A novel intronic circular RNA, circGNG7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser82

Abstract Background There is increasing evidence that circular RNAs (circRNAs) play a significant role in pathological processes including tumorigenesis. In contrast to exonic circRNAs, which are the most frequently reported circRNAs in cancer so far, the studies of intronic circRNAs have been great...

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Autores principales:	Houyu Ju, Zhenrong Hu, Dongliang Wei, Jinyun Huang, Xinyi Zhang, Mengyu Rui, Zhi Li, Xiaomeng Zhang, Jingzhou Hu, Wei Guo, Guoxin Ren
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	circGNG7 head and neck squamous cell carcinoma heat shock protein 27 (HSP27) intronic circular RNAs mitogen‐activated protein kinase (MAPK) signaling phosphorylation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/ad95132c5f9b4223851980ce677315d3
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spelling	oai:doaj.org-article:ad95132c5f9b4223851980ce677315d32021-11-27T06:05:32ZA novel intronic circular RNA, circGNG7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser822523-354810.1002/cac2.12213https://doaj.org/article/ad95132c5f9b4223851980ce677315d32021-11-01T00:00:00Zhttps://doi.org/10.1002/cac2.12213https://doaj.org/toc/2523-3548Abstract Background There is increasing evidence that circular RNAs (circRNAs) play a significant role in pathological processes including tumorigenesis. In contrast to exonic circRNAs, which are the most frequently reported circRNAs in cancer so far, the studies of intronic circRNAs have been greatly lagged behind. Here, we aimed to investigate the regulatory role of intronic circRNAs in head and neck squamous cell carcinoma (HNSCC). Methods We conducted whole‐transcriptome sequencing with four pairs of primary tumor tissues and adjacent normal tissues from HNSCC patients. Then, we characterized circGNG7 expression in HNSCC tissues and cell lines and explored its association with the prognosis of HNSCC patients. We also identified interactions between circGNG7 and functional proteins, which alter downstream signaling that regulate HNSCC progression. Results In this study, we identified a new intronic circRNA, circGNG7, and validated its functional roles in HNSCC progression. CircGNG7 was predominately localized to the cytoplasm, and its expression was downregulated in both HNSCC tissues andCAL27, CAL33, SCC4, SCC9, HN6, and HN30 cells. Low expression of circGNG7 was significantly correlated with poor prognosis in HNSCC patients. Consistent with this finding, overexpression of circGNG7 strongly inhibited tumor cell proliferation, colony formation, in vitro migration, and in vivo tumor growth. Mechanistically, the expression of circGNG7 in HNSCC cells was regulated by the transcription factor SMAD family member 4 (SMAD4). Importantly, we discovered that circGNG7 could bind to serine residues 78 and 82 of the functional heat shock protein 27 (HSP27), occupying its phosphorylation sites and hindering its phosphorylation, which reduced HSP27‐JNK/P38 mitogen‐activated protein kinase (MAPK) oncogenic signaling. Downregulation of circGNG7 expression in HNSCC increased HSP27‐JNK/P38 MAPK signaling and promoted tumor progression. Conclusions Our results revealed that a new intronic circRNA, circGNG7, functions as a strong tumor suppressor and that circGNG7/HSP27‐JNK/P38 MAPK signaling is a novel mechanism by which HNSCC progression can be controlled.Houyu JuZhenrong HuDongliang WeiJinyun HuangXinyi ZhangMengyu RuiZhi LiXiaomeng ZhangJingzhou HuWei GuoGuoxin RenWileyarticlecircGNG7head and neck squamous cell carcinomaheat shock protein 27 (HSP27)intronic circular RNAsmitogen‐activated protein kinase (MAPK) signalingphosphorylationNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancer Communications, Vol 41, Iss 11, Pp 1152-1172 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circGNG7 head and neck squamous cell carcinoma heat shock protein 27 (HSP27) intronic circular RNAs mitogen‐activated protein kinase (MAPK) signaling phosphorylation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	circGNG7 head and neck squamous cell carcinoma heat shock protein 27 (HSP27) intronic circular RNAs mitogen‐activated protein kinase (MAPK) signaling phosphorylation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Houyu Ju Zhenrong Hu Dongliang Wei Jinyun Huang Xinyi Zhang Mengyu Rui Zhi Li Xiaomeng Zhang Jingzhou Hu Wei Guo Guoxin Ren A novel intronic circular RNA, circGNG7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser82
description	Abstract Background There is increasing evidence that circular RNAs (circRNAs) play a significant role in pathological processes including tumorigenesis. In contrast to exonic circRNAs, which are the most frequently reported circRNAs in cancer so far, the studies of intronic circRNAs have been greatly lagged behind. Here, we aimed to investigate the regulatory role of intronic circRNAs in head and neck squamous cell carcinoma (HNSCC). Methods We conducted whole‐transcriptome sequencing with four pairs of primary tumor tissues and adjacent normal tissues from HNSCC patients. Then, we characterized circGNG7 expression in HNSCC tissues and cell lines and explored its association with the prognosis of HNSCC patients. We also identified interactions between circGNG7 and functional proteins, which alter downstream signaling that regulate HNSCC progression. Results In this study, we identified a new intronic circRNA, circGNG7, and validated its functional roles in HNSCC progression. CircGNG7 was predominately localized to the cytoplasm, and its expression was downregulated in both HNSCC tissues andCAL27, CAL33, SCC4, SCC9, HN6, and HN30 cells. Low expression of circGNG7 was significantly correlated with poor prognosis in HNSCC patients. Consistent with this finding, overexpression of circGNG7 strongly inhibited tumor cell proliferation, colony formation, in vitro migration, and in vivo tumor growth. Mechanistically, the expression of circGNG7 in HNSCC cells was regulated by the transcription factor SMAD family member 4 (SMAD4). Importantly, we discovered that circGNG7 could bind to serine residues 78 and 82 of the functional heat shock protein 27 (HSP27), occupying its phosphorylation sites and hindering its phosphorylation, which reduced HSP27‐JNK/P38 mitogen‐activated protein kinase (MAPK) oncogenic signaling. Downregulation of circGNG7 expression in HNSCC increased HSP27‐JNK/P38 MAPK signaling and promoted tumor progression. Conclusions Our results revealed that a new intronic circRNA, circGNG7, functions as a strong tumor suppressor and that circGNG7/HSP27‐JNK/P38 MAPK signaling is a novel mechanism by which HNSCC progression can be controlled.
format	article
author	Houyu Ju Zhenrong Hu Dongliang Wei Jinyun Huang Xinyi Zhang Mengyu Rui Zhi Li Xiaomeng Zhang Jingzhou Hu Wei Guo Guoxin Ren
author_facet	Houyu Ju Zhenrong Hu Dongliang Wei Jinyun Huang Xinyi Zhang Mengyu Rui Zhi Li Xiaomeng Zhang Jingzhou Hu Wei Guo Guoxin Ren
author_sort	Houyu Ju
title	A novel intronic circular RNA, circGNG7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser82
title_short	A novel intronic circular RNA, circGNG7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser82
title_full	A novel intronic circular RNA, circGNG7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser82
title_fullStr	A novel intronic circular RNA, circGNG7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser82
title_full_unstemmed	A novel intronic circular RNA, circGNG7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser82
title_sort	novel intronic circular rna, circgng7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at ser78 and ser82
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/ad95132c5f9b4223851980ce677315d3
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A novel intronic circular RNA, circGNG7, inhibits head and neck squamous cell carcinoma progression by blocking the phosphorylation of heat shock protein 27 at Ser78 and Ser82

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