Rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish.

During meiosis I, ring-shaped cohesin complexes play important roles in aiding the proper segregation of homologous chromosomes. RAD21L is a meiosis-specific vertebrate cohesin that is required for spermatogenesis in mice but is dispensable for oogenesis in young animals. The role of this cohesin in...

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Autores principales:	Yana P Blokhina, Michelle A Frees, An Nguyen, Masuda Sharifi, Daniel B Chu, Kristi Bispo, Ivan Olaya, Bruce W Draper, Sean M Burgess
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/ad9a19ea73c14e888eeb0165e5f38c46
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spelling	oai:doaj.org-article:ad9a19ea73c14e888eeb0165e5f38c462021-12-02T20:02:41ZRad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish.1553-73901553-740410.1371/journal.pgen.1009127https://doaj.org/article/ad9a19ea73c14e888eeb0165e5f38c462021-06-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009127https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404During meiosis I, ring-shaped cohesin complexes play important roles in aiding the proper segregation of homologous chromosomes. RAD21L is a meiosis-specific vertebrate cohesin that is required for spermatogenesis in mice but is dispensable for oogenesis in young animals. The role of this cohesin in other vertebrate models has not been explored. Here, we tested if the zebrafish homolog Rad21l1 is required for meiotic chromosome dynamics during spermatogenesis and oogenesis. We found that Rad21l1 localizes to unsynapsed chromosome axes. It is also found between the axes of the mature tripartite synaptonemal complex (SC) in both sexes. We knocked out rad21l1 and found that nearly all rad21l1-/- mutants develop as fertile males, suggesting that the mutation causes a defect in juvenile oogenesis, since insufficient oocyte production triggers female to male sex reversal in zebrafish. Sex reversal was partially suppressed by mutation of the checkpoint gene tp53, suggesting that the rad21l1 mutation activates Tp53-mediated apoptosis or arrest in females. This response, however, is not linked to a defect in repairing Spo11-induced double-strand breaks since deletion of spo11 does not suppress the sex reversal phenotype. Compared to tp53 single mutant controls, rad21l1-/- tp53-/- double mutant females produce poor quality eggs that often die or develop into malformed embryos. Overall, these results indicate that the absence of rad21l1-/- females is due to a checkpoint-mediated response and highlight a role for a meiotic-specific cohesin subunit in oogenesis but not spermatogenesis.Yana P BlokhinaMichelle A FreesAn NguyenMasuda SharifiDaniel B ChuKristi BispoIvan OlayaBruce W DraperSean M BurgessPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 6, p e1009127 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Yana P Blokhina Michelle A Frees An Nguyen Masuda Sharifi Daniel B Chu Kristi Bispo Ivan Olaya Bruce W Draper Sean M Burgess Rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish.
description	During meiosis I, ring-shaped cohesin complexes play important roles in aiding the proper segregation of homologous chromosomes. RAD21L is a meiosis-specific vertebrate cohesin that is required for spermatogenesis in mice but is dispensable for oogenesis in young animals. The role of this cohesin in other vertebrate models has not been explored. Here, we tested if the zebrafish homolog Rad21l1 is required for meiotic chromosome dynamics during spermatogenesis and oogenesis. We found that Rad21l1 localizes to unsynapsed chromosome axes. It is also found between the axes of the mature tripartite synaptonemal complex (SC) in both sexes. We knocked out rad21l1 and found that nearly all rad21l1-/- mutants develop as fertile males, suggesting that the mutation causes a defect in juvenile oogenesis, since insufficient oocyte production triggers female to male sex reversal in zebrafish. Sex reversal was partially suppressed by mutation of the checkpoint gene tp53, suggesting that the rad21l1 mutation activates Tp53-mediated apoptosis or arrest in females. This response, however, is not linked to a defect in repairing Spo11-induced double-strand breaks since deletion of spo11 does not suppress the sex reversal phenotype. Compared to tp53 single mutant controls, rad21l1-/- tp53-/- double mutant females produce poor quality eggs that often die or develop into malformed embryos. Overall, these results indicate that the absence of rad21l1-/- females is due to a checkpoint-mediated response and highlight a role for a meiotic-specific cohesin subunit in oogenesis but not spermatogenesis.
format	article
author	Yana P Blokhina Michelle A Frees An Nguyen Masuda Sharifi Daniel B Chu Kristi Bispo Ivan Olaya Bruce W Draper Sean M Burgess
author_facet	Yana P Blokhina Michelle A Frees An Nguyen Masuda Sharifi Daniel B Chu Kristi Bispo Ivan Olaya Bruce W Draper Sean M Burgess
author_sort	Yana P Blokhina
title	Rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish.
title_short	Rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish.
title_full	Rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish.
title_fullStr	Rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish.
title_full_unstemmed	Rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish.
title_sort	rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ad9a19ea73c14e888eeb0165e5f38c46
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Rad21l1 cohesin subunit is dispensable for spermatogenesis but not oogenesis in zebrafish.

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