A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites

As said by former United Nations Secretary-General Kofi Annan, “Snakebite is the most important tropical disease you’ve never heard of.” Listed as a priority neglected tropical disease by the World Health Organization, snakebite envenoming (SBE) kills in excess of 125,000 people per year. However, d...

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Autores principales: Chengbo Long, Feilong Wu, Qiumin Lu, Bing Xie, Chuanbin Shen, Jiayao Li, Yanling Deng, Ping Liang, Yongzhi Yu, Ren Lai
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Publicado: Frontiers Media S.A. 2021
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spelling oai:doaj.org-article:add56f49384e49e99ccde196caf6f4772021-11-09T05:37:30ZA Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites1664-322410.3389/fimmu.2021.775678https://doaj.org/article/add56f49384e49e99ccde196caf6f4772021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.775678/fullhttps://doaj.org/toc/1664-3224As said by former United Nations Secretary-General Kofi Annan, “Snakebite is the most important tropical disease you’ve never heard of.” Listed as a priority neglected tropical disease by the World Health Organization, snakebite envenoming (SBE) kills in excess of 125,000 people per year. However, due to the complexity and overlap of snake venom compositions, few reliable venom diagnostic methods for genus-/species-specific identification, which is crucial for successful SBE therapy, are available. Here, we develop a strategy to select and prepare genus-specific snake venom antibodies, which allows rapid and efficient clinical diagnosis of snakebite. Multi-omics approaches are used to choose candidate antigens from snake venoms and identify genus-specific antigenic epitope peptide fragments (GSAEPs) with ideal immunogenicity, specificity, and spatial accessibility. Double-antibody sandwich ELISA kit was established by matching a polyclonal antibody against a natural antigen and a monoclonal antibody that was prepared by natural protein as antigen and can specifically target the GSAEPs. The kit shows the ability to accurately identify venoms from similar genera of Trimeresurus and Protobothrops with a detection limit of 6.25 ng/ml on the snake venoms and a little cross-reaction, thus proving high feasibility and applicability.Chengbo LongChengbo LongFeilong WuFeilong WuQiumin LuQiumin LuBing XieChuanbin ShenJiayao LiYanling DengPing LiangYongzhi YuRen LaiRen LaiFrontiers Media S.A.articlesnakebite envenomingvenomantigenimmunogenicitydiagnosisImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution DOAJ
collection DOAJ
language EN
topic snakebite envenoming
venom
antigen
immunogenicity
diagnosis
Immunologic diseases. Allergy
RC581-607
spellingShingle snakebite envenoming
venom
antigen
immunogenicity
diagnosis
Immunologic diseases. Allergy
RC581-607
Chengbo Long
Chengbo Long
Feilong Wu
Feilong Wu
Qiumin Lu
Qiumin Lu
Bing Xie
Chuanbin Shen
Jiayao Li
Yanling Deng
Ping Liang
Yongzhi Yu
Ren Lai
Ren Lai
A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites
description As said by former United Nations Secretary-General Kofi Annan, “Snakebite is the most important tropical disease you’ve never heard of.” Listed as a priority neglected tropical disease by the World Health Organization, snakebite envenoming (SBE) kills in excess of 125,000 people per year. However, due to the complexity and overlap of snake venom compositions, few reliable venom diagnostic methods for genus-/species-specific identification, which is crucial for successful SBE therapy, are available. Here, we develop a strategy to select and prepare genus-specific snake venom antibodies, which allows rapid and efficient clinical diagnosis of snakebite. Multi-omics approaches are used to choose candidate antigens from snake venoms and identify genus-specific antigenic epitope peptide fragments (GSAEPs) with ideal immunogenicity, specificity, and spatial accessibility. Double-antibody sandwich ELISA kit was established by matching a polyclonal antibody against a natural antigen and a monoclonal antibody that was prepared by natural protein as antigen and can specifically target the GSAEPs. The kit shows the ability to accurately identify venoms from similar genera of Trimeresurus and Protobothrops with a detection limit of 6.25 ng/ml on the snake venoms and a little cross-reaction, thus proving high feasibility and applicability.
format article
author Chengbo Long
Chengbo Long
Feilong Wu
Feilong Wu
Qiumin Lu
Qiumin Lu
Bing Xie
Chuanbin Shen
Jiayao Li
Yanling Deng
Ping Liang
Yongzhi Yu
Ren Lai
Ren Lai
author_facet Chengbo Long
Chengbo Long
Feilong Wu
Feilong Wu
Qiumin Lu
Qiumin Lu
Bing Xie
Chuanbin Shen
Jiayao Li
Yanling Deng
Ping Liang
Yongzhi Yu
Ren Lai
Ren Lai
author_sort Chengbo Long
title A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites
title_short A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites
title_full A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites
title_fullStr A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites
title_full_unstemmed A Strategy for Efficient Preparation of Genus-Specific Diagnostic Antibodies for Snakebites
title_sort strategy for efficient preparation of genus-specific diagnostic antibodies for snakebites
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/add56f49384e49e99ccde196caf6f477
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