b81feb2e97c5ebc792016-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/96https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899The present research focuses mainly on molecular mining and morphological evaluation of guava genome within a full-sib population and, thereby, mapping of quantitative trait loci related to fruit quality traits, viz., seed strength (hardness/softness) and average fruit weight. Linkage maps were enriched for both parental lines, 'Kamsari' and 'Purple Local' using a set of 60 RAPD markers following the pseudo-testcross strategy on a panel of 94 progeny. A total of 480 scorable markers were identified, of which 131 were specific to 'kamsari' and 28 to 'Purple Local', segregating as test cross markers, and, 321 showing intercross pattern common to both. 'Kamsari' spanned a total length of 1959.1cM with average marker interval distance of 3.93cM, while 'Purple Local' spanned a length of 1537.9cM with average marker interval distance of 3.29cM, by forming 11 linkage groups. Estimated genome length observed was 93.02% and 92.77% in 'Kamsari' and 'Purple Local', respectively. Composite Interval Mapping (CIM) was computed at significance of 0.05 and LOD threshold greater than 3.0, which led to detection of one major QTL for the trait of average fruit weight, and, four QTLs for the trait of seed strength (hardness/softness). Of these, two were major and two minor QTLs. Our study provides molecular mapping information on marker-assisted selection for improvement of guava in a breeding program.B PadmakarC KanupriyaP Madhavi LathaC VasugiM R DineshD SailajaC AswathSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlecomposite interval mappingguavalinkage mappseudo-testcrossquantitative trait loci (qtl)Plant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 11, Iss 1, Pp 13-20 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	composite interval mapping guava linkage map pseudo-testcross quantitative trait loci (qtl) Plant culture SB1-1110
spellingShingle	composite interval mapping guava linkage map pseudo-testcross quantitative trait loci (qtl) Plant culture SB1-1110 B Padmakar C Kanupriya P Madhavi Latha C Vasugi M R Dinesh D Sailaja C Aswath Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)
description	The present research focuses mainly on molecular mining and morphological evaluation of guava genome within a full-sib population and, thereby, mapping of quantitative trait loci related to fruit quality traits, viz., seed strength (hardness/softness) and average fruit weight. Linkage maps were enriched for both parental lines, 'Kamsari' and 'Purple Local' using a set of 60 RAPD markers following the pseudo-testcross strategy on a panel of 94 progeny. A total of 480 scorable markers were identified, of which 131 were specific to 'kamsari' and 28 to 'Purple Local', segregating as test cross markers, and, 321 showing intercross pattern common to both. 'Kamsari' spanned a total length of 1959.1cM with average marker interval distance of 3.93cM, while 'Purple Local' spanned a length of 1537.9cM with average marker interval distance of 3.29cM, by forming 11 linkage groups. Estimated genome length observed was 93.02% and 92.77% in 'Kamsari' and 'Purple Local', respectively. Composite Interval Mapping (CIM) was computed at significance of 0.05 and LOD threshold greater than 3.0, which led to detection of one major QTL for the trait of average fruit weight, and, four QTLs for the trait of seed strength (hardness/softness). Of these, two were major and two minor QTLs. Our study provides molecular mapping information on marker-assisted selection for improvement of guava in a breeding program.
format	article
author	B Padmakar C Kanupriya P Madhavi Latha C Vasugi M R Dinesh D Sailaja C Aswath
author_facet	B Padmakar C Kanupriya P Madhavi Latha C Vasugi M R Dinesh D Sailaja C Aswath
author_sort	B Padmakar
title	Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)
title_short	Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)
title_full	Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)
title_fullStr	Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)
title_full_unstemmed	Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)
title_sort	enrichment of genetic linkage maps and mapping qtls specific to seed strength-hardness/softness-in guava (psidium guajava l.)
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2016
url	https://doaj.org/article/ad94024d38094b0b81feb2e97c5ebc79
work_keys_str_mv	AT bpadmakar enrichmentofgeneticlinkagemapsandmappingqtlsspecifictoseedstrengthhardnesssoftnessinguavapsidiumguajaval AT ckanupriya enrichmentofgeneticlinkagemapsandmappingqtlsspecifictoseedstrengthhardnesssoftnessinguavapsidiumguajaval AT pmadhavilatha enrichmentofgeneticlinkagemapsandmappingqtlsspecifictoseedstrengthhardnesssoftnessinguavapsidiumguajaval AT cvasugi enrichmentofgeneticlinkagemapsandmappingqtlsspecifictoseedstrengthhardnesssoftnessinguavapsidiumguajaval AT mrdinesh enrichmentofgeneticlinkagemapsandmappingqtlsspecifictoseedstrengthhardnesssoftnessinguavapsidiumguajaval AT dsailaja enrichmentofgeneticlinkagemapsandmappingqtlsspecifictoseedstrengthhardnesssoftnessinguavapsidiumguajaval AT caswath enrichmentofgeneticlinkagemapsandmappingqtlsspecifictoseedstrengthhardnesssoftnessinguavapsidiumguajaval
_version_	1718393644533153792

Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)

Ejemplares similares