Wide screening of phage-displayed libraries identifies immune targets in planta.

Microbe-Associated Molecular Patterns and virulence effectors are recognized by plants as a first step to mount a defence response against potential pathogens. This recognition involves a large family of extracellular membrane receptors and other immune proteins located in different sub-cellular com...

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Autores principales:	Cristina Rioja, Saskia C Van Wees, Keith A Charlton, Corné M J Pieterse, Oscar Lorenzo, Susana García-Sánchez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/adcc005f0f094b53a9d80e2724b2b787
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spelling	oai:doaj.org-article:adcc005f0f094b53a9d80e2724b2b7872021-11-18T07:59:52ZWide screening of phage-displayed libraries identifies immune targets in planta.1932-620310.1371/journal.pone.0054654https://doaj.org/article/adcc005f0f094b53a9d80e2724b2b7872013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23372747/?tool=EBIhttps://doaj.org/toc/1932-6203Microbe-Associated Molecular Patterns and virulence effectors are recognized by plants as a first step to mount a defence response against potential pathogens. This recognition involves a large family of extracellular membrane receptors and other immune proteins located in different sub-cellular compartments. We have used phage-display technology to express and select for Arabidopsis proteins able to bind bacterial pathogens. To rapidly identify microbe-bound phage, we developed a monitoring method based on microarrays. This combined strategy allowed for a genome-wide screening of plant proteins involved in pathogen perception. Two phage libraries for high-throughput selection were constructed from cDNA of plants infected with Pseudomonas aeruginosa PA14, or from combined samples of the virulent isolate DC3000 of Pseudomonas syringae pv. tomato and its avirulent variant avrRpt2. These three pathosystems represent different degrees in the specificity of plant-microbe interactions. Libraries cover up to 2 × 10(7) different plant transcripts that can be displayed as functional proteins on the surface of T7 bacteriophage. A number of these were selected in a bio-panning assay for binding to Pseudomonas cells. Among the selected clones we isolated the ethylene response factor ATERF-1, which was able to bind the three bacterial strains in competition assays. ATERF-1 was rapidly exported from the nucleus upon infiltration of either alive or heat-killed Pseudomonas. Moreover, aterf-1 mutants exhibited enhanced susceptibility to infection. These findings suggest that ATERF-1 contains a microbe-recognition domain with a role in plant defence. To identify other putative pathogen-binding proteins on a genome-wide scale, the copy number of selected-vs.-total clones was compared by hybridizing phage cDNAs with Arabidopsis microarrays. Microarray analysis revealed a set of 472 candidates with significant fold change. Within this set defence-related genes, including well-known targets of bacterial effectors, are over-represented. Other genes non-previously related to defence can be associated through this study with general or strain-specific recognition of Pseudomonas.Cristina RiojaSaskia C Van WeesKeith A CharltonCorné M J PieterseOscar LorenzoSusana García-SánchezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54654 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristina Rioja Saskia C Van Wees Keith A Charlton Corné M J Pieterse Oscar Lorenzo Susana García-Sánchez Wide screening of phage-displayed libraries identifies immune targets in planta.
description	Microbe-Associated Molecular Patterns and virulence effectors are recognized by plants as a first step to mount a defence response against potential pathogens. This recognition involves a large family of extracellular membrane receptors and other immune proteins located in different sub-cellular compartments. We have used phage-display technology to express and select for Arabidopsis proteins able to bind bacterial pathogens. To rapidly identify microbe-bound phage, we developed a monitoring method based on microarrays. This combined strategy allowed for a genome-wide screening of plant proteins involved in pathogen perception. Two phage libraries for high-throughput selection were constructed from cDNA of plants infected with Pseudomonas aeruginosa PA14, or from combined samples of the virulent isolate DC3000 of Pseudomonas syringae pv. tomato and its avirulent variant avrRpt2. These three pathosystems represent different degrees in the specificity of plant-microbe interactions. Libraries cover up to 2 × 10(7) different plant transcripts that can be displayed as functional proteins on the surface of T7 bacteriophage. A number of these were selected in a bio-panning assay for binding to Pseudomonas cells. Among the selected clones we isolated the ethylene response factor ATERF-1, which was able to bind the three bacterial strains in competition assays. ATERF-1 was rapidly exported from the nucleus upon infiltration of either alive or heat-killed Pseudomonas. Moreover, aterf-1 mutants exhibited enhanced susceptibility to infection. These findings suggest that ATERF-1 contains a microbe-recognition domain with a role in plant defence. To identify other putative pathogen-binding proteins on a genome-wide scale, the copy number of selected-vs.-total clones was compared by hybridizing phage cDNAs with Arabidopsis microarrays. Microarray analysis revealed a set of 472 candidates with significant fold change. Within this set defence-related genes, including well-known targets of bacterial effectors, are over-represented. Other genes non-previously related to defence can be associated through this study with general or strain-specific recognition of Pseudomonas.
format	article
author	Cristina Rioja Saskia C Van Wees Keith A Charlton Corné M J Pieterse Oscar Lorenzo Susana García-Sánchez
author_facet	Cristina Rioja Saskia C Van Wees Keith A Charlton Corné M J Pieterse Oscar Lorenzo Susana García-Sánchez
author_sort	Cristina Rioja
title	Wide screening of phage-displayed libraries identifies immune targets in planta.
title_short	Wide screening of phage-displayed libraries identifies immune targets in planta.
title_full	Wide screening of phage-displayed libraries identifies immune targets in planta.
title_fullStr	Wide screening of phage-displayed libraries identifies immune targets in planta.
title_full_unstemmed	Wide screening of phage-displayed libraries identifies immune targets in planta.
title_sort	wide screening of phage-displayed libraries identifies immune targets in planta.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/adcc005f0f094b53a9d80e2724b2b787
work_keys_str_mv	AT cristinarioja widescreeningofphagedisplayedlibrariesidentifiesimmunetargetsinplanta AT saskiacvanwees widescreeningofphagedisplayedlibrariesidentifiesimmunetargetsinplanta AT keithacharlton widescreeningofphagedisplayedlibrariesidentifiesimmunetargetsinplanta AT cornemjpieterse widescreeningofphagedisplayedlibrariesidentifiesimmunetargetsinplanta AT oscarlorenzo widescreeningofphagedisplayedlibrariesidentifiesimmunetargetsinplanta AT susanagarciasanchez widescreeningofphagedisplayedlibrariesidentifiesimmunetargetsinplanta
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Wide screening of phage-displayed libraries identifies immune targets in planta.

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