Meniscus regeneration with injectable Pluronic/PMMA-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model

Injectable hydrogel systems are a facile approach to apply to the damaged meniscus in a minimally invasive way. We herein developed a clinically applicable and injectable semi-interpenetrated network (semi-IPN) hydrogel system based on fibrin (Fb), reinforced with Pluronic F127 (F127) and polymethyl...

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Autores principales:	Young-Hyeon An, Jin-A Kim, Hyun-Gu Yim, Woo-Jung Han, Yong-Beom Park, Hyun Jin Park, Man Young Kim, Jaewon Jang, Racheal H. Koh, Su-Hwan Kim, Nathaniel S. Hwang, Chul-Won Ha
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/ade7a4ba1dde4d5f9b3c31ea84afa284
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spelling	oai:doaj.org-article:ade7a4ba1dde4d5f9b3c31ea84afa2842021-11-23T11:33:19ZMeniscus regeneration with injectable Pluronic/PMMA-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model2041-731410.1177/20417314211050141https://doaj.org/article/ade7a4ba1dde4d5f9b3c31ea84afa2842021-10-01T00:00:00Zhttps://doi.org/10.1177/20417314211050141https://doaj.org/toc/2041-7314Injectable hydrogel systems are a facile approach to apply to the damaged meniscus in a minimally invasive way. We herein developed a clinically applicable and injectable semi-interpenetrated network (semi-IPN) hydrogel system based on fibrin (Fb), reinforced with Pluronic F127 (F127) and polymethyl methacrylate (PMMA), to improve the intrinsic weak mechanical properties. Through the dual-syringe device system, the hydrogel could form a gel state within about 50 s, and the increment of compressive modulus of Fb hydrogels was achieved by adding F127 from 3.0% (72.0 ± 4.3 kPa) to 10.0% (156.0 ± 9.8 kPa). The shear modulus was enhanced by adding PMMA microbeads (26.0 ± 1.1 kPa), which was higher than Fb (13.5 ± 0.5 kPa) and Fb/F127 (21.7 ± 0.8 kPa). Moreover, the addition of F127 and PMMA also delayed the rate of enzymatic biodegradation of Fb hydrogel. Finally, we confirmed that both Fb/F127 and Fb/F127/PMMA hydrogels showed accelerated tissue repair in the in vivo segmental defect of the rabbit meniscus model. In addition, the histological analysis showed that the quality of the regenerated tissues healed by Fb/F127 was particularly comparable to that of healthy tissue. The biomechanical strength of the regenerated tissues of Fb/F127 (3.50 ± 0.35 MPa) and Fb/F127/PMMA (3.59 ± 0.89 MPa) was much higher than that of Fb (0.82 ± 0.05 MPa) but inferior to that of healthy tissue (6.63 ± 1.12 MPa). These results suggest that the reinforcement of Fb hydrogel using FDA-approved synthetic biomaterials has great potential to be used clinically.Young-Hyeon AnJin-A KimHyun-Gu YimWoo-Jung HanYong-Beom ParkHyun Jin ParkMan Young KimJaewon JangRacheal H. KohSu-Hwan KimNathaniel S. HwangChul-Won HaSAGE PublishingarticleBiochemistryQD415-436ENJournal of Tissue Engineering, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biochemistry QD415-436
spellingShingle	Biochemistry QD415-436 Young-Hyeon An Jin-A Kim Hyun-Gu Yim Woo-Jung Han Yong-Beom Park Hyun Jin Park Man Young Kim Jaewon Jang Racheal H. Koh Su-Hwan Kim Nathaniel S. Hwang Chul-Won Ha Meniscus regeneration with injectable Pluronic/PMMA-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model
description	Injectable hydrogel systems are a facile approach to apply to the damaged meniscus in a minimally invasive way. We herein developed a clinically applicable and injectable semi-interpenetrated network (semi-IPN) hydrogel system based on fibrin (Fb), reinforced with Pluronic F127 (F127) and polymethyl methacrylate (PMMA), to improve the intrinsic weak mechanical properties. Through the dual-syringe device system, the hydrogel could form a gel state within about 50 s, and the increment of compressive modulus of Fb hydrogels was achieved by adding F127 from 3.0% (72.0 ± 4.3 kPa) to 10.0% (156.0 ± 9.8 kPa). The shear modulus was enhanced by adding PMMA microbeads (26.0 ± 1.1 kPa), which was higher than Fb (13.5 ± 0.5 kPa) and Fb/F127 (21.7 ± 0.8 kPa). Moreover, the addition of F127 and PMMA also delayed the rate of enzymatic biodegradation of Fb hydrogel. Finally, we confirmed that both Fb/F127 and Fb/F127/PMMA hydrogels showed accelerated tissue repair in the in vivo segmental defect of the rabbit meniscus model. In addition, the histological analysis showed that the quality of the regenerated tissues healed by Fb/F127 was particularly comparable to that of healthy tissue. The biomechanical strength of the regenerated tissues of Fb/F127 (3.50 ± 0.35 MPa) and Fb/F127/PMMA (3.59 ± 0.89 MPa) was much higher than that of Fb (0.82 ± 0.05 MPa) but inferior to that of healthy tissue (6.63 ± 1.12 MPa). These results suggest that the reinforcement of Fb hydrogel using FDA-approved synthetic biomaterials has great potential to be used clinically.
format	article
author	Young-Hyeon An Jin-A Kim Hyun-Gu Yim Woo-Jung Han Yong-Beom Park Hyun Jin Park Man Young Kim Jaewon Jang Racheal H. Koh Su-Hwan Kim Nathaniel S. Hwang Chul-Won Ha
author_facet	Young-Hyeon An Jin-A Kim Hyun-Gu Yim Woo-Jung Han Yong-Beom Park Hyun Jin Park Man Young Kim Jaewon Jang Racheal H. Koh Su-Hwan Kim Nathaniel S. Hwang Chul-Won Ha
author_sort	Young-Hyeon An
title	Meniscus regeneration with injectable Pluronic/PMMA-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model
title_short	Meniscus regeneration with injectable Pluronic/PMMA-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model
title_full	Meniscus regeneration with injectable Pluronic/PMMA-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model
title_fullStr	Meniscus regeneration with injectable Pluronic/PMMA-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model
title_full_unstemmed	Meniscus regeneration with injectable Pluronic/PMMA-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model
title_sort	meniscus regeneration with injectable pluronic/pmma-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/ade7a4ba1dde4d5f9b3c31ea84afa284
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Meniscus regeneration with injectable Pluronic/PMMA-reinforced fibrin hydrogels in a rabbit segmental meniscectomy model

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