Comparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm

Abstract Semen analysis is used for diagnosing male infertility and evaluating male fertility for more than a century. However, the semen analysis simply represents the population characteristics of sperm. It is not a comprehensive assessment of the male reproductive potential. In this study, 20 sem...

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Autores principales:	X. M. Wang, Z. Xiang, Y. Fu, H. L. Wu, W. B. Zhu, L. Q. Fan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ade0ac12f8ad46be81af76d3342dc818
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spelling	oai:doaj.org-article:ade0ac12f8ad46be81af76d3342dc8182021-12-02T12:32:47ZComparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm10.1038/s41598-018-25032-42045-2322https://doaj.org/article/ade0ac12f8ad46be81af76d3342dc8182018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25032-4https://doaj.org/toc/2045-2322Abstract Semen analysis is used for diagnosing male infertility and evaluating male fertility for more than a century. However, the semen analysis simply represents the population characteristics of sperm. It is not a comprehensive assessment of the male reproductive potential. In this study, 20 semen samples from human sperm bank with distinctive artificial insemination with donor sperm (AID) clinical outcomes were collected and analyzed using a two-dimensional differential in-gel electrophoresis (2D-DIGE); 45 differentially expressed protein spots were obtained, and 26 proteins were identified. Most differentially expressed proteins were related to sperm motility, energy consumption, and structure. These identified proteins included several sperm proteins associated with the nucleus on the X chromosome (SPANX) proteins. This prospective study aimed to investigate the association between the expression levels of SPANX proteins and the AID clinical outcomes. The proteins identified in this study provided a reference for the molecular mechanism of sperm fertility and revealed a predictive value of the SPANX proteins.X. M. WangZ. XiangY. FuH. L. WuW. B. ZhuL. Q. FanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q X. M. Wang Z. Xiang Y. Fu H. L. Wu W. B. Zhu L. Q. Fan Comparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm
description	Abstract Semen analysis is used for diagnosing male infertility and evaluating male fertility for more than a century. However, the semen analysis simply represents the population characteristics of sperm. It is not a comprehensive assessment of the male reproductive potential. In this study, 20 semen samples from human sperm bank with distinctive artificial insemination with donor sperm (AID) clinical outcomes were collected and analyzed using a two-dimensional differential in-gel electrophoresis (2D-DIGE); 45 differentially expressed protein spots were obtained, and 26 proteins were identified. Most differentially expressed proteins were related to sperm motility, energy consumption, and structure. These identified proteins included several sperm proteins associated with the nucleus on the X chromosome (SPANX) proteins. This prospective study aimed to investigate the association between the expression levels of SPANX proteins and the AID clinical outcomes. The proteins identified in this study provided a reference for the molecular mechanism of sperm fertility and revealed a predictive value of the SPANX proteins.
format	article
author	X. M. Wang Z. Xiang Y. Fu H. L. Wu W. B. Zhu L. Q. Fan
author_facet	X. M. Wang Z. Xiang Y. Fu H. L. Wu W. B. Zhu L. Q. Fan
author_sort	X. M. Wang
title	Comparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm
title_short	Comparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm
title_full	Comparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm
title_fullStr	Comparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm
title_full_unstemmed	Comparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm
title_sort	comparative proteomics reveal the association between spanx proteins and clinical outcomes of artificial insemination with donor sperm
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ade0ac12f8ad46be81af76d3342dc818
work_keys_str_mv	AT xmwang comparativeproteomicsrevealtheassociationbetweenspanxproteinsandclinicaloutcomesofartificialinseminationwithdonorsperm AT zxiang comparativeproteomicsrevealtheassociationbetweenspanxproteinsandclinicaloutcomesofartificialinseminationwithdonorsperm AT yfu comparativeproteomicsrevealtheassociationbetweenspanxproteinsandclinicaloutcomesofartificialinseminationwithdonorsperm AT hlwu comparativeproteomicsrevealtheassociationbetweenspanxproteinsandclinicaloutcomesofartificialinseminationwithdonorsperm AT wbzhu comparativeproteomicsrevealtheassociationbetweenspanxproteinsandclinicaloutcomesofartificialinseminationwithdonorsperm AT lqfan comparativeproteomicsrevealtheassociationbetweenspanxproteinsandclinicaloutcomesofartificialinseminationwithdonorsperm
_version_	1718393999833694208

Comparative Proteomics Reveal the Association between SPANX Proteins and Clinical Outcomes of Artificial Insemination with Donor Sperm

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