Cross-linked polyethylenimine–tripolyphosphate nanoparticles for gene delivery

Cross-linked polyethylenimine–tripolyphosphate nanoparticles for gene delivery

Xianzhang Huang,1 Sujing Shen,2 Zhanfeng Zhang,1 Junhua Zhuang1 1Department of Laboratory Science, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2Department of Laboratory Science, Guangdong Second Provincial Traditional Chinese Medicine Hospital, Guangzhou, People&rsq...

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Autores principales: Huang XZ, Shen SJ, Zhang ZF, Zhuang JH
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2014
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/adfee0249bb94db98a1dceec51c353e4
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spelling oai:doaj.org-article:adfee0249bb94db98a1dceec51c353e42021-12-02T08:20:23ZCross-linked polyethylenimine–tripolyphosphate nanoparticles for gene delivery1178-2013https://doaj.org/article/adfee0249bb94db98a1dceec51c353e42014-10-01T00:00:00Zhttp://www.dovepress.com/cross-linked-polyethyleniminendashtripolyphosphate-nanoparticles-for-g-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Xianzhang Huang,1 Sujing Shen,2 Zhanfeng Zhang,1 Junhua Zhuang1 1Department of Laboratory Science, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2Department of Laboratory Science, Guangdong Second Provincial Traditional Chinese Medicine Hospital, Guangzhou, People’s Republic of China Abstract: The high transfection efficiency of polyethylenimine (PEI) makes it an attractive potential nonviral genetic vector for gene delivery and therapy. However, the highly positive charge of PEI leads to cytotoxicity and limits its application. To reduce the cytotoxicity of PEI, we prepared anion-enriched nanoparticles that combined PEI with tripolyphosphate (TPP). We then characterized the PEI-TPP nanoparticles in terms of size, zeta potential, and Fourier-transform infrared (FTIR) spectra, and assessed their transfection efficiency, cytotoxicity, and ability to resist deoxyribonuclease (DNase) I digestion. The cellular uptake of PEI-TPP with phosphorylated internal ribosome entry site–enhanced green fluorescent protein C1 or FAM (fluorouracil, Adriamycin [doxorubicin] and mitomycin)-labeled small interfering ribonucleic acids (siRNAs) was monitored by fluorescence microscopy and confocal laser microscopy. The efficiency of transfected delivery of plasmid deoxyribonucleic acid (DNA) and siRNA in vitro was 1.11- to 4.20-fold higher with the PEI-TPP particles (7.6% cross-linked) than with the PEI, at all N:P ratios (nitrogen in PEI to phosphorus in DNA) tested. The cell viability of different cell lines was more than 90% at the chosen N:P ratios of PEI-TPP/DNA complexes. Moreover, PEI-TPP nanoparticles resisted digestion by DNase I for more than 2 hours. The time-dependent absorption experiment showed that 7.6% of cross-linked PEI-TPP particles were internalized by 293T cells within 1 hour. In summary, PEI-TPP nanoparticles effectively transfected cells while conferring little or no toxicity, and thus have potential application in gene delivery. Keywords: polyethylenimine (PEI), tripolyphosphate (TPP), nanoparticles (NPs), transfectionHuang XZShen SJZhang ZFZhuang JHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 4785-4794 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Huang XZ
Shen SJ
Zhang ZF
Zhuang JH
Cross-linked polyethylenimine–tripolyphosphate nanoparticles for gene delivery
description Xianzhang Huang,1 Sujing Shen,2 Zhanfeng Zhang,1 Junhua Zhuang1 1Department of Laboratory Science, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2Department of Laboratory Science, Guangdong Second Provincial Traditional Chinese Medicine Hospital, Guangzhou, People’s Republic of China Abstract: The high transfection efficiency of polyethylenimine (PEI) makes it an attractive potential nonviral genetic vector for gene delivery and therapy. However, the highly positive charge of PEI leads to cytotoxicity and limits its application. To reduce the cytotoxicity of PEI, we prepared anion-enriched nanoparticles that combined PEI with tripolyphosphate (TPP). We then characterized the PEI-TPP nanoparticles in terms of size, zeta potential, and Fourier-transform infrared (FTIR) spectra, and assessed their transfection efficiency, cytotoxicity, and ability to resist deoxyribonuclease (DNase) I digestion. The cellular uptake of PEI-TPP with phosphorylated internal ribosome entry site–enhanced green fluorescent protein C1 or FAM (fluorouracil, Adriamycin [doxorubicin] and mitomycin)-labeled small interfering ribonucleic acids (siRNAs) was monitored by fluorescence microscopy and confocal laser microscopy. The efficiency of transfected delivery of plasmid deoxyribonucleic acid (DNA) and siRNA in vitro was 1.11- to 4.20-fold higher with the PEI-TPP particles (7.6% cross-linked) than with the PEI, at all N:P ratios (nitrogen in PEI to phosphorus in DNA) tested. The cell viability of different cell lines was more than 90% at the chosen N:P ratios of PEI-TPP/DNA complexes. Moreover, PEI-TPP nanoparticles resisted digestion by DNase I for more than 2 hours. The time-dependent absorption experiment showed that 7.6% of cross-linked PEI-TPP particles were internalized by 293T cells within 1 hour. In summary, PEI-TPP nanoparticles effectively transfected cells while conferring little or no toxicity, and thus have potential application in gene delivery. Keywords: polyethylenimine (PEI), tripolyphosphate (TPP), nanoparticles (NPs), transfection
format article
author Huang XZ
Shen SJ
Zhang ZF
Zhuang JH
author_facet Huang XZ
Shen SJ
Zhang ZF
Zhuang JH
author_sort Huang XZ
title Cross-linked polyethylenimine–tripolyphosphate nanoparticles for gene delivery
title_short Cross-linked polyethylenimine–tripolyphosphate nanoparticles for gene delivery
title_full Cross-linked polyethylenimine–tripolyphosphate nanoparticles for gene delivery
title_fullStr Cross-linked polyethylenimine–tripolyphosphate nanoparticles for gene delivery
title_full_unstemmed Cross-linked polyethylenimine–tripolyphosphate nanoparticles for gene delivery
title_sort cross-linked polyethylenimine–tripolyphosphate nanoparticles for gene delivery
publisher Dove Medical Press
publishDate 2014
url https://doaj.org/article/adfee0249bb94db98a1dceec51c353e4
work_keys_str_mv AT huangxz crosslinkedpolyethyleniminendashtripolyphosphatenanoparticlesforgenedelivery
AT shensj crosslinkedpolyethyleniminendashtripolyphosphatenanoparticlesforgenedelivery
AT zhangzf crosslinkedpolyethyleniminendashtripolyphosphatenanoparticlesforgenedelivery
AT zhuangjh crosslinkedpolyethyleniminendashtripolyphosphatenanoparticlesforgenedelivery
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