Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds

Yunming Tao,1,2 Tiehui Hu,1 Zhongshi Wu,1 Hao Tang,1 Yerong Hu,1 Qi Tan,1 Chunlin Wu11Department of Thoracic and Cardiovascular Surgery, Second Xiangya Hospital of Central South University, Changsha; 2Department of Thoracic and Cardiovascular Surgery, Ji&#39;an Central People&rsquo;s...

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Autores principales:	Tao Y, Hu T, Wu Z, Tang H, Hu Y, Tan Q, Wu C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ada2e839a187431c9ceb3ba8d0d846b3
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spelling	oai:doaj.org-article:ada2e839a187431c9ceb3ba8d0d846b32021-12-02T02:10:27ZHeparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds1176-91141178-2013https://doaj.org/article/ada2e839a187431c9ceb3ba8d0d846b32012-11-01T00:00:00Zhttp://www.dovepress.com/heparin-nanomodification-improves-biocompatibility-and-biomechanical-s-a11605https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yunming Tao,1,2 Tiehui Hu,1 Zhongshi Wu,1 Hao Tang,1 Yerong Hu,1 Qi Tan,1 Chunlin Wu11Department of Thoracic and Cardiovascular Surgery, Second Xiangya Hospital of Central South University, Changsha; 2Department of Thoracic and Cardiovascular Surgery, Ji&#39;an Central People&rsquo;s Hospital, Ji&#39;an, Jiangxi Province, People&#39;s Republic of ChinaAbstract: Biocompatibility and biomechanical stability are two of the main obstacles limiting the effectiveness of vascular scaffolds. To improve the biomechanical stability and biocompatibility of these scaffolds, we created a heparin-nanomodified acellular bovine jugular vein scaffold by alternating linkage of heparin and dihydroxy-iron via self-assembly. Features of the scaffold were evaluated in vitro and in vivo. Heparin was firmly linked to and formed nanoscale coatings around the fibers of the scaffold, and the amount of heparin linked was about 808 &plusmn; 86 &micro;g/cm2 (101 &plusmn; 11 USP/cm2) per assembly cycle. The scaffolds showed significantly strengthened biomechanical stability with sustained release of heparin for several weeks in vitro. Importantly, the modified scaffolds showed significantly reduced platelet adhesion, stimulated proliferation of endothelial cells in vitro, and reduced calcification in a subcutaneous implantation rat model in vivo. Heparin nanomodification improves the biocompatibility and biomechanical stability of vascular scaffolds.Keywords: scaffolds, nanomodification, heparin, sustained release, biomechanical stability, biocompatibilityTao YHu TWu ZTang HHu YTan QWu CDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5847-5858 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Tao Y Hu T Wu Z Tang H Hu Y Tan Q Wu C Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds
description	Yunming Tao,1,2 Tiehui Hu,1 Zhongshi Wu,1 Hao Tang,1 Yerong Hu,1 Qi Tan,1 Chunlin Wu11Department of Thoracic and Cardiovascular Surgery, Second Xiangya Hospital of Central South University, Changsha; 2Department of Thoracic and Cardiovascular Surgery, Ji&#39;an Central People&rsquo;s Hospital, Ji&#39;an, Jiangxi Province, People&#39;s Republic of ChinaAbstract: Biocompatibility and biomechanical stability are two of the main obstacles limiting the effectiveness of vascular scaffolds. To improve the biomechanical stability and biocompatibility of these scaffolds, we created a heparin-nanomodified acellular bovine jugular vein scaffold by alternating linkage of heparin and dihydroxy-iron via self-assembly. Features of the scaffold were evaluated in vitro and in vivo. Heparin was firmly linked to and formed nanoscale coatings around the fibers of the scaffold, and the amount of heparin linked was about 808 &plusmn; 86 &micro;g/cm2 (101 &plusmn; 11 USP/cm2) per assembly cycle. The scaffolds showed significantly strengthened biomechanical stability with sustained release of heparin for several weeks in vitro. Importantly, the modified scaffolds showed significantly reduced platelet adhesion, stimulated proliferation of endothelial cells in vitro, and reduced calcification in a subcutaneous implantation rat model in vivo. Heparin nanomodification improves the biocompatibility and biomechanical stability of vascular scaffolds.Keywords: scaffolds, nanomodification, heparin, sustained release, biomechanical stability, biocompatibility
format	article
author	Tao Y Hu T Wu Z Tang H Hu Y Tan Q Wu C
author_facet	Tao Y Hu T Wu Z Tang H Hu Y Tan Q Wu C
author_sort	Tao Y
title	Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds
title_short	Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds
title_full	Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds
title_fullStr	Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds
title_full_unstemmed	Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds
title_sort	heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/ada2e839a187431c9ceb3ba8d0d846b3
work_keys_str_mv	AT taoy heparinnanomodificationimprovesbiocompatibilityandbiomechanicalstabilityofdecellularizedvascularscaffolds AT hut heparinnanomodificationimprovesbiocompatibilityandbiomechanicalstabilityofdecellularizedvascularscaffolds AT wuz heparinnanomodificationimprovesbiocompatibilityandbiomechanicalstabilityofdecellularizedvascularscaffolds AT tangh heparinnanomodificationimprovesbiocompatibilityandbiomechanicalstabilityofdecellularizedvascularscaffolds AT huy heparinnanomodificationimprovesbiocompatibilityandbiomechanicalstabilityofdecellularizedvascularscaffolds AT tanq heparinnanomodificationimprovesbiocompatibilityandbiomechanicalstabilityofdecellularizedvascularscaffolds AT wuc heparinnanomodificationimprovesbiocompatibilityandbiomechanicalstabilityofdecellularizedvascularscaffolds
_version_	1718402669396099072

Heparin nanomodification improves biocompatibility and biomechanical stability of decellularized vascular scaffolds

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