Sizing Up the Uncultured Microbial Majority

ABSTRACT Predicting the total number of microbial cells on Earth and exploring the full diversity of life are fundamental research concepts that have undergone paradigm shifts in the genomic era. In this issue, Lloyd and colleagues (K. G. Lloyd, A. D. Steen, J. L. Ladau, J. Yin, and L. Crosby, mSyst...

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Autor principal:	Laura A. Hug
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	amplicon sequencing metagenomics metatranscriptomics microbial diversity Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ae24f000fdfb49d5b9b4d21248e9f0ab
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spelling	oai:doaj.org-article:ae24f000fdfb49d5b9b4d21248e9f0ab2021-12-02T19:46:18ZSizing Up the Uncultured Microbial Majority10.1128/mSystems.00185-182379-5077https://doaj.org/article/ae24f000fdfb49d5b9b4d21248e9f0ab2018-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00185-18https://doaj.org/toc/2379-5077ABSTRACT Predicting the total number of microbial cells on Earth and exploring the full diversity of life are fundamental research concepts that have undergone paradigm shifts in the genomic era. In this issue, Lloyd and colleagues (K. G. Lloyd, A. D. Steen, J. L. Ladau, J. Yin, and L. Crosby, mSystems 3:e00055-18, https://doi.org/10.1128/mSystems.00055-18, 2018) present results that combine these two concepts by estimating the total diversity of all cells from Earth’s environments. Leveraging publicly available amplicon, metagenomic, and metatranscriptomic datasets, they determined that nearly all environments are dominated by uncultured lineages, with the exception of humans and human-associated habitats. They define a new concept: phylogenetically diverse noncultured cells (PDNC). Unlike viable but nonculturable cells (VBNC), PDNC are microorganisms for which traditional isolation techniques may never succeed. Lloyd et al. estimate that the majority of microorganisms in Earth’s ecosystems may be PDNC and conclude that culture-independent methods combined with innovative culturing techniques may be required to understand the ecology and physiology of these abundant and divergent microorganisms.Laura A. HugAmerican Society for Microbiologyarticleamplicon sequencingmetagenomicsmetatranscriptomicsmicrobial diversityMicrobiologyQR1-502ENmSystems, Vol 3, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	amplicon sequencing metagenomics metatranscriptomics microbial diversity Microbiology QR1-502
spellingShingle	amplicon sequencing metagenomics metatranscriptomics microbial diversity Microbiology QR1-502 Laura A. Hug Sizing Up the Uncultured Microbial Majority
description	ABSTRACT Predicting the total number of microbial cells on Earth and exploring the full diversity of life are fundamental research concepts that have undergone paradigm shifts in the genomic era. In this issue, Lloyd and colleagues (K. G. Lloyd, A. D. Steen, J. L. Ladau, J. Yin, and L. Crosby, mSystems 3:e00055-18, https://doi.org/10.1128/mSystems.00055-18, 2018) present results that combine these two concepts by estimating the total diversity of all cells from Earth’s environments. Leveraging publicly available amplicon, metagenomic, and metatranscriptomic datasets, they determined that nearly all environments are dominated by uncultured lineages, with the exception of humans and human-associated habitats. They define a new concept: phylogenetically diverse noncultured cells (PDNC). Unlike viable but nonculturable cells (VBNC), PDNC are microorganisms for which traditional isolation techniques may never succeed. Lloyd et al. estimate that the majority of microorganisms in Earth’s ecosystems may be PDNC and conclude that culture-independent methods combined with innovative culturing techniques may be required to understand the ecology and physiology of these abundant and divergent microorganisms.
format	article
author	Laura A. Hug
author_facet	Laura A. Hug
author_sort	Laura A. Hug
title	Sizing Up the Uncultured Microbial Majority
title_short	Sizing Up the Uncultured Microbial Majority
title_full	Sizing Up the Uncultured Microbial Majority
title_fullStr	Sizing Up the Uncultured Microbial Majority
title_full_unstemmed	Sizing Up the Uncultured Microbial Majority
title_sort	sizing up the uncultured microbial majority
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/ae24f000fdfb49d5b9b4d21248e9f0ab
work_keys_str_mv	AT lauraahug sizinguptheunculturedmicrobialmajority
_version_	1718376045590085632

Sizing Up the Uncultured Microbial Majority

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