High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser

Abstract Here we demonstrate high-pulse-energy multiphoton microscopy (MPM) for intravital imaging of neurons and oligodendrocytes in the murine brain. Pulses with an order of magnitude higher energy (~ 10 nJ) were employed from a ytterbium doped fiber laser source at a 1-MHz repetition rate, as com...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Michael J. Redlich, Brad Prall, Edesly Canto-Said, Yevgeniy Busarov, Lilit Shirinyan-Tuka, Arafat Meah, Hyungsik Lim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ada1bbfad5e24d6683789106e4ac8cae
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ada1bbfad5e24d6683789106e4ac8cae
record_format	dspace
spelling	oai:doaj.org-article:ada1bbfad5e24d6683789106e4ac8cae2021-12-02T14:26:16ZHigh-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser10.1038/s41598-021-86924-62045-2322https://doaj.org/article/ada1bbfad5e24d6683789106e4ac8cae2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86924-6https://doaj.org/toc/2045-2322Abstract Here we demonstrate high-pulse-energy multiphoton microscopy (MPM) for intravital imaging of neurons and oligodendrocytes in the murine brain. Pulses with an order of magnitude higher energy (~ 10 nJ) were employed from a ytterbium doped fiber laser source at a 1-MHz repetition rate, as compared to the standard 80-MHz Ti:Sapphire laser. Intravital imaging was performed on mice expressing common fluorescent proteins, including green (GFP) and yellow fluorescent proteins (YFP), and TagRFPt. One fifth of the average power could be used for superior depths of MPM imaging, as compared to the Ti:Sapphire laser: A depth of ~ 860 µm was obtained by imaging the Thy1-YFP brain in vivo with 6.5 mW, and cortical myelin as deep as 400 µm ex vivo by intrinsic third-harmonic generation using 50 mW. The substantially higher pulse energy enables novel regimes of photophysics to be exploited for microscopic imaging. The limitation from higher order phototoxicity is also discussed.Michael J. RedlichBrad PrallEdesly Canto-SaidYevgeniy BusarovLilit Shirinyan-TukaArafat MeahHyungsik LimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael J. Redlich Brad Prall Edesly Canto-Said Yevgeniy Busarov Lilit Shirinyan-Tuka Arafat Meah Hyungsik Lim High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser
description	Abstract Here we demonstrate high-pulse-energy multiphoton microscopy (MPM) for intravital imaging of neurons and oligodendrocytes in the murine brain. Pulses with an order of magnitude higher energy (~ 10 nJ) were employed from a ytterbium doped fiber laser source at a 1-MHz repetition rate, as compared to the standard 80-MHz Ti:Sapphire laser. Intravital imaging was performed on mice expressing common fluorescent proteins, including green (GFP) and yellow fluorescent proteins (YFP), and TagRFPt. One fifth of the average power could be used for superior depths of MPM imaging, as compared to the Ti:Sapphire laser: A depth of ~ 860 µm was obtained by imaging the Thy1-YFP brain in vivo with 6.5 mW, and cortical myelin as deep as 400 µm ex vivo by intrinsic third-harmonic generation using 50 mW. The substantially higher pulse energy enables novel regimes of photophysics to be exploited for microscopic imaging. The limitation from higher order phototoxicity is also discussed.
format	article
author	Michael J. Redlich Brad Prall Edesly Canto-Said Yevgeniy Busarov Lilit Shirinyan-Tuka Arafat Meah Hyungsik Lim
author_facet	Michael J. Redlich Brad Prall Edesly Canto-Said Yevgeniy Busarov Lilit Shirinyan-Tuka Arafat Meah Hyungsik Lim
author_sort	Michael J. Redlich
title	High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser
title_short	High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser
title_full	High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser
title_fullStr	High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser
title_full_unstemmed	High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser
title_sort	high-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ada1bbfad5e24d6683789106e4ac8cae
work_keys_str_mv	AT michaeljredlich highpulseenergymultiphotonimagingofneuronsandoligodendrocytesindeepmurinebrainwithafiberlaser AT bradprall highpulseenergymultiphotonimagingofneuronsandoligodendrocytesindeepmurinebrainwithafiberlaser AT edeslycantosaid highpulseenergymultiphotonimagingofneuronsandoligodendrocytesindeepmurinebrainwithafiberlaser AT yevgeniybusarov highpulseenergymultiphotonimagingofneuronsandoligodendrocytesindeepmurinebrainwithafiberlaser AT lilitshirinyantuka highpulseenergymultiphotonimagingofneuronsandoligodendrocytesindeepmurinebrainwithafiberlaser AT arafatmeah highpulseenergymultiphotonimagingofneuronsandoligodendrocytesindeepmurinebrainwithafiberlaser AT hyungsiklim highpulseenergymultiphotonimagingofneuronsandoligodendrocytesindeepmurinebrainwithafiberlaser
_version_	1718391389182492672

High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser

Ejemplares similares