P50, the small subunit of DNA polymerase delta, is required for mediation of the interaction of polymerase delta subassemblies with PCNA.
Mammalian DNA polymerase δ (pol δ), a four-subunit enzyme, plays a crucial and versatile role in DNA replication and various DNA repair processes. Its function as a chromosomal DNA polymerase is dependent on the association with proliferating cell nuclear antigen (PCNA) which functions as a molecula...
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oai:doaj.org-article:ae2234a9f8a3421ba80d4b5f31efda4a2021-11-18T07:35:08ZP50, the small subunit of DNA polymerase delta, is required for mediation of the interaction of polymerase delta subassemblies with PCNA.1932-620310.1371/journal.pone.0027092https://doaj.org/article/ae2234a9f8a3421ba80d4b5f31efda4a2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22073260/?tool=EBIhttps://doaj.org/toc/1932-6203Mammalian DNA polymerase δ (pol δ), a four-subunit enzyme, plays a crucial and versatile role in DNA replication and various DNA repair processes. Its function as a chromosomal DNA polymerase is dependent on the association with proliferating cell nuclear antigen (PCNA) which functions as a molecular sliding clamp. All four of the pol δ subunits (p125, p50, p68, and p12) have been reported to bind to PCNA. However, the identity of the subunit of pol δ that directly interacts with PCNA and is therefore primarily responsible for the processivity of the enzyme still remains controversial. Previous model for the network of protein-protein interactions of the pol δ-PCNA complex showed that pol δ might be able to interact with a single molecule of PCNA homotrimer through its three subunits, p125, p68, and p12 in which the p50 was not included in. Here, we have confirmed that the small subunit p50 of human pol δ truthfully interacts with PCNA by the use of far-Western analysis, quantitative ELISA assay, and subcellular co-localization. P50 is required for mediation of the interaction between pol δ subassemblies and PCNA homotrimer. Thus, pol δ interacts with PCNA via its four subunits.Yujue WangQian ZhangHuiqing ChenXiao LiWeijun MaiKeping ChenSufang ZhangErnest Y C LeeMarietta Y W T LeeYajing ZhouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27092 (2011) |
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Medicine R Science Q Yujue Wang Qian Zhang Huiqing Chen Xiao Li Weijun Mai Keping Chen Sufang Zhang Ernest Y C Lee Marietta Y W T Lee Yajing Zhou P50, the small subunit of DNA polymerase delta, is required for mediation of the interaction of polymerase delta subassemblies with PCNA. |
| description |
Mammalian DNA polymerase δ (pol δ), a four-subunit enzyme, plays a crucial and versatile role in DNA replication and various DNA repair processes. Its function as a chromosomal DNA polymerase is dependent on the association with proliferating cell nuclear antigen (PCNA) which functions as a molecular sliding clamp. All four of the pol δ subunits (p125, p50, p68, and p12) have been reported to bind to PCNA. However, the identity of the subunit of pol δ that directly interacts with PCNA and is therefore primarily responsible for the processivity of the enzyme still remains controversial. Previous model for the network of protein-protein interactions of the pol δ-PCNA complex showed that pol δ might be able to interact with a single molecule of PCNA homotrimer through its three subunits, p125, p68, and p12 in which the p50 was not included in. Here, we have confirmed that the small subunit p50 of human pol δ truthfully interacts with PCNA by the use of far-Western analysis, quantitative ELISA assay, and subcellular co-localization. P50 is required for mediation of the interaction between pol δ subassemblies and PCNA homotrimer. Thus, pol δ interacts with PCNA via its four subunits. |
| format |
article |
| author |
Yujue Wang Qian Zhang Huiqing Chen Xiao Li Weijun Mai Keping Chen Sufang Zhang Ernest Y C Lee Marietta Y W T Lee Yajing Zhou |
| author_facet |
Yujue Wang Qian Zhang Huiqing Chen Xiao Li Weijun Mai Keping Chen Sufang Zhang Ernest Y C Lee Marietta Y W T Lee Yajing Zhou |
| author_sort |
Yujue Wang |
| title |
P50, the small subunit of DNA polymerase delta, is required for mediation of the interaction of polymerase delta subassemblies with PCNA. |
| title_short |
P50, the small subunit of DNA polymerase delta, is required for mediation of the interaction of polymerase delta subassemblies with PCNA. |
| title_full |
P50, the small subunit of DNA polymerase delta, is required for mediation of the interaction of polymerase delta subassemblies with PCNA. |
| title_fullStr |
P50, the small subunit of DNA polymerase delta, is required for mediation of the interaction of polymerase delta subassemblies with PCNA. |
| title_full_unstemmed |
P50, the small subunit of DNA polymerase delta, is required for mediation of the interaction of polymerase delta subassemblies with PCNA. |
| title_sort |
p50, the small subunit of dna polymerase delta, is required for mediation of the interaction of polymerase delta subassemblies with pcna. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/ae2234a9f8a3421ba80d4b5f31efda4a |
| work_keys_str_mv |
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