A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites
As said by former United Nations Secretary-General Kofi Annan, “Snakebite is the most important tropical disease you’ve never heard of.” Listed as a priority neglected tropical disease by the World Health Organization, snakebite envenoming (SBE) kills in excess of 125,000 people per year. However, d...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:add56f49384e49e99ccde196caf6f4772021-11-09T05:37:30ZA Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites1664-322410.3389/fimmu.2021.775678https://doaj.org/article/add56f49384e49e99ccde196caf6f4772021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.775678/fullhttps://doaj.org/toc/1664-3224As said by former United Nations Secretary-General Kofi Annan, “Snakebite is the most important tropical disease you’ve never heard of.” Listed as a priority neglected tropical disease by the World Health Organization, snakebite envenoming (SBE) kills in excess of 125,000 people per year. However, due to the complexity and overlap of snake venom compositions, few reliable venom diagnostic methods for genus-/species-specific identification, which is crucial for successful SBE therapy, are available. Here, we develop a strategy to select and prepare genus-specific snake venom antibodies, which allows rapid and efficient clinical diagnosis of snakebite. Multi-omics approaches are used to choose candidate antigens from snake venoms and identify genus-specific antigenic epitope peptide fragments (GSAEPs) with ideal immunogenicity, specificity, and spatial accessibility. Double-antibody sandwich ELISA kit was established by matching a polyclonal antibody against a natural antigen and a monoclonal antibody that was prepared by natural protein as antigen and can specifically target the GSAEPs. The kit shows the ability to accurately identify venoms from similar genera of Trimeresurus and Protobothrops with a detection limit of 6.25 ng/ml on the snake venoms and a little cross-reaction, thus proving high feasibility and applicability.Chengbo LongChengbo LongFeilong WuFeilong WuQiumin LuQiumin LuBing XieChuanbin ShenJiayao LiYanling DengPing LiangYongzhi YuRen LaiRen LaiFrontiers Media S.A.articlesnakebite envenomingvenomantigenimmunogenicitydiagnosisImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021) |
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| collection |
DOAJ |
| language |
EN |
| topic |
snakebite envenoming venom antigen immunogenicity diagnosis Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
snakebite envenoming venom antigen immunogenicity diagnosis Immunologic diseases. Allergy RC581-607 Chengbo Long Chengbo Long Feilong Wu Feilong Wu Qiumin Lu Qiumin Lu Bing Xie Chuanbin Shen Jiayao Li Yanling Deng Ping Liang Yongzhi Yu Ren Lai Ren Lai A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites |
| description |
As said by former United Nations Secretary-General Kofi Annan, “Snakebite is the most important tropical disease you’ve never heard of.” Listed as a priority neglected tropical disease by the World Health Organization, snakebite envenoming (SBE) kills in excess of 125,000 people per year. However, due to the complexity and overlap of snake venom compositions, few reliable venom diagnostic methods for genus-/species-specific identification, which is crucial for successful SBE therapy, are available. Here, we develop a strategy to select and prepare genus-specific snake venom antibodies, which allows rapid and efficient clinical diagnosis of snakebite. Multi-omics approaches are used to choose candidate antigens from snake venoms and identify genus-specific antigenic epitope peptide fragments (GSAEPs) with ideal immunogenicity, specificity, and spatial accessibility. Double-antibody sandwich ELISA kit was established by matching a polyclonal antibody against a natural antigen and a monoclonal antibody that was prepared by natural protein as antigen and can specifically target the GSAEPs. The kit shows the ability to accurately identify venoms from similar genera of Trimeresurus and Protobothrops with a detection limit of 6.25 ng/ml on the snake venoms and a little cross-reaction, thus proving high feasibility and applicability. |
| format |
article |
| author |
Chengbo Long Chengbo Long Feilong Wu Feilong Wu Qiumin Lu Qiumin Lu Bing Xie Chuanbin Shen Jiayao Li Yanling Deng Ping Liang Yongzhi Yu Ren Lai Ren Lai |
| author_facet |
Chengbo Long Chengbo Long Feilong Wu Feilong Wu Qiumin Lu Qiumin Lu Bing Xie Chuanbin Shen Jiayao Li Yanling Deng Ping Liang Yongzhi Yu Ren Lai Ren Lai |
| author_sort |
Chengbo Long |
| title |
A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites |
| title_short |
A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites |
| title_full |
A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites |
| title_fullStr |
A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites |
| title_full_unstemmed |
A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites |
| title_sort |
strategy for efficient preparation of genus-specific diagnostic antibodies for snakebites |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/add56f49384e49e99ccde196caf6f477 |
| work_keys_str_mv |
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1718441324352372736 |