Sustainable production of camptothecin from an Alternaria sp. isolated from Nothapodytes nimmoniana

Abstract Camptothecin the third most in demand alkaloid, is commercially extracted in India from the endangered plant, Nothapodytes nimmoniana. Endophytes, the microorganisms that reside within plants, are reported to have the ability to produce host–plant associated metabolites. Hence, our research...

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Autores principales:	I. A. H. Khwajah Mohinudeen, Rahul Kanumuri, K. N. Soujanya, R. Uma Shaanker, Suresh Kumar Rayala, Smita Srivastava
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Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ae22e4dd8720446297c476e7013c5b4d
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spelling	oai:doaj.org-article:ae22e4dd8720446297c476e7013c5b4d2021-12-02T14:01:34ZSustainable production of camptothecin from an Alternaria sp. isolated from Nothapodytes nimmoniana10.1038/s41598-020-79239-52045-2322https://doaj.org/article/ae22e4dd8720446297c476e7013c5b4d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79239-5https://doaj.org/toc/2045-2322Abstract Camptothecin the third most in demand alkaloid, is commercially extracted in India from the endangered plant, Nothapodytes nimmoniana. Endophytes, the microorganisms that reside within plants, are reported to have the ability to produce host–plant associated metabolites. Hence, our research aims to establish a sustainable and high camptothecin yielding endophyte, as an alternative source for commercial production of camptothecin. A total of 132 endophytic fungal strains were isolated from different plant parts (leaf, petiole, stem and bark) of N. nimmoniana, out of which 94 were found to produce camptothecin in suspension culture. Alternaria alstroemeriae (NCIM1408) and Alternaria burnsii (NCIM1409) demonstrated camptothecin yields up to 426.7 ± 33.6 µg/g DW and 403.3 ± 41.6 µg/g DW, respectively, the highest reported production to date. Unlike the reported product yield attenuation in endophytes with subculture in axenic state, Alternaria burnsii NCIM1409 could retain and sustain the production of camptothecin up to ~ 200 μg/g even after 12 continuous subculture cycles. The camptothecin biosynthesis in Alternaria burnsii NCIM1409 was confirmed using 13C carbon labelling (and cytotoxicity analysis on different cancer cell lines) and this strain can now be used to develop a sustainable bioprocess for in vitro production of camptothecin as an alternative to plant extraction.I. A. H. Khwajah MohinudeenRahul KanumuriK. N. SoujanyaR. Uma ShaankerSuresh Kumar RayalaSmita SrivastavaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q I. A. H. Khwajah Mohinudeen Rahul Kanumuri K. N. Soujanya R. Uma Shaanker Suresh Kumar Rayala Smita Srivastava Sustainable production of camptothecin from an Alternaria sp. isolated from Nothapodytes nimmoniana
description	Abstract Camptothecin the third most in demand alkaloid, is commercially extracted in India from the endangered plant, Nothapodytes nimmoniana. Endophytes, the microorganisms that reside within plants, are reported to have the ability to produce host–plant associated metabolites. Hence, our research aims to establish a sustainable and high camptothecin yielding endophyte, as an alternative source for commercial production of camptothecin. A total of 132 endophytic fungal strains were isolated from different plant parts (leaf, petiole, stem and bark) of N. nimmoniana, out of which 94 were found to produce camptothecin in suspension culture. Alternaria alstroemeriae (NCIM1408) and Alternaria burnsii (NCIM1409) demonstrated camptothecin yields up to 426.7 ± 33.6 µg/g DW and 403.3 ± 41.6 µg/g DW, respectively, the highest reported production to date. Unlike the reported product yield attenuation in endophytes with subculture in axenic state, Alternaria burnsii NCIM1409 could retain and sustain the production of camptothecin up to ~ 200 μg/g even after 12 continuous subculture cycles. The camptothecin biosynthesis in Alternaria burnsii NCIM1409 was confirmed using 13C carbon labelling (and cytotoxicity analysis on different cancer cell lines) and this strain can now be used to develop a sustainable bioprocess for in vitro production of camptothecin as an alternative to plant extraction.
format	article
author	I. A. H. Khwajah Mohinudeen Rahul Kanumuri K. N. Soujanya R. Uma Shaanker Suresh Kumar Rayala Smita Srivastava
author_facet	I. A. H. Khwajah Mohinudeen Rahul Kanumuri K. N. Soujanya R. Uma Shaanker Suresh Kumar Rayala Smita Srivastava
author_sort	I. A. H. Khwajah Mohinudeen
title	Sustainable production of camptothecin from an Alternaria sp. isolated from Nothapodytes nimmoniana
title_short	Sustainable production of camptothecin from an Alternaria sp. isolated from Nothapodytes nimmoniana
title_full	Sustainable production of camptothecin from an Alternaria sp. isolated from Nothapodytes nimmoniana
title_fullStr	Sustainable production of camptothecin from an Alternaria sp. isolated from Nothapodytes nimmoniana
title_full_unstemmed	Sustainable production of camptothecin from an Alternaria sp. isolated from Nothapodytes nimmoniana
title_sort	sustainable production of camptothecin from an alternaria sp. isolated from nothapodytes nimmoniana
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ae22e4dd8720446297c476e7013c5b4d
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Sustainable production of camptothecin from an Alternaria sp. isolated from Nothapodytes nimmoniana

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