Renalase deficiency in heart failure model of rats--a potential mechanism underlying circulating norepinephrine accumulation.

<h4>Background</h4>Sympathetic overactivity and catecholamine accumulation are important characteristic findings in heart failure, which contribute to its pathophysiology. Here, we identify a potential mechanism underlying norepinephrine accumulation in a rat model of heart failure.<h...

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Autores principales:	Rong Gu, Wen Lu, Jun Xie, Jian Bai, Biao Xu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/adad94df698740c9a91e90f90e03d3e0
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spelling	oai:doaj.org-article:adad94df698740c9a91e90f90e03d3e02021-11-18T06:59:33ZRenalase deficiency in heart failure model of rats--a potential mechanism underlying circulating norepinephrine accumulation.1932-620310.1371/journal.pone.0014633https://doaj.org/article/adad94df698740c9a91e90f90e03d3e02011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21297953/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Sympathetic overactivity and catecholamine accumulation are important characteristic findings in heart failure, which contribute to its pathophysiology. Here, we identify a potential mechanism underlying norepinephrine accumulation in a rat model of heart failure.<h4>Methodology/principal findings</h4>Initially, we constructed a rat model of unilateral renal artery stenosis (n = 16) and found that the expression of renalase, a previously identified secreted amine oxidase, was markedly reduced in the ischemic compared to the non-ischemic kidney (protein: 0.295±0.085 versus 0.765±0.171, p<0.05). Subsequently, we utilized an isolated perfused rat kidney model to demonstrate that the clearance rate of norepinephrine decreased with reduction of perfusion flow. On the basis of these findings, we hypothesized the reduced renal blood supply which occurs in heart failure would result in impaired synthesis of renalase by the kidney and consequently reduced degradation of circulating norepinephrine. To verify this, we used a rat model of infarction-induced heart failure (n = 12 per group). In these rats, the flow velocity of renal artery, when measured at four weeks, is obviously lower in the operation group. Renal expression of renalase was reduced (protein: 0.476±0.043 for control, 0.248±0.029 for operation versus 0.636±0.151 for sham-operation) and this was associated with an increase in circulating norepinephrine (0.168±0.016 ng/mL for control, 0.203±0.019 ng/mL for operation versus 0.138±0.008 ng/mL for sham-operation).<h4>Conclusions/significance</h4>Renalase expression is influenced by renal blood flow and impaired synthesis of renalase by the kidney may represent a potential mechanism underlying circulating norepinephrine accumulation in heart failure.Rong GuWen LuJun XieJian BaiBiao XuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e14633 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rong Gu Wen Lu Jun Xie Jian Bai Biao Xu Renalase deficiency in heart failure model of rats--a potential mechanism underlying circulating norepinephrine accumulation.
description	<h4>Background</h4>Sympathetic overactivity and catecholamine accumulation are important characteristic findings in heart failure, which contribute to its pathophysiology. Here, we identify a potential mechanism underlying norepinephrine accumulation in a rat model of heart failure.<h4>Methodology/principal findings</h4>Initially, we constructed a rat model of unilateral renal artery stenosis (n = 16) and found that the expression of renalase, a previously identified secreted amine oxidase, was markedly reduced in the ischemic compared to the non-ischemic kidney (protein: 0.295±0.085 versus 0.765±0.171, p<0.05). Subsequently, we utilized an isolated perfused rat kidney model to demonstrate that the clearance rate of norepinephrine decreased with reduction of perfusion flow. On the basis of these findings, we hypothesized the reduced renal blood supply which occurs in heart failure would result in impaired synthesis of renalase by the kidney and consequently reduced degradation of circulating norepinephrine. To verify this, we used a rat model of infarction-induced heart failure (n = 12 per group). In these rats, the flow velocity of renal artery, when measured at four weeks, is obviously lower in the operation group. Renal expression of renalase was reduced (protein: 0.476±0.043 for control, 0.248±0.029 for operation versus 0.636±0.151 for sham-operation) and this was associated with an increase in circulating norepinephrine (0.168±0.016 ng/mL for control, 0.203±0.019 ng/mL for operation versus 0.138±0.008 ng/mL for sham-operation).<h4>Conclusions/significance</h4>Renalase expression is influenced by renal blood flow and impaired synthesis of renalase by the kidney may represent a potential mechanism underlying circulating norepinephrine accumulation in heart failure.
format	article
author	Rong Gu Wen Lu Jun Xie Jian Bai Biao Xu
author_facet	Rong Gu Wen Lu Jun Xie Jian Bai Biao Xu
author_sort	Rong Gu
title	Renalase deficiency in heart failure model of rats--a potential mechanism underlying circulating norepinephrine accumulation.
title_short	Renalase deficiency in heart failure model of rats--a potential mechanism underlying circulating norepinephrine accumulation.
title_full	Renalase deficiency in heart failure model of rats--a potential mechanism underlying circulating norepinephrine accumulation.
title_fullStr	Renalase deficiency in heart failure model of rats--a potential mechanism underlying circulating norepinephrine accumulation.
title_full_unstemmed	Renalase deficiency in heart failure model of rats--a potential mechanism underlying circulating norepinephrine accumulation.
title_sort	renalase deficiency in heart failure model of rats--a potential mechanism underlying circulating norepinephrine accumulation.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/adad94df698740c9a91e90f90e03d3e0
work_keys_str_mv	AT ronggu renalasedeficiencyinheartfailuremodelofratsapotentialmechanismunderlyingcirculatingnorepinephrineaccumulation AT wenlu renalasedeficiencyinheartfailuremodelofratsapotentialmechanismunderlyingcirculatingnorepinephrineaccumulation AT junxie renalasedeficiencyinheartfailuremodelofratsapotentialmechanismunderlyingcirculatingnorepinephrineaccumulation AT jianbai renalasedeficiencyinheartfailuremodelofratsapotentialmechanismunderlyingcirculatingnorepinephrineaccumulation AT biaoxu renalasedeficiencyinheartfailuremodelofratsapotentialmechanismunderlyingcirculatingnorepinephrineaccumulation
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Renalase deficiency in heart failure model of rats--a potential mechanism underlying circulating norepinephrine accumulation.

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