Deltonin Ameliorates Cerebral Ischemia/Reperfusion Injury in Correlation with Modulation of Autophagy and Inflammation

Yi Zhang,1 Zhiming Tian,1 Hongyan Wan,1 Wen Liu,1 Fanping Kong,2 Guoping Ma3 1Cerebral Vascular Center, Zhongda Hospital, Southeast University, Nanjing City, Jiangsu Province 210044, People’s Republic of China; 2Department of Neurology, Fu-Ning People’s Hospital, Yancheng City, J...

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Autores principales:	Zhang Y, Tian Z, Wan H, Liu W, Kong F, Ma G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	deltonin cerebral i/r mcao/r autophagy inflammation Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/ae071de1f4804cb9a73e2c5f64c30127
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spelling	oai:doaj.org-article:ae071de1f4804cb9a73e2c5f64c301272021-12-02T09:52:59ZDeltonin Ameliorates Cerebral Ischemia/Reperfusion Injury in Correlation with Modulation of Autophagy and Inflammation1178-2021https://doaj.org/article/ae071de1f4804cb9a73e2c5f64c301272020-03-01T00:00:00Zhttps://www.dovepress.com/deltonin-ameliorates-cerebral-ischemiareperfusion-injury-in-correlatio-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Yi Zhang,1 Zhiming Tian,1 Hongyan Wan,1 Wen Liu,1 Fanping Kong,2 Guoping Ma3 1Cerebral Vascular Center, Zhongda Hospital, Southeast University, Nanjing City, Jiangsu Province 210044, People’s Republic of China; 2Department of Neurology, Fu-Ning People’s Hospital, Yancheng City, Jiangsu Province 224400, People’s Republic of China; 3Department of Neurology, Tian-Shui First People’s Hospital, Tianshui City, Gansu Province 741000, People’s Republic of ChinaCorrespondence: Fanping KongDepartment of Neurology, Fu-Ning People’s Hospital, No. 111 Bucheng Street, Yancheng City, Jiangsu Province 224400, People’s Republic of ChinaEmail kongfanping_07@163.comGuoping MaDepartment of Neurology, Tian-Shui First People’s Hospital, No. 105 Jianshe Road, Tianshui City, Gansu Province 741000, People’s Republic of ChinaEmail ahygmcynbiis9@163.comIntroduction: Deltonin, an active component extracted from Dioscorea zingiberensis C.H. WRIGHT, was widely utilized in traditional Chinese medicines. It has been shown to have anti-cancer functions such as colon cancer, breast cancer, and head and neck squamous carcinoma. Herein, we will investigate the role of deltonin in cerebral ischemia/reperfusion injuries.Methods: Ly294002 and anisomycin were used as inhibitors to monitor the effects of deltonin. Middle cerebral artery occlusion I/R model was constructed. Infarct volumes, neurological deficits and brain water contents were evaluated under different conditions. Rotarod test, ELISA, and Western blotting were carried to investigate the effects in vitro.Results: We found that deltonin in ischemia/reperfusion (I/R) rats greatly enhanced brain damages as well as neurological functions through up-regulating p-Akt and p-mTOR as well as inhibiting the expressions of LC3-II/LC3-I, Beclin-1, IL-1, TLR4, and p-p38. Deltonin exerted neuroprotection effect through relieving autophagy activity by regulating PI3K/Akt/mTOR signaling. Deltonin suppressed inflammation reactions through modulation TLR4/p38/MAPK signaling as well.Conclusion: Overall, our data suggested that deltonin could suppress ischemic brain injury by regulating autophagy and inflammation during I/R. Deltonin can be a potential therapeutic method for patient with I/R.Keywords: deltonin, cerebral I/R, MCAO/R, autophagy, inflammationZhang YTian ZWan HLiu WKong FMa GDove Medical Pressarticledeltonincerebral i/rmcao/rautophagyinflammationNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 16, Pp 871-879 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	deltonin cerebral i/r mcao/r autophagy inflammation Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	deltonin cerebral i/r mcao/r autophagy inflammation Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Zhang Y Tian Z Wan H Liu W Kong F Ma G Deltonin Ameliorates Cerebral Ischemia/Reperfusion Injury in Correlation with Modulation of Autophagy and Inflammation
description	Yi Zhang,1 Zhiming Tian,1 Hongyan Wan,1 Wen Liu,1 Fanping Kong,2 Guoping Ma3 1Cerebral Vascular Center, Zhongda Hospital, Southeast University, Nanjing City, Jiangsu Province 210044, People’s Republic of China; 2Department of Neurology, Fu-Ning People’s Hospital, Yancheng City, Jiangsu Province 224400, People’s Republic of China; 3Department of Neurology, Tian-Shui First People’s Hospital, Tianshui City, Gansu Province 741000, People’s Republic of ChinaCorrespondence: Fanping KongDepartment of Neurology, Fu-Ning People’s Hospital, No. 111 Bucheng Street, Yancheng City, Jiangsu Province 224400, People’s Republic of ChinaEmail kongfanping_07@163.comGuoping MaDepartment of Neurology, Tian-Shui First People’s Hospital, No. 105 Jianshe Road, Tianshui City, Gansu Province 741000, People’s Republic of ChinaEmail ahygmcynbiis9@163.comIntroduction: Deltonin, an active component extracted from Dioscorea zingiberensis C.H. WRIGHT, was widely utilized in traditional Chinese medicines. It has been shown to have anti-cancer functions such as colon cancer, breast cancer, and head and neck squamous carcinoma. Herein, we will investigate the role of deltonin in cerebral ischemia/reperfusion injuries.Methods: Ly294002 and anisomycin were used as inhibitors to monitor the effects of deltonin. Middle cerebral artery occlusion I/R model was constructed. Infarct volumes, neurological deficits and brain water contents were evaluated under different conditions. Rotarod test, ELISA, and Western blotting were carried to investigate the effects in vitro.Results: We found that deltonin in ischemia/reperfusion (I/R) rats greatly enhanced brain damages as well as neurological functions through up-regulating p-Akt and p-mTOR as well as inhibiting the expressions of LC3-II/LC3-I, Beclin-1, IL-1, TLR4, and p-p38. Deltonin exerted neuroprotection effect through relieving autophagy activity by regulating PI3K/Akt/mTOR signaling. Deltonin suppressed inflammation reactions through modulation TLR4/p38/MAPK signaling as well.Conclusion: Overall, our data suggested that deltonin could suppress ischemic brain injury by regulating autophagy and inflammation during I/R. Deltonin can be a potential therapeutic method for patient with I/R.Keywords: deltonin, cerebral I/R, MCAO/R, autophagy, inflammation
format	article
author	Zhang Y Tian Z Wan H Liu W Kong F Ma G
author_facet	Zhang Y Tian Z Wan H Liu W Kong F Ma G
author_sort	Zhang Y
title	Deltonin Ameliorates Cerebral Ischemia/Reperfusion Injury in Correlation with Modulation of Autophagy and Inflammation
title_short	Deltonin Ameliorates Cerebral Ischemia/Reperfusion Injury in Correlation with Modulation of Autophagy and Inflammation
title_full	Deltonin Ameliorates Cerebral Ischemia/Reperfusion Injury in Correlation with Modulation of Autophagy and Inflammation
title_fullStr	Deltonin Ameliorates Cerebral Ischemia/Reperfusion Injury in Correlation with Modulation of Autophagy and Inflammation
title_full_unstemmed	Deltonin Ameliorates Cerebral Ischemia/Reperfusion Injury in Correlation with Modulation of Autophagy and Inflammation
title_sort	deltonin ameliorates cerebral ischemia/reperfusion injury in correlation with modulation of autophagy and inflammation
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/ae071de1f4804cb9a73e2c5f64c30127
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Deltonin Ameliorates Cerebral Ischemia/Reperfusion Injury in Correlation with Modulation of Autophagy and Inflammation

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