Early detection of MDR Mycobacterium tuberculosis mutations in Pakistan

Abstract The result of improper treatment has led to the rise of Multidrug-resistant (MDR) strains. This concern still exists in Pakistan. In order to save energy, time and resources an early detection of resistant cases is imperative. Thus, a treated group of 100 isolates and a control group of 56...

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Autores principales:	Ayma Aftab, Samia Afzal, Zahida Qamar, Muhammad Idrees
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ae0b5e413fae4aacb94f395f886dffab
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spelling	oai:doaj.org-article:ae0b5e413fae4aacb94f395f886dffab2021-12-02T17:08:23ZEarly detection of MDR Mycobacterium tuberculosis mutations in Pakistan10.1038/s41598-021-96116-x2045-2322https://doaj.org/article/ae0b5e413fae4aacb94f395f886dffab2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96116-xhttps://doaj.org/toc/2045-2322Abstract The result of improper treatment has led to the rise of Multidrug-resistant (MDR) strains. This concern still exists in Pakistan. In order to save energy, time and resources an early detection of resistant cases is imperative. Thus, a treated group of 100 isolates and a control group of 56 untreated isolates were studied. PCR and gene sequencing showed mutations at codon 531 and 513 in the rpoB gene. 12% of cases showed a double mutation in the rpoB gene. katG gene showed mutations at codon 315 and 299. 28.6% of the control group cases were positive for MDR whereas 100% of the treated group were positive for MDR. This study explores the significantly increasing ratio of MDR-TB among Pakistani population. This study provides prevalent MDR mutations among Pakistanis and suggests developing such molecular assays that are time and cost effective. Importance: Pakistan is a developing country and has fourth highest incidence rate of MDR-TB. The treatment of MDR-TB is the use of second line drugs that has severe side effects as well as it requires long time span. One of the strategies to control the spread of MDR-TB is to decipher the aberrations at molecular level in order to formulate potent drugs that can treat the patients within short span of time. Determining the mutation profile of MDR in Pakistani populations will open new horizons for the improvement of drug treatment regimens to make it more effective or for the development of novel potent drugs and vaccines to better treat the drug-resistant TB. Moreover, this study will be help in disease control program.Ayma AftabSamia AfzalZahida QamarMuhammad IdreesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ayma Aftab Samia Afzal Zahida Qamar Muhammad Idrees Early detection of MDR Mycobacterium tuberculosis mutations in Pakistan
description	Abstract The result of improper treatment has led to the rise of Multidrug-resistant (MDR) strains. This concern still exists in Pakistan. In order to save energy, time and resources an early detection of resistant cases is imperative. Thus, a treated group of 100 isolates and a control group of 56 untreated isolates were studied. PCR and gene sequencing showed mutations at codon 531 and 513 in the rpoB gene. 12% of cases showed a double mutation in the rpoB gene. katG gene showed mutations at codon 315 and 299. 28.6% of the control group cases were positive for MDR whereas 100% of the treated group were positive for MDR. This study explores the significantly increasing ratio of MDR-TB among Pakistani population. This study provides prevalent MDR mutations among Pakistanis and suggests developing such molecular assays that are time and cost effective. Importance: Pakistan is a developing country and has fourth highest incidence rate of MDR-TB. The treatment of MDR-TB is the use of second line drugs that has severe side effects as well as it requires long time span. One of the strategies to control the spread of MDR-TB is to decipher the aberrations at molecular level in order to formulate potent drugs that can treat the patients within short span of time. Determining the mutation profile of MDR in Pakistani populations will open new horizons for the improvement of drug treatment regimens to make it more effective or for the development of novel potent drugs and vaccines to better treat the drug-resistant TB. Moreover, this study will be help in disease control program.
format	article
author	Ayma Aftab Samia Afzal Zahida Qamar Muhammad Idrees
author_facet	Ayma Aftab Samia Afzal Zahida Qamar Muhammad Idrees
author_sort	Ayma Aftab
title	Early detection of MDR Mycobacterium tuberculosis mutations in Pakistan
title_short	Early detection of MDR Mycobacterium tuberculosis mutations in Pakistan
title_full	Early detection of MDR Mycobacterium tuberculosis mutations in Pakistan
title_fullStr	Early detection of MDR Mycobacterium tuberculosis mutations in Pakistan
title_full_unstemmed	Early detection of MDR Mycobacterium tuberculosis mutations in Pakistan
title_sort	early detection of mdr mycobacterium tuberculosis mutations in pakistan
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ae0b5e413fae4aacb94f395f886dffab
work_keys_str_mv	AT aymaaftab earlydetectionofmdrmycobacteriumtuberculosismutationsinpakistan AT samiaafzal earlydetectionofmdrmycobacteriumtuberculosismutationsinpakistan AT zahidaqamar earlydetectionofmdrmycobacteriumtuberculosismutationsinpakistan AT muhammadidrees earlydetectionofmdrmycobacteriumtuberculosismutationsinpakistan
_version_	1718381575703363584

Early detection of MDR Mycobacterium tuberculosis mutations in Pakistan

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