Comparative assessment of mouse models for experimental orthodontic tooth movement

Abstract Animal experiments are essential for the elucidation of biological-cellular mechanisms in the context of orthodontic tooth movement (OTM). So far, however, no studies comparatively assess available mouse models regarding their suitability. OTM of first upper molars was induced in C57BL/6 mi...

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Autores principales:	Christian Kirschneck, Maria Bauer, Joshua Gubernator, Peter Proff, Agnes Schröder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/adc4495c50364b79a4dab09279c55873
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spelling	oai:doaj.org-article:adc4495c50364b79a4dab09279c558732021-12-02T16:26:21ZComparative assessment of mouse models for experimental orthodontic tooth movement10.1038/s41598-020-69030-x2045-2322https://doaj.org/article/adc4495c50364b79a4dab09279c558732020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-69030-xhttps://doaj.org/toc/2045-2322Abstract Animal experiments are essential for the elucidation of biological-cellular mechanisms in the context of orthodontic tooth movement (OTM). So far, however, no studies comparatively assess available mouse models regarding their suitability. OTM of first upper molars was induced in C57BL/6 mice either via an elastic band or a NiTi coil spring for three, seven or 12 days. We assessed appliance survival rate, OTM and periodontal bone loss (µCT), root resorptions, osteoclastogenesis (TRAP+ area) and local expression of OTM-related genes (RT-qPCR). Seven days after the elastic bands were inserted, 87% were still in situ, but only 27% after 12 days. Survival rate for the NiTi coil springs was 100% throughout, but 8.9% of the animals did not survive. Both methods induced significant OTM, which was highest after 12 (NiTi spring) and 7 days (band), with a corresponding increase in local gene expression of OTM-related genes and osteoclastogenesis. Periodontal bone loss and root resorptions were not induced at a relevant extent by neither of the two procedures within the experimental periods. To induce reliable OTM in mice beyond 7 days, a NiTi coil spring is the method of choice. The elastic band method is recommended only for short-term yes/no-questions regarding OTM.Christian KirschneckMaria BauerJoshua GubernatorPeter ProffAgnes SchröderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Kirschneck Maria Bauer Joshua Gubernator Peter Proff Agnes Schröder Comparative assessment of mouse models for experimental orthodontic tooth movement
description	Abstract Animal experiments are essential for the elucidation of biological-cellular mechanisms in the context of orthodontic tooth movement (OTM). So far, however, no studies comparatively assess available mouse models regarding their suitability. OTM of first upper molars was induced in C57BL/6 mice either via an elastic band or a NiTi coil spring for three, seven or 12 days. We assessed appliance survival rate, OTM and periodontal bone loss (µCT), root resorptions, osteoclastogenesis (TRAP+ area) and local expression of OTM-related genes (RT-qPCR). Seven days after the elastic bands were inserted, 87% were still in situ, but only 27% after 12 days. Survival rate for the NiTi coil springs was 100% throughout, but 8.9% of the animals did not survive. Both methods induced significant OTM, which was highest after 12 (NiTi spring) and 7 days (band), with a corresponding increase in local gene expression of OTM-related genes and osteoclastogenesis. Periodontal bone loss and root resorptions were not induced at a relevant extent by neither of the two procedures within the experimental periods. To induce reliable OTM in mice beyond 7 days, a NiTi coil spring is the method of choice. The elastic band method is recommended only for short-term yes/no-questions regarding OTM.
format	article
author	Christian Kirschneck Maria Bauer Joshua Gubernator Peter Proff Agnes Schröder
author_facet	Christian Kirschneck Maria Bauer Joshua Gubernator Peter Proff Agnes Schröder
author_sort	Christian Kirschneck
title	Comparative assessment of mouse models for experimental orthodontic tooth movement
title_short	Comparative assessment of mouse models for experimental orthodontic tooth movement
title_full	Comparative assessment of mouse models for experimental orthodontic tooth movement
title_fullStr	Comparative assessment of mouse models for experimental orthodontic tooth movement
title_full_unstemmed	Comparative assessment of mouse models for experimental orthodontic tooth movement
title_sort	comparative assessment of mouse models for experimental orthodontic tooth movement
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/adc4495c50364b79a4dab09279c55873
work_keys_str_mv	AT christiankirschneck comparativeassessmentofmousemodelsforexperimentalorthodontictoothmovement AT mariabauer comparativeassessmentofmousemodelsforexperimentalorthodontictoothmovement AT joshuagubernator comparativeassessmentofmousemodelsforexperimentalorthodontictoothmovement AT peterproff comparativeassessmentofmousemodelsforexperimentalorthodontictoothmovement AT agnesschroder comparativeassessmentofmousemodelsforexperimentalorthodontictoothmovement
_version_	1718384074462068736

Comparative assessment of mouse models for experimental orthodontic tooth movement

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