A Novel Circular RNA circPTCD3 Promotes Breast Cancer Progression Through Sponging miR-198

Zhaohui Zhang,1 Hao Hu,2 Qian Li,1 Fumei Yi,1 Yan’e Liu1 1Department of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Beijing, 100191, People’s Republic of China; 2Beijing 100biotech Co., Ltd., Beijing, 100006, People’s Republic of ChinaCorrespondence: Zhaohui ZhangDep...

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Autores principales:	Zhang Z, Hu H, Li Q, Yi F, Liu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	circptcd3 breast cancer mir-198 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/ad960a0b1ef840eab6e093e087324848
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spelling	oai:doaj.org-article:ad960a0b1ef840eab6e093e0873248482021-11-09T18:40:35ZA Novel Circular RNA circPTCD3 Promotes Breast Cancer Progression Through Sponging miR-1981179-1322https://doaj.org/article/ad960a0b1ef840eab6e093e0873248482021-11-01T00:00:00Zhttps://www.dovepress.com/a-novel-circular-rna-circptcd3-promotes-breast-cancer-progression-thro-peer-reviewed-fulltext-article-CMARhttps://doaj.org/toc/1179-1322Zhaohui Zhang,1 Hao Hu,2 Qian Li,1 Fumei Yi,1 Yan’e Liu1 1Department of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Beijing, 100191, People’s Republic of China; 2Beijing 100biotech Co., Ltd., Beijing, 100006, People’s Republic of ChinaCorrespondence: Zhaohui ZhangDepartment of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Huayuan Bei Road, Haidian District, Beijing, 100191, People’s Republic of ChinaEmail zhaobj429@sina.comIntroduction: Circular RNAs (circRNAs), a new type of non-coding RNA, have been demonstrated to play critical roles in the progression of various of malignant cancers. However, the function of circRNAs in breast cancer is not clearly understood.Methods: qPCR was used to evaluate the gene expression. Function studies including MTT, transwell, wound healing and colony formation assay were performed to evaluate the function of circPTCD3 in breast cancer. Luciferase and RNA pull-down assays were used to verify the interaction between circPTCD3 and miR-198. The xenograft model was established to evaluate the function of circPTCD3 in vivo.Results: In the present study, we identified a novel circRNA termed as circPTCD3 which was indicated to be significantly up-regulated in breast cancer tissues and cell lines. The results revealed that ectopic expression of circPTCD3 promoted the cell proliferation, migration and colony formation ability of breast cancer cells. Constantly, silencing of circPTCD3 inhibited those of breast cancer cells. Furthermore, we identified that circPTCD3 was able to target miR-198 in breast cancer cell. miR-198 has the function of inhibiting proliferation and migration of breast cancer cells which can be reversed by circPTCD3.Conclusion: Taken together, our findings for the first time identified a novel circRNA (circPTCD3) and revealed its oncogenic role in breast cancer. Mechanically, we reported that circPTCD3 served as a competing endogenous RNA (ceRNA) to sponge miR-198. These findings provide insights into breast cancer progression and also potential new targets for diagnosis or treatment of breast cancer.Keywords: circPTCD3, breast cancer, miR-198Zhang ZHu HLi QYi FLiu YDove Medical Pressarticlecircptcd3breast cancermir-198Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancer Management and Research, Vol Volume 13, Pp 8435-8443 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circptcd3 breast cancer mir-198 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	circptcd3 breast cancer mir-198 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Zhang Z Hu H Li Q Yi F Liu Y A Novel Circular RNA circPTCD3 Promotes Breast Cancer Progression Through Sponging miR-198
description	Zhaohui Zhang,1 Hao Hu,2 Qian Li,1 Fumei Yi,1 Yan’e Liu1 1Department of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Beijing, 100191, People’s Republic of China; 2Beijing 100biotech Co., Ltd., Beijing, 100006, People’s Republic of ChinaCorrespondence: Zhaohui ZhangDepartment of Tumor Chemotherapy and Radiation Sickness, Peking University Third Hospital, Huayuan Bei Road, Haidian District, Beijing, 100191, People’s Republic of ChinaEmail zhaobj429@sina.comIntroduction: Circular RNAs (circRNAs), a new type of non-coding RNA, have been demonstrated to play critical roles in the progression of various of malignant cancers. However, the function of circRNAs in breast cancer is not clearly understood.Methods: qPCR was used to evaluate the gene expression. Function studies including MTT, transwell, wound healing and colony formation assay were performed to evaluate the function of circPTCD3 in breast cancer. Luciferase and RNA pull-down assays were used to verify the interaction between circPTCD3 and miR-198. The xenograft model was established to evaluate the function of circPTCD3 in vivo.Results: In the present study, we identified a novel circRNA termed as circPTCD3 which was indicated to be significantly up-regulated in breast cancer tissues and cell lines. The results revealed that ectopic expression of circPTCD3 promoted the cell proliferation, migration and colony formation ability of breast cancer cells. Constantly, silencing of circPTCD3 inhibited those of breast cancer cells. Furthermore, we identified that circPTCD3 was able to target miR-198 in breast cancer cell. miR-198 has the function of inhibiting proliferation and migration of breast cancer cells which can be reversed by circPTCD3.Conclusion: Taken together, our findings for the first time identified a novel circRNA (circPTCD3) and revealed its oncogenic role in breast cancer. Mechanically, we reported that circPTCD3 served as a competing endogenous RNA (ceRNA) to sponge miR-198. These findings provide insights into breast cancer progression and also potential new targets for diagnosis or treatment of breast cancer.Keywords: circPTCD3, breast cancer, miR-198
format	article
author	Zhang Z Hu H Li Q Yi F Liu Y
author_facet	Zhang Z Hu H Li Q Yi F Liu Y
author_sort	Zhang Z
title	A Novel Circular RNA circPTCD3 Promotes Breast Cancer Progression Through Sponging miR-198
title_short	A Novel Circular RNA circPTCD3 Promotes Breast Cancer Progression Through Sponging miR-198
title_full	A Novel Circular RNA circPTCD3 Promotes Breast Cancer Progression Through Sponging miR-198
title_fullStr	A Novel Circular RNA circPTCD3 Promotes Breast Cancer Progression Through Sponging miR-198
title_full_unstemmed	A Novel Circular RNA circPTCD3 Promotes Breast Cancer Progression Through Sponging miR-198
title_sort	novel circular rna circptcd3 promotes breast cancer progression through sponging mir-198
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/ad960a0b1ef840eab6e093e087324848
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A Novel Circular RNA circPTCD3 Promotes Breast Cancer Progression Through Sponging miR-198

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