Temporal changes of Sall4 lineage contribution in developing embryos and the contribution of Sall4-lineages to postnatal germ cells in mice

Abstract Mutations in the SALL4 gene cause human syndromes with defects in multiple organs. Sall4 expression declines rapidly in post-gastrulation mouse embryos, and our understanding of the requirement of Sall4 in animal development is still limited. To assess the contributions of Sall4 expressing...

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Autores principales:	Naoyuki Tahara, Hiroko Kawakami, Teng Zhang, David Zarkower, Yasuhiko Kawakami
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Sales Line Sal Expression Spermatogonial Stem Cells (SSCs) Salivary mRNA Tamoxifen Injection Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ada8956bffef4fcd98ae5fa82504f6bc
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spelling	oai:doaj.org-article:ada8956bffef4fcd98ae5fa82504f6bc2021-12-02T15:09:02ZTemporal changes of Sall4 lineage contribution in developing embryos and the contribution of Sall4-lineages to postnatal germ cells in mice10.1038/s41598-018-34745-52045-2322https://doaj.org/article/ada8956bffef4fcd98ae5fa82504f6bc2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34745-5https://doaj.org/toc/2045-2322Abstract Mutations in the SALL4 gene cause human syndromes with defects in multiple organs. Sall4 expression declines rapidly in post-gastrulation mouse embryos, and our understanding of the requirement of Sall4 in animal development is still limited. To assess the contributions of Sall4 expressing cells to developing mouse embryos, we monitored temporal changes of the contribution of Sall4 lineages using a Sall4 GFP-CreER T2 knock-in mouse line and recombination-dependent reporter lines. By administering tamoxifen at various time points we observed that the contributions of Sall4 lineages to the axial level were rapidly restricted from the entire body to the posterior part of the body. The contribution to forelimbs, hindlimbs, craniofacial structures and external genitalia also declined after gastrulation with different temporal dynamics. We also detected Sall4 lineage contributions to the extra-embryonic tissues, such as the yolk sac and umbilical cord, in a temporal manner. These Sall4 lineage contributions provide insights into potential roles of Sall4 during mammalian embryonic development. In postnatal males, long-term lineage tracing detected Sall4 lineage contributions to the spermatogonial stem cell pool during spermatogenesis. The Sall4 GFP-CreER T2 line can serve as a tool to monitor spatial-temporal contributions of Sall4 lineages as well as to perform gene manipulations in Sall4-expressing lineages.Naoyuki TaharaHiroko KawakamiTeng ZhangDavid ZarkowerYasuhiko KawakamiNature PortfolioarticleSales LineSal ExpressionSpermatogonial Stem Cells (SSCs)Salivary mRNATamoxifen InjectionMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sales Line Sal Expression Spermatogonial Stem Cells (SSCs) Salivary mRNA Tamoxifen Injection Medicine R Science Q
spellingShingle	Sales Line Sal Expression Spermatogonial Stem Cells (SSCs) Salivary mRNA Tamoxifen Injection Medicine R Science Q Naoyuki Tahara Hiroko Kawakami Teng Zhang David Zarkower Yasuhiko Kawakami Temporal changes of Sall4 lineage contribution in developing embryos and the contribution of Sall4-lineages to postnatal germ cells in mice
description	Abstract Mutations in the SALL4 gene cause human syndromes with defects in multiple organs. Sall4 expression declines rapidly in post-gastrulation mouse embryos, and our understanding of the requirement of Sall4 in animal development is still limited. To assess the contributions of Sall4 expressing cells to developing mouse embryos, we monitored temporal changes of the contribution of Sall4 lineages using a Sall4 GFP-CreER T2 knock-in mouse line and recombination-dependent reporter lines. By administering tamoxifen at various time points we observed that the contributions of Sall4 lineages to the axial level were rapidly restricted from the entire body to the posterior part of the body. The contribution to forelimbs, hindlimbs, craniofacial structures and external genitalia also declined after gastrulation with different temporal dynamics. We also detected Sall4 lineage contributions to the extra-embryonic tissues, such as the yolk sac and umbilical cord, in a temporal manner. These Sall4 lineage contributions provide insights into potential roles of Sall4 during mammalian embryonic development. In postnatal males, long-term lineage tracing detected Sall4 lineage contributions to the spermatogonial stem cell pool during spermatogenesis. The Sall4 GFP-CreER T2 line can serve as a tool to monitor spatial-temporal contributions of Sall4 lineages as well as to perform gene manipulations in Sall4-expressing lineages.
format	article
author	Naoyuki Tahara Hiroko Kawakami Teng Zhang David Zarkower Yasuhiko Kawakami
author_facet	Naoyuki Tahara Hiroko Kawakami Teng Zhang David Zarkower Yasuhiko Kawakami
author_sort	Naoyuki Tahara
title	Temporal changes of Sall4 lineage contribution in developing embryos and the contribution of Sall4-lineages to postnatal germ cells in mice
title_short	Temporal changes of Sall4 lineage contribution in developing embryos and the contribution of Sall4-lineages to postnatal germ cells in mice
title_full	Temporal changes of Sall4 lineage contribution in developing embryos and the contribution of Sall4-lineages to postnatal germ cells in mice
title_fullStr	Temporal changes of Sall4 lineage contribution in developing embryos and the contribution of Sall4-lineages to postnatal germ cells in mice
title_full_unstemmed	Temporal changes of Sall4 lineage contribution in developing embryos and the contribution of Sall4-lineages to postnatal germ cells in mice
title_sort	temporal changes of sall4 lineage contribution in developing embryos and the contribution of sall4-lineages to postnatal germ cells in mice
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ada8956bffef4fcd98ae5fa82504f6bc
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_version_	1718387958583656448

Temporal changes of Sall4 lineage contribution in developing embryos and the contribution of Sall4-lineages to postnatal germ cells in mice

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