Electrochemical Deposition of Nanostructured Hydroxyapatite Coating on Titanium with Enhanced Early Stage Osteogenic Activity and Osseointegration

Minxun Lu,1,2 Hongjie Chen,2 Bo Yuan,2 Yong Zhou,1 Li Min,1 Zhanwen Xiao,2 Xiangdong Zhu,2 Chongqi Tu,1 Xingdong Zhang2 1Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, People’s Republic of China; 2National Engineering Research Center for Biomaterials, Sichuan...

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Autores principales:	Lu M, Chen H, Yuan B, Zhou Y, Min L, Xiao Z, Zhu X, Tu C, Zhang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	electrochemical deposition hydroxyapatite coatings nanoscale osteogenic activity osseointegration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ae1f119c681e4eecb778afd0d933f829
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spelling	oai:doaj.org-article:ae1f119c681e4eecb778afd0d933f8292021-12-02T12:34:28ZElectrochemical Deposition of Nanostructured Hydroxyapatite Coating on Titanium with Enhanced Early Stage Osteogenic Activity and Osseointegration1178-2013https://doaj.org/article/ae1f119c681e4eecb778afd0d933f8292020-09-01T00:00:00Zhttps://www.dovepress.com/electrochemical-deposition-of-nanostructured-hydroxyapatite-coating-on-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Minxun Lu,1,2 Hongjie Chen,2 Bo Yuan,2 Yong Zhou,1 Li Min,1 Zhanwen Xiao,2 Xiangdong Zhu,2 Chongqi Tu,1 Xingdong Zhang2 1Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, People’s Republic of China; 2National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, People’s Republic of ChinaCorrespondence: Xiangdong ZhuNational Engineering Research Center for Biomaterials, Sichuan University, No. 29 Wangjiang Road, Chengdu 610064, People’s Republic of ChinaTel +86-28-85470770Email zhu_xd1973@scu.edu.cnChongqi TuDepartment of Orthopedics, West China Hospital, Sichuan University, No. 37 Guoxue Street, Chengdu 610041, People’s Republic of ChinaTel +86-28-85422570Email Tuchongqi@yeah.netPurpose: The aim of research is to fabricate nanostructured hydroxyapatite (HA) coatings on the titanium via electrochemical deposition (ED). Additionally, the biological properties of the ED-produced HA (EDHA) coatings with a plate-like nanostructure were evaluated in vitro and in vivo by undertaking comparisons with those prepared by acid/alkali (AA) treatment and by plasma spray-produced HA (PSHA) nanotopography-free coatings.Materials and Methods: Nanoplate-like HA coatings were prepared through ED, and nanotopography-free PSHA coatings were fabricated. The surface morphology, phase composition, roughness, and wettability of these samples were investigated. Furthermore, the growth, proliferation, and osteogenic differentiation of MC3T3-E1 cells cultured on each sample were evaluated via in vitro experiments. Histological assessment and push-out tests for the bone–implant interface were performed to explore the effect of the EDHA coatings on the interfacial osseointegration in vivo.Results: XRD analysis showed that the strongest intensity for the EDHA coatings was at the (002) plane rather than at the regular (211) plane. Relatively higher surface roughness and greater wettability were observed for the EDHA coatings. Cellular experiments revealed that the plate-like nanostructured EDHA coatings not only possessed an ability, similar to that of PSHA coatings, to promote the adhesion and proliferation of MC3T3-E1 cells but also demonstrated significantly enhanced early or intermediate markers of osteogenic differentiation. Significant osseointegration enhancement in the early stage of implantation period and great bonding strength were observed at the interface of bone and EDHA samples. In comparison, relatively weak osseointegration and bonding strength of the bone–implant interface were observed for the AA treatment.Conclusion: The biological performance of the plate-like nanostructured EDHA coating, which was comparable with that of the PSHA, improves early-stage osteogenic differentiation and osseointegration abilities and has great potential for enhancing the initial stability and long-term survival of uncemented or 3D porous titanium implants.Keywords: electrochemical deposition, hydroxyapatite coatings, nanoscale, osteogenic activity, osseointegrationLu MChen HYuan BZhou YMin LXiao ZZhu XTu CZhang XDove Medical Pressarticleelectrochemical depositionhydroxyapatite coatingsnanoscaleosteogenic activityosseointegrationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 6605-6618 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrochemical deposition hydroxyapatite coatings nanoscale osteogenic activity osseointegration Medicine (General) R5-920
spellingShingle	electrochemical deposition hydroxyapatite coatings nanoscale osteogenic activity osseointegration Medicine (General) R5-920 Lu M Chen H Yuan B Zhou Y Min L Xiao Z Zhu X Tu C Zhang X Electrochemical Deposition of Nanostructured Hydroxyapatite Coating on Titanium with Enhanced Early Stage Osteogenic Activity and Osseointegration
description	Minxun Lu,1,2 Hongjie Chen,2 Bo Yuan,2 Yong Zhou,1 Li Min,1 Zhanwen Xiao,2 Xiangdong Zhu,2 Chongqi Tu,1 Xingdong Zhang2 1Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, People’s Republic of China; 2National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, People’s Republic of ChinaCorrespondence: Xiangdong ZhuNational Engineering Research Center for Biomaterials, Sichuan University, No. 29 Wangjiang Road, Chengdu 610064, People’s Republic of ChinaTel +86-28-85470770Email zhu_xd1973@scu.edu.cnChongqi TuDepartment of Orthopedics, West China Hospital, Sichuan University, No. 37 Guoxue Street, Chengdu 610041, People’s Republic of ChinaTel +86-28-85422570Email Tuchongqi@yeah.netPurpose: The aim of research is to fabricate nanostructured hydroxyapatite (HA) coatings on the titanium via electrochemical deposition (ED). Additionally, the biological properties of the ED-produced HA (EDHA) coatings with a plate-like nanostructure were evaluated in vitro and in vivo by undertaking comparisons with those prepared by acid/alkali (AA) treatment and by plasma spray-produced HA (PSHA) nanotopography-free coatings.Materials and Methods: Nanoplate-like HA coatings were prepared through ED, and nanotopography-free PSHA coatings were fabricated. The surface morphology, phase composition, roughness, and wettability of these samples were investigated. Furthermore, the growth, proliferation, and osteogenic differentiation of MC3T3-E1 cells cultured on each sample were evaluated via in vitro experiments. Histological assessment and push-out tests for the bone–implant interface were performed to explore the effect of the EDHA coatings on the interfacial osseointegration in vivo.Results: XRD analysis showed that the strongest intensity for the EDHA coatings was at the (002) plane rather than at the regular (211) plane. Relatively higher surface roughness and greater wettability were observed for the EDHA coatings. Cellular experiments revealed that the plate-like nanostructured EDHA coatings not only possessed an ability, similar to that of PSHA coatings, to promote the adhesion and proliferation of MC3T3-E1 cells but also demonstrated significantly enhanced early or intermediate markers of osteogenic differentiation. Significant osseointegration enhancement in the early stage of implantation period and great bonding strength were observed at the interface of bone and EDHA samples. In comparison, relatively weak osseointegration and bonding strength of the bone–implant interface were observed for the AA treatment.Conclusion: The biological performance of the plate-like nanostructured EDHA coating, which was comparable with that of the PSHA, improves early-stage osteogenic differentiation and osseointegration abilities and has great potential for enhancing the initial stability and long-term survival of uncemented or 3D porous titanium implants.Keywords: electrochemical deposition, hydroxyapatite coatings, nanoscale, osteogenic activity, osseointegration
format	article
author	Lu M Chen H Yuan B Zhou Y Min L Xiao Z Zhu X Tu C Zhang X
author_facet	Lu M Chen H Yuan B Zhou Y Min L Xiao Z Zhu X Tu C Zhang X
author_sort	Lu M
title	Electrochemical Deposition of Nanostructured Hydroxyapatite Coating on Titanium with Enhanced Early Stage Osteogenic Activity and Osseointegration
title_short	Electrochemical Deposition of Nanostructured Hydroxyapatite Coating on Titanium with Enhanced Early Stage Osteogenic Activity and Osseointegration
title_full	Electrochemical Deposition of Nanostructured Hydroxyapatite Coating on Titanium with Enhanced Early Stage Osteogenic Activity and Osseointegration
title_fullStr	Electrochemical Deposition of Nanostructured Hydroxyapatite Coating on Titanium with Enhanced Early Stage Osteogenic Activity and Osseointegration
title_full_unstemmed	Electrochemical Deposition of Nanostructured Hydroxyapatite Coating on Titanium with Enhanced Early Stage Osteogenic Activity and Osseointegration
title_sort	electrochemical deposition of nanostructured hydroxyapatite coating on titanium with enhanced early stage osteogenic activity and osseointegration
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/ae1f119c681e4eecb778afd0d933f829
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Electrochemical Deposition of Nanostructured Hydroxyapatite Coating on Titanium with Enhanced Early Stage Osteogenic Activity and Osseointegration

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