Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China

Abstract Encephalomyocarditis virus (EMCV) is as a potential zoonotic agent with a wide host range. Here, we describe an EMC virus isolate, identified as EMCV C15, which was successfully obtained from the serum of dogs from animal hospitals. Virus production in cell culture was confirmed by EMCV-spe...

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Autores principales:	Ya-Kun Luo, Lin Liang, Qing-Hai Tang, Ling Zhou, Li-Jun Shi, Ying-Ying Cong, Wen-Cheng Lin, Shang-Jin Cui
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/adffb5ff28ab447ca237c6eb460d91eb
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spelling	oai:doaj.org-article:adffb5ff28ab447ca237c6eb460d91eb2021-12-02T15:05:33ZIsolation and Characterization of Encephalomyocarditis Virus from Dogs in China10.1038/s41598-017-00435-x2045-2322https://doaj.org/article/adffb5ff28ab447ca237c6eb460d91eb2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00435-xhttps://doaj.org/toc/2045-2322Abstract Encephalomyocarditis virus (EMCV) is as a potential zoonotic agent with a wide host range. Here, we describe an EMC virus isolate, identified as EMCV C15, which was successfully obtained from the serum of dogs from animal hospitals. Virus production in cell culture was confirmed by EMCV-specific real-time RT-PCR, indirect immunofluorescence assays and electron microscopy. In addition, the open reading frame sequence (ORF) of the EMCV C15 virus was determined. From sequence comparison and phylogenetic analysis among 24 reference EMCV strains, it appears that the EMCV C15 strain is closely genetically related to strain BEL2887A/91 (>99.0% nucleotide identity). In artificially challenged dogs, the heart and brain were important targets of EMCV C15. This study provides genetic and pathogenic characterization of the EMCV C15 strain isolated in Beijing and calls for sustained surveillance of EMCV infection in China to support better prevention and control of the disease.Ya-Kun LuoLin LiangQing-Hai TangLing ZhouLi-Jun ShiYing-Ying CongWen-Cheng LinShang-Jin CuiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ya-Kun Luo Lin Liang Qing-Hai Tang Ling Zhou Li-Jun Shi Ying-Ying Cong Wen-Cheng Lin Shang-Jin Cui Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China
description	Abstract Encephalomyocarditis virus (EMCV) is as a potential zoonotic agent with a wide host range. Here, we describe an EMC virus isolate, identified as EMCV C15, which was successfully obtained from the serum of dogs from animal hospitals. Virus production in cell culture was confirmed by EMCV-specific real-time RT-PCR, indirect immunofluorescence assays and electron microscopy. In addition, the open reading frame sequence (ORF) of the EMCV C15 virus was determined. From sequence comparison and phylogenetic analysis among 24 reference EMCV strains, it appears that the EMCV C15 strain is closely genetically related to strain BEL2887A/91 (>99.0% nucleotide identity). In artificially challenged dogs, the heart and brain were important targets of EMCV C15. This study provides genetic and pathogenic characterization of the EMCV C15 strain isolated in Beijing and calls for sustained surveillance of EMCV infection in China to support better prevention and control of the disease.
format	article
author	Ya-Kun Luo Lin Liang Qing-Hai Tang Ling Zhou Li-Jun Shi Ying-Ying Cong Wen-Cheng Lin Shang-Jin Cui
author_facet	Ya-Kun Luo Lin Liang Qing-Hai Tang Ling Zhou Li-Jun Shi Ying-Ying Cong Wen-Cheng Lin Shang-Jin Cui
author_sort	Ya-Kun Luo
title	Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China
title_short	Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China
title_full	Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China
title_fullStr	Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China
title_full_unstemmed	Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China
title_sort	isolation and characterization of encephalomyocarditis virus from dogs in china
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/adffb5ff28ab447ca237c6eb460d91eb
work_keys_str_mv	AT yakunluo isolationandcharacterizationofencephalomyocarditisvirusfromdogsinchina AT linliang isolationandcharacterizationofencephalomyocarditisvirusfromdogsinchina AT qinghaitang isolationandcharacterizationofencephalomyocarditisvirusfromdogsinchina AT lingzhou isolationandcharacterizationofencephalomyocarditisvirusfromdogsinchina AT lijunshi isolationandcharacterizationofencephalomyocarditisvirusfromdogsinchina AT yingyingcong isolationandcharacterizationofencephalomyocarditisvirusfromdogsinchina AT wenchenglin isolationandcharacterizationofencephalomyocarditisvirusfromdogsinchina AT shangjincui isolationandcharacterizationofencephalomyocarditisvirusfromdogsinchina
_version_	1718388787174703104

Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China

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