Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from <i>Arthrospira platensis</i>

To investigate the structure of <i>Arthrospira platensis</i> polysaccharide (PAP) (intracellular polysaccharide) and the antioxidant activity of the first component of PAP (PAP-1) on pseudorabies virus (PRV) -infected RAW264.7 cells. The PAP was separated and purified by the Cellulose DE...

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Autores principales:	Mi-Xia Cao, Xiao-Dong Xie, Xin-Rui Wang, Wen-Yue Hu, Yi Zhao, Qi Chen, Lu Ji, Ying-Yi Wei, Mei-Ling Yu, Ting-Jun Hu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Arthrospira platensis</i> polysaccharide pseudorabies virus antioxidant structure circRNA-miRNA-mRNA Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ada9c1614b674ad5829b28c98c2778f4
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spelling	oai:doaj.org-article:ada9c1614b674ad5829b28c98c2778f42021-11-25T16:26:13ZSeparation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from <i>Arthrospira platensis</i>10.3390/antiox101116892076-3921https://doaj.org/article/ada9c1614b674ad5829b28c98c2778f42021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1689https://doaj.org/toc/2076-3921To investigate the structure of <i>Arthrospira platensis</i> polysaccharide (PAP) (intracellular polysaccharide) and the antioxidant activity of the first component of PAP (PAP-1) on pseudorabies virus (PRV) -infected RAW264.7 cells. The PAP was separated and purified by the Cellulose DE-52 chromatography column and Sephacryl S-200 high-resolution gel column to obtain PAP-1. The antioxidant activity and regulation of PAP-1 on PRV-infected RAW264.7 cells of circRNA-miRNA-mRNA network were investigated by chemical kit, Q-PCR, and ce-RNA seq. The results indicated that the molecular weight (Mw) of PAP-1, which was mainly composed of glucose and eight other monosaccharides, was 1.48 × 10<sup>6</sup> Da. The main glycosidic bond structure of PAP-1 was →4)-α-D-Glcp-(1→. PAP-1 may be increased the antioxidant capacity by regulating the circRNA-miRNA-mRNA network in PRV-infected RAW264.7 cells. This study provided a scientific foundation for further exploring the antioxidant activity of PAP-1 based on its structure.Mi-Xia CaoXiao-Dong XieXin-Rui WangWen-Yue HuYi ZhaoQi ChenLu JiYing-Yi WeiMei-Ling YuTing-Jun HuMDPI AGarticle<i>Arthrospira platensis</i> polysaccharidepseudorabies virusantioxidantstructurecircRNA-miRNA-mRNATherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1689, p 1689 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Arthrospira platensis</i> polysaccharide pseudorabies virus antioxidant structure circRNA-miRNA-mRNA Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Arthrospira platensis</i> polysaccharide pseudorabies virus antioxidant structure circRNA-miRNA-mRNA Therapeutics. Pharmacology RM1-950 Mi-Xia Cao Xiao-Dong Xie Xin-Rui Wang Wen-Yue Hu Yi Zhao Qi Chen Lu Ji Ying-Yi Wei Mei-Ling Yu Ting-Jun Hu Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from <i>Arthrospira platensis</i>
description	To investigate the structure of <i>Arthrospira platensis</i> polysaccharide (PAP) (intracellular polysaccharide) and the antioxidant activity of the first component of PAP (PAP-1) on pseudorabies virus (PRV) -infected RAW264.7 cells. The PAP was separated and purified by the Cellulose DE-52 chromatography column and Sephacryl S-200 high-resolution gel column to obtain PAP-1. The antioxidant activity and regulation of PAP-1 on PRV-infected RAW264.7 cells of circRNA-miRNA-mRNA network were investigated by chemical kit, Q-PCR, and ce-RNA seq. The results indicated that the molecular weight (Mw) of PAP-1, which was mainly composed of glucose and eight other monosaccharides, was 1.48 × 10<sup>6</sup> Da. The main glycosidic bond structure of PAP-1 was →4)-α-D-Glcp-(1→. PAP-1 may be increased the antioxidant capacity by regulating the circRNA-miRNA-mRNA network in PRV-infected RAW264.7 cells. This study provided a scientific foundation for further exploring the antioxidant activity of PAP-1 based on its structure.
format	article
author	Mi-Xia Cao Xiao-Dong Xie Xin-Rui Wang Wen-Yue Hu Yi Zhao Qi Chen Lu Ji Ying-Yi Wei Mei-Ling Yu Ting-Jun Hu
author_facet	Mi-Xia Cao Xiao-Dong Xie Xin-Rui Wang Wen-Yue Hu Yi Zhao Qi Chen Lu Ji Ying-Yi Wei Mei-Ling Yu Ting-Jun Hu
author_sort	Mi-Xia Cao
title	Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from <i>Arthrospira platensis</i>
title_short	Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from <i>Arthrospira platensis</i>
title_full	Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from <i>Arthrospira platensis</i>
title_fullStr	Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from <i>Arthrospira platensis</i>
title_full_unstemmed	Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from <i>Arthrospira platensis</i>
title_sort	separation, purification, structure analysis, in vitro antioxidant activity and circrna-mirna-mrna regulatory network on prv-infected raw264.7 cells of a polysaccharide derived from <i>arthrospira platensis</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ada9c1614b674ad5829b28c98c2778f4
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Separation, Purification, Structure Analysis, In Vitro Antioxidant Activity and circRNA-miRNA-mRNA Regulatory Network on PRV-Infected RAW264.7 Cells of a Polysaccharide Derived from <i>Arthrospira platensis</i>

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