Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis

Samir S Shoughy,1 Hind M Alkatan,2,4 Abdulelah A Al-Abdullah,2 Albarah El-Khani,2 Jolanda DF de Groot-Mijnes,3 Khalid F Tabbara1,4,5 1Department of Ophthalmology, The Eye Center and The Eye Foundation for Research in Ophthalmology, 2Department of Pathology & Laboratory Medicine and...

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Autores principales:	Shoughy SS, Alkatan HM, Al-Abdullah AA, El-Khani A, de Groot-Mijnes JDF, Tabbara KF
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Viral anterior uveitis PCR Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/adca4df504aa41519bd27dec0b561693
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spelling	oai:doaj.org-article:adca4df504aa41519bd27dec0b5616932021-12-02T00:33:19ZPolymerase chain reaction in unilateral cases of presumed viral anterior uveitis1177-5483https://doaj.org/article/adca4df504aa41519bd27dec0b5616932015-12-01T00:00:00Zhttps://www.dovepress.com/polymerase-chain-reaction-in-unilateral-cases-of-presumed-viral-anteri-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Samir S Shoughy,1 Hind M Alkatan,2,4 Abdulelah A Al-Abdullah,2 Albarah El-Khani,2 Jolanda DF de Groot-Mijnes,3 Khalid F Tabbara1,4,5 1Department of Ophthalmology, The Eye Center and The Eye Foundation for Research in Ophthalmology, 2Department of Pathology & Laboratory Medicine and Uveitis Division, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia; 3Department of Virology and Ophthalmology, University Medical Center Utrecht, Utrecht, the Netherlands; 4Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia; 5The Wilmer Ophthalmological Institute of The Johns Hopkins University School of Medicine, Baltimore, MD, USA Background and objectives: Anterior uveitis is the most common form of intraocular inflammation. The main aim of this study was to determine the viral etiology in patients with unilateral cases of anterior uveitis.Patients and methods: A total of 12 consecutive patients with the diagnosis of idiopathic unilateral anterior uveitis were included prospectively. Aqueous specimens were obtained from each patient by anterior chamber paracentesis and subjected to the detection of viral DNA/RNA genome by polymerase chain reaction assay for herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein–Barr virus, and rubella virus.Results: There were six male and six female patients. The mean age was 43 years, with an age range of 11–82 years. All 12 cases presented with unilateral anterior uveitis. In four (33%) patients, polymerase chain reaction was positive for viral genome. Two patients were positive for herpes simplex virus type 1, one patient was positive for cytomegalovirus and one for Epstein–Barr virus.Conclusion: Recent molecular diagnostic assays would help in the identification of the causative agent in patients with unilateral anterior uveitis. Keywords: viral anterior uveitis, PCR, herpes simplex virus, cytomegalovirus, diffuse keratic precipitates, anterior chamber paracentesisShoughy SSAlkatan HMAl-Abdullah AAEl-Khani Ade Groot-Mijnes JDFTabbara KFDove Medical PressarticleViralanterior uveitisPCROphthalmologyRE1-994ENClinical Ophthalmology, Vol 2015, Iss Issue 1, Pp 2325-2328 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Viral anterior uveitis PCR Ophthalmology RE1-994
spellingShingle	Viral anterior uveitis PCR Ophthalmology RE1-994 Shoughy SS Alkatan HM Al-Abdullah AA El-Khani A de Groot-Mijnes JDF Tabbara KF Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis
description	Samir S Shoughy,1 Hind M Alkatan,2,4 Abdulelah A Al-Abdullah,2 Albarah El-Khani,2 Jolanda DF de Groot-Mijnes,3 Khalid F Tabbara1,4,5 1Department of Ophthalmology, The Eye Center and The Eye Foundation for Research in Ophthalmology, 2Department of Pathology & Laboratory Medicine and Uveitis Division, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia; 3Department of Virology and Ophthalmology, University Medical Center Utrecht, Utrecht, the Netherlands; 4Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia; 5The Wilmer Ophthalmological Institute of The Johns Hopkins University School of Medicine, Baltimore, MD, USA Background and objectives: Anterior uveitis is the most common form of intraocular inflammation. The main aim of this study was to determine the viral etiology in patients with unilateral cases of anterior uveitis.Patients and methods: A total of 12 consecutive patients with the diagnosis of idiopathic unilateral anterior uveitis were included prospectively. Aqueous specimens were obtained from each patient by anterior chamber paracentesis and subjected to the detection of viral DNA/RNA genome by polymerase chain reaction assay for herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein–Barr virus, and rubella virus.Results: There were six male and six female patients. The mean age was 43 years, with an age range of 11–82 years. All 12 cases presented with unilateral anterior uveitis. In four (33%) patients, polymerase chain reaction was positive for viral genome. Two patients were positive for herpes simplex virus type 1, one patient was positive for cytomegalovirus and one for Epstein–Barr virus.Conclusion: Recent molecular diagnostic assays would help in the identification of the causative agent in patients with unilateral anterior uveitis. Keywords: viral anterior uveitis, PCR, herpes simplex virus, cytomegalovirus, diffuse keratic precipitates, anterior chamber paracentesis
format	article
author	Shoughy SS Alkatan HM Al-Abdullah AA El-Khani A de Groot-Mijnes JDF Tabbara KF
author_facet	Shoughy SS Alkatan HM Al-Abdullah AA El-Khani A de Groot-Mijnes JDF Tabbara KF
author_sort	Shoughy SS
title	Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis
title_short	Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis
title_full	Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis
title_fullStr	Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis
title_full_unstemmed	Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis
title_sort	polymerase chain reaction in unilateral cases of presumed viral anterior uveitis
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/adca4df504aa41519bd27dec0b561693
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_version_	1718403699710099456

Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis

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