Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation

Abstract Connexins play essential roles in lens homeostasis and development. Here, we identified a new role for Cx50 that mediates cell-cell adhesion function. Cx50 enhanced the adhesive capability of AQP0. Interestingly, the expression of Cx50 alone promoted cell adhesion at a comparable level to A...

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Autores principales:	Zhengping Hu, Wen Shi, Manuel A. Riquelme, Qian Shi, Sondip Biswas, Woo-Kuen Lo, Thomas W. White, Sumin Gu, Jean X. Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/adbf4e47e6d44d61a17a14eb20b331b8
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spelling	oai:doaj.org-article:adbf4e47e6d44d61a17a14eb20b331b82021-12-02T12:32:18ZConnexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation10.1038/s41598-017-05647-92045-2322https://doaj.org/article/adbf4e47e6d44d61a17a14eb20b331b82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05647-9https://doaj.org/toc/2045-2322Abstract Connexins play essential roles in lens homeostasis and development. Here, we identified a new role for Cx50 that mediates cell-cell adhesion function. Cx50 enhanced the adhesive capability of AQP0. Interestingly, the expression of Cx50 alone promoted cell adhesion at a comparable level to AQP0; however, this cell adhesive function was not observed with other lens connexins, Cx43 and Cx46. Moreover, the adhesive property occurred in both homotypic with Cx50 expressed in both pairing cells and heterotypic with Cx50 in only one pairing cell, and this function appears to be unrelated to its role in forming gap junction channels. Cx50 KO lenses exhibited increased intercellular spaces between lens fiber cells. The second extracellular loop domain (E2) is primarily responsible for this adhesive function. Treatment with a fusion protein containing E2 domain inhibited cell adhesion. Furthermore, disruption of cell adhesion by the E2 domains impaired primary lens cell differentiation. Five critical amino acid residues in the E2 domain primarily are involved in cell adhesive function as well as lens epithelial-fiber differentiation. Together, these results suggest that in addition to forming gap junction channels, Cx50 acts as an adhesive molecule that is critical in maintaining lens fiber integrity and epithelial-fiber differentiation.Zhengping HuWen ShiManuel A. RiquelmeQian ShiSondip BiswasWoo-Kuen LoThomas W. WhiteSumin GuJean X. JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhengping Hu Wen Shi Manuel A. Riquelme Qian Shi Sondip Biswas Woo-Kuen Lo Thomas W. White Sumin Gu Jean X. Jiang Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation
description	Abstract Connexins play essential roles in lens homeostasis and development. Here, we identified a new role for Cx50 that mediates cell-cell adhesion function. Cx50 enhanced the adhesive capability of AQP0. Interestingly, the expression of Cx50 alone promoted cell adhesion at a comparable level to AQP0; however, this cell adhesive function was not observed with other lens connexins, Cx43 and Cx46. Moreover, the adhesive property occurred in both homotypic with Cx50 expressed in both pairing cells and heterotypic with Cx50 in only one pairing cell, and this function appears to be unrelated to its role in forming gap junction channels. Cx50 KO lenses exhibited increased intercellular spaces between lens fiber cells. The second extracellular loop domain (E2) is primarily responsible for this adhesive function. Treatment with a fusion protein containing E2 domain inhibited cell adhesion. Furthermore, disruption of cell adhesion by the E2 domains impaired primary lens cell differentiation. Five critical amino acid residues in the E2 domain primarily are involved in cell adhesive function as well as lens epithelial-fiber differentiation. Together, these results suggest that in addition to forming gap junction channels, Cx50 acts as an adhesive molecule that is critical in maintaining lens fiber integrity and epithelial-fiber differentiation.
format	article
author	Zhengping Hu Wen Shi Manuel A. Riquelme Qian Shi Sondip Biswas Woo-Kuen Lo Thomas W. White Sumin Gu Jean X. Jiang
author_facet	Zhengping Hu Wen Shi Manuel A. Riquelme Qian Shi Sondip Biswas Woo-Kuen Lo Thomas W. White Sumin Gu Jean X. Jiang
author_sort	Zhengping Hu
title	Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation
title_short	Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation
title_full	Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation
title_fullStr	Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation
title_full_unstemmed	Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation
title_sort	connexin 50 functions as an adhesive molecule and promotes lens cell differentiation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/adbf4e47e6d44d61a17a14eb20b331b8
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Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation

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