Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection

Abstract. Yunita R, Dewi IS, Lestari EG, Purnamanengsih R, Rahayu S, Mastur. 2020. Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection. Biodiversitas 21: 1476-1482. Increased production can be done by increasing the planting area, but the available land is...

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Autores principales:	Rossa Yunita, ISWARI S. DEWI, ENDANG GATI LESTARI, RAGAPADMI PURNAMANENGSIH, SUCI RAHAYU, MASTUR
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	batutegi, gamma irradiation, ld 50% value of nacl, oryza sativa, situpatenggang Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/adffb8d62ea34c2bb851ae59a490eec6
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spelling	oai:doaj.org-article:adffb8d62ea34c2bb851ae59a490eec62021-11-22T00:34:40ZFormation of upland rice drought-tolerant mutants by mutation induction and in vitro selection1412-033X2085-472210.13057/biodiv/d210427https://doaj.org/article/adffb8d62ea34c2bb851ae59a490eec62020-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5292https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Yunita R, Dewi IS, Lestari EG, Purnamanengsih R, Rahayu S, Mastur. 2020. Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection. Biodiversitas 21: 1476-1482. Increased production can be done by increasing the planting area, but the available land is sub-optimal land with drought stress. Drought-tolerant rice varieties are needed to utilize this land. To create drought-tolerant rice can use induction mutation and in vitro selection methods. This study aims to obtain tolerant upland rice mutants through mutation and in vitro selection. The parents used were Batutegi and Situpatenggang varieties. This research consisted of several main activities, namely callus induction, determination of LD 50% value ofPEG determination of LC 50% value of gamma-ray irradiation, mutation induction and in vitro selection, bud regeneration and acclimatization. The results of this study are mutant callus formed by gamma-ray irradiation at a dose of 24.68 Gy for Situpatenggang and 22.15 Gy for Batutegi (LD50) and selected on PEG media at a dose of 24.11% for Situpatenggang and 25.18% for Batutegi (LC50). The mutant callus regenerated on MS + BA 3 mg/L + Zeatin 0.1 mg/L produced 83 Situpatenggang shoots and 73 Batutegi shoots and successfully acclimatized were 52 Situpatenggang lines and 49 Batuteg linesBatutegiRossa YunitaISWARI S. DEWIENDANG GATI LESTARIRAGAPADMI PURNAMANENGSIHSUCI RAHAYUMASTURMBI & UNS Soloarticlebatutegi, gamma irradiation, ld 50% value of nacl, oryza sativa, situpatenggangBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	batutegi, gamma irradiation, ld 50% value of nacl, oryza sativa, situpatenggang Biology (General) QH301-705.5
spellingShingle	batutegi, gamma irradiation, ld 50% value of nacl, oryza sativa, situpatenggang Biology (General) QH301-705.5 Rossa Yunita ISWARI S. DEWI ENDANG GATI LESTARI RAGAPADMI PURNAMANENGSIH SUCI RAHAYU MASTUR Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection
description	Abstract. Yunita R, Dewi IS, Lestari EG, Purnamanengsih R, Rahayu S, Mastur. 2020. Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection. Biodiversitas 21: 1476-1482. Increased production can be done by increasing the planting area, but the available land is sub-optimal land with drought stress. Drought-tolerant rice varieties are needed to utilize this land. To create drought-tolerant rice can use induction mutation and in vitro selection methods. This study aims to obtain tolerant upland rice mutants through mutation and in vitro selection. The parents used were Batutegi and Situpatenggang varieties. This research consisted of several main activities, namely callus induction, determination of LD 50% value ofPEG determination of LC 50% value of gamma-ray irradiation, mutation induction and in vitro selection, bud regeneration and acclimatization. The results of this study are mutant callus formed by gamma-ray irradiation at a dose of 24.68 Gy for Situpatenggang and 22.15 Gy for Batutegi (LD50) and selected on PEG media at a dose of 24.11% for Situpatenggang and 25.18% for Batutegi (LC50). The mutant callus regenerated on MS + BA 3 mg/L + Zeatin 0.1 mg/L produced 83 Situpatenggang shoots and 73 Batutegi shoots and successfully acclimatized were 52 Situpatenggang lines and 49 Batuteg linesBatutegi
format	article
author	Rossa Yunita ISWARI S. DEWI ENDANG GATI LESTARI RAGAPADMI PURNAMANENGSIH SUCI RAHAYU MASTUR
author_facet	Rossa Yunita ISWARI S. DEWI ENDANG GATI LESTARI RAGAPADMI PURNAMANENGSIH SUCI RAHAYU MASTUR
author_sort	Rossa Yunita
title	Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection
title_short	Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection
title_full	Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection
title_fullStr	Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection
title_full_unstemmed	Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection
title_sort	formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/adffb8d62ea34c2bb851ae59a490eec6
work_keys_str_mv	AT rossayunita formationofuplandricedroughttolerantmutantsbymutationinductionandinvitroselection AT iswarisdewi formationofuplandricedroughttolerantmutantsbymutationinductionandinvitroselection AT endanggatilestari formationofuplandricedroughttolerantmutantsbymutationinductionandinvitroselection AT ragapadmipurnamanengsih formationofuplandricedroughttolerantmutantsbymutationinductionandinvitroselection AT sucirahayu formationofuplandricedroughttolerantmutantsbymutationinductionandinvitroselection AT mastur formationofuplandricedroughttolerantmutantsbymutationinductionandinvitroselection
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Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection

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