MULTIPLEX EVALUATION OF ConA-INDUCED PRODUCTION OF TWELVE CYTOKINES IN THE CULTURES OF MONONUCLEAR CELLS FROM PERIPHERAL BLOOD OF HEALTHY SUBJECTS

Abstract. The goal of this work was a multiplex single-step evaluation of the levels of stimulated cytokine production in the supernates of cultivated mononuclear cells from healthy Caucasian individuals. We compared the levels of cytokines for various groups. In Th1 cells, the production of INF-γ i...

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Autores principales:	V. I. Konenkov, V. V. Avdoshina, I. G. Rakova, M. V. Smolninkova, E. L. Gelfgat
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	cytokines production concanavalin a healthy donors Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/adb1c315b942499cb03fc5e0c654d21d
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spelling	oai:doaj.org-article:adb1c315b942499cb03fc5e0c654d21d2021-11-18T08:03:41ZMULTIPLEX EVALUATION OF ConA-INDUCED PRODUCTION OF TWELVE CYTOKINES IN THE CULTURES OF MONONUCLEAR CELLS FROM PERIPHERAL BLOOD OF HEALTHY SUBJECTS1563-06252313-741X10.15789/1563-0625-2006-4-517-522https://doaj.org/article/adb1c315b942499cb03fc5e0c654d21d2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/479https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The goal of this work was a multiplex single-step evaluation of the levels of stimulated cytokine production in the supernates of cultivated mononuclear cells from healthy Caucasian individuals. We compared the levels of cytokines for various groups. In Th1 cells, the production of INF-γ is typically greater than production of IL-2. Analysis of Th2-derived cytokines demonstrated predominance of IL-10 production over other cytokines. Furthermore, we observed in healthy donors a significantly stronger production of IL-13 as compared to IL-4. These cytokines function as co-stimulators of proliferation for pre-activated B-cells.When performing pairwise comparisons of the growth factor concentrations, we have shown higher levels of G-CSF production than that of GM-CSF, thus reflecting predominance of differentiation stimuli for granulocytopoiesis over monocytopoiesis in bone marrow of healthy humans.Among pro-inflammatory cytokines tested, the significantly higher TNF-α production is prevalent. Low levels of ConA stimulated IL-7 production from the bone marrow stromal cells, which activates complete differentiation of T- and B-lymphocyte progenitors, might be due to low mitogen activity for the non-lymphoid cells.V. I. KonenkovV. V. AvdoshinaI. G. RakovaM. V. SmolninkovaE. L. GelfgatSPb RAACIarticlecytokinesproductionconcanavalin ahealthy donorsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 8, Iss 4, Pp 517-522 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	cytokines production concanavalin a healthy donors Immunologic diseases. Allergy RC581-607
spellingShingle	cytokines production concanavalin a healthy donors Immunologic diseases. Allergy RC581-607 V. I. Konenkov V. V. Avdoshina I. G. Rakova M. V. Smolninkova E. L. Gelfgat MULTIPLEX EVALUATION OF ConA-INDUCED PRODUCTION OF TWELVE CYTOKINES IN THE CULTURES OF MONONUCLEAR CELLS FROM PERIPHERAL BLOOD OF HEALTHY SUBJECTS
description	Abstract. The goal of this work was a multiplex single-step evaluation of the levels of stimulated cytokine production in the supernates of cultivated mononuclear cells from healthy Caucasian individuals. We compared the levels of cytokines for various groups. In Th1 cells, the production of INF-γ is typically greater than production of IL-2. Analysis of Th2-derived cytokines demonstrated predominance of IL-10 production over other cytokines. Furthermore, we observed in healthy donors a significantly stronger production of IL-13 as compared to IL-4. These cytokines function as co-stimulators of proliferation for pre-activated B-cells.When performing pairwise comparisons of the growth factor concentrations, we have shown higher levels of G-CSF production than that of GM-CSF, thus reflecting predominance of differentiation stimuli for granulocytopoiesis over monocytopoiesis in bone marrow of healthy humans.Among pro-inflammatory cytokines tested, the significantly higher TNF-α production is prevalent. Low levels of ConA stimulated IL-7 production from the bone marrow stromal cells, which activates complete differentiation of T- and B-lymphocyte progenitors, might be due to low mitogen activity for the non-lymphoid cells.
format	article
author	V. I. Konenkov V. V. Avdoshina I. G. Rakova M. V. Smolninkova E. L. Gelfgat
author_facet	V. I. Konenkov V. V. Avdoshina I. G. Rakova M. V. Smolninkova E. L. Gelfgat
author_sort	V. I. Konenkov
title	MULTIPLEX EVALUATION OF ConA-INDUCED PRODUCTION OF TWELVE CYTOKINES IN THE CULTURES OF MONONUCLEAR CELLS FROM PERIPHERAL BLOOD OF HEALTHY SUBJECTS
title_short	MULTIPLEX EVALUATION OF ConA-INDUCED PRODUCTION OF TWELVE CYTOKINES IN THE CULTURES OF MONONUCLEAR CELLS FROM PERIPHERAL BLOOD OF HEALTHY SUBJECTS
title_full	MULTIPLEX EVALUATION OF ConA-INDUCED PRODUCTION OF TWELVE CYTOKINES IN THE CULTURES OF MONONUCLEAR CELLS FROM PERIPHERAL BLOOD OF HEALTHY SUBJECTS
title_fullStr	MULTIPLEX EVALUATION OF ConA-INDUCED PRODUCTION OF TWELVE CYTOKINES IN THE CULTURES OF MONONUCLEAR CELLS FROM PERIPHERAL BLOOD OF HEALTHY SUBJECTS
title_full_unstemmed	MULTIPLEX EVALUATION OF ConA-INDUCED PRODUCTION OF TWELVE CYTOKINES IN THE CULTURES OF MONONUCLEAR CELLS FROM PERIPHERAL BLOOD OF HEALTHY SUBJECTS
title_sort	multiplex evaluation of cona-induced production of twelve cytokines in the cultures of mononuclear cells from peripheral blood of healthy subjects
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/adb1c315b942499cb03fc5e0c654d21d
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MULTIPLEX EVALUATION OF ConA-INDUCED PRODUCTION OF TWELVE CYTOKINES IN THE CULTURES OF MONONUCLEAR CELLS FROM PERIPHERAL BLOOD OF HEALTHY SUBJECTS

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