Loss of putzig in the germline impedes germ cell development by inducing cell death and new niche like microenvironments

Abstract Germline stem cell development and differentiation is tightly controlled by the surrounding somatic cells of the stem cell niche. In Drosophila females, cells of the niche emit various signals including Dpp and Wg to balance stem cell renewal and differentiation. Here, we show that the gene...

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Autores principales:	Ludmilla Kober, Mirjam Zimmermann, Michaela Kurz, Melanie Bayer, Anja C. Nagel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ae068ff87b3148bca4ff1bff0019aac6
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spelling	oai:doaj.org-article:ae068ff87b3148bca4ff1bff0019aac62021-12-02T15:08:19ZLoss of putzig in the germline impedes germ cell development by inducing cell death and new niche like microenvironments10.1038/s41598-019-45655-52045-2322https://doaj.org/article/ae068ff87b3148bca4ff1bff0019aac62019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45655-5https://doaj.org/toc/2045-2322Abstract Germline stem cell development and differentiation is tightly controlled by the surrounding somatic cells of the stem cell niche. In Drosophila females, cells of the niche emit various signals including Dpp and Wg to balance stem cell renewal and differentiation. Here, we show that the gene pzg is autonomously required in cells of the germline to sustain the interplay between niche and stem cells. Loss of pzg impairs stem cell differentiation and provokes the death of cells in the germarium. As a consequence of pzg loss, increased growth signalling activity predominantly of Dpp and Wg/Wnt, was observed, eventually disrupting the balance of germ cell self-renewal and differentiation. Whereas in the soma, apoptosis-induced compensatory growth is well established, the induction of self-renewal signals during oogenesis cannot compensate for dying germ cells, albeit inducing a new niche-like microenvironment. Instead, they impair the further development of germ cells and cause in addition a forward and feedback loop of cell death.Ludmilla KoberMirjam ZimmermannMichaela KurzMelanie BayerAnja C. NagelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ludmilla Kober Mirjam Zimmermann Michaela Kurz Melanie Bayer Anja C. Nagel Loss of putzig in the germline impedes germ cell development by inducing cell death and new niche like microenvironments
description	Abstract Germline stem cell development and differentiation is tightly controlled by the surrounding somatic cells of the stem cell niche. In Drosophila females, cells of the niche emit various signals including Dpp and Wg to balance stem cell renewal and differentiation. Here, we show that the gene pzg is autonomously required in cells of the germline to sustain the interplay between niche and stem cells. Loss of pzg impairs stem cell differentiation and provokes the death of cells in the germarium. As a consequence of pzg loss, increased growth signalling activity predominantly of Dpp and Wg/Wnt, was observed, eventually disrupting the balance of germ cell self-renewal and differentiation. Whereas in the soma, apoptosis-induced compensatory growth is well established, the induction of self-renewal signals during oogenesis cannot compensate for dying germ cells, albeit inducing a new niche-like microenvironment. Instead, they impair the further development of germ cells and cause in addition a forward and feedback loop of cell death.
format	article
author	Ludmilla Kober Mirjam Zimmermann Michaela Kurz Melanie Bayer Anja C. Nagel
author_facet	Ludmilla Kober Mirjam Zimmermann Michaela Kurz Melanie Bayer Anja C. Nagel
author_sort	Ludmilla Kober
title	Loss of putzig in the germline impedes germ cell development by inducing cell death and new niche like microenvironments
title_short	Loss of putzig in the germline impedes germ cell development by inducing cell death and new niche like microenvironments
title_full	Loss of putzig in the germline impedes germ cell development by inducing cell death and new niche like microenvironments
title_fullStr	Loss of putzig in the germline impedes germ cell development by inducing cell death and new niche like microenvironments
title_full_unstemmed	Loss of putzig in the germline impedes germ cell development by inducing cell death and new niche like microenvironments
title_sort	loss of putzig in the germline impedes germ cell development by inducing cell death and new niche like microenvironments
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ae068ff87b3148bca4ff1bff0019aac6
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Loss of putzig in the germline impedes germ cell development by inducing cell death and new niche like microenvironments

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