Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity

Porcine reproductive and respiratory syndrome is the most economically important viral disease in the swine industry worldwide. Porcine reproductive and respiratory syndrome virus (PRRSV) strains are classified into two distinct genotypes, the European genotype and the North American genotype. The E...

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Autores principales:	Karniychuk U Uladzimir, Nauwynck J Hans
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2014
Materias:	porcine reproductive and respiratory syndrome virus serology diagnosis belarus Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/ade168db87f24fddbd92230b3264b0bc
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spelling	oai:doaj.org-article:ade168db87f24fddbd92230b3264b0bc2021-11-17T21:27:52ZPerformance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity1820-744810.2478/acve-2014-0005https://doaj.org/article/ade168db87f24fddbd92230b3264b0bc2014-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2014-0005https://doaj.org/toc/1820-7448Porcine reproductive and respiratory syndrome is the most economically important viral disease in the swine industry worldwide. Porcine reproductive and respiratory syndrome virus (PRRSV) strains are classified into two distinct genotypes, the European genotype and the North American genotype. The European PRRSV genotype has been divided into three subtypes: a pan-European subtype 1 and East European subtypes 2 and 3. The aim of this study was to evaluate the performance of commercial and homemade serological assays to test field sera from a geographical region with an extreme PRRSV heterogeneity. Belarus became the country of choice for sample collection because heterologous PRRSV strains of all known European subtypes circulate in this country. Sera from Belarusian swine farms were tested in immunoperoxidase monolayer assays based on pan-European subtype 1, East European subtype 3 and North American strains as antigens and commercial enzyme-linked immunosorbent assays (IDEXX and INGEZIM). The obtained results suggest that none of the serological tools for PRRSV diagnosis can guarantee a flawless detection of antibodies at the individual animal level. Considering heterogeneity of recently isolated European PRRSV strains the problem can be relevant in many countries.Karniychuk U UladzimirNauwynck J HansSciendoarticleporcine reproductive and respiratory syndrome virusserologydiagnosisbelarusVeterinary medicineSF600-1100ENActa Veterinaria, Vol 64, Iss 1, Pp 44-51 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	porcine reproductive and respiratory syndrome virus serology diagnosis belarus Veterinary medicine SF600-1100
spellingShingle	porcine reproductive and respiratory syndrome virus serology diagnosis belarus Veterinary medicine SF600-1100 Karniychuk U Uladzimir Nauwynck J Hans Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity
description	Porcine reproductive and respiratory syndrome is the most economically important viral disease in the swine industry worldwide. Porcine reproductive and respiratory syndrome virus (PRRSV) strains are classified into two distinct genotypes, the European genotype and the North American genotype. The European PRRSV genotype has been divided into three subtypes: a pan-European subtype 1 and East European subtypes 2 and 3. The aim of this study was to evaluate the performance of commercial and homemade serological assays to test field sera from a geographical region with an extreme PRRSV heterogeneity. Belarus became the country of choice for sample collection because heterologous PRRSV strains of all known European subtypes circulate in this country. Sera from Belarusian swine farms were tested in immunoperoxidase monolayer assays based on pan-European subtype 1, East European subtype 3 and North American strains as antigens and commercial enzyme-linked immunosorbent assays (IDEXX and INGEZIM). The obtained results suggest that none of the serological tools for PRRSV diagnosis can guarantee a flawless detection of antibodies at the individual animal level. Considering heterogeneity of recently isolated European PRRSV strains the problem can be relevant in many countries.
format	article
author	Karniychuk U Uladzimir Nauwynck J Hans
author_facet	Karniychuk U Uladzimir Nauwynck J Hans
author_sort	Karniychuk U Uladzimir
title	Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity
title_short	Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity
title_full	Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity
title_fullStr	Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity
title_full_unstemmed	Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity
title_sort	performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity
publisher	Sciendo
publishDate	2014
url	https://doaj.org/article/ade168db87f24fddbd92230b3264b0bc
work_keys_str_mv	AT karniychukuuladzimir performanceofassaysfortestingantibodiesagainstporcinereproductiveandrespiratorysyndromevirusinseracollectedfromswinefarmsinaregionwithanextremevirusheterogeneity AT nauwynckjhans performanceofassaysfortestingantibodiesagainstporcinereproductiveandrespiratorysyndromevirusinseracollectedfromswinefarmsinaregionwithanextremevirusheterogeneity
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Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity

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