Neutrophil extracellular traps induce a hypercoagulable state in glioma

Abstract Background Venous thromboembolism (VTE) is one of the leading complications in glioma patients. Neutrophil extracellular traps (NETs) have been reported to play a critical role in the physiopathology of cancer. We aimed to investigate the presence and potential role of NETs in the hypercoag...

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Autores principales:	Shihua Zhang, Mengfan Guo, Qianzi Liu, Jingfeng Liu, Yankun Cui
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	endothelial cells glioma neutrophil extracellular traps platelet venous thromboembolism Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ae32652e8b5b45f78041b70ad19a5eb7
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spelling	oai:doaj.org-article:ae32652e8b5b45f78041b70ad19a5eb72021-11-12T19:57:14ZNeutrophil extracellular traps induce a hypercoagulable state in glioma2050-452710.1002/iid3.488https://doaj.org/article/ae32652e8b5b45f78041b70ad19a5eb72021-12-01T00:00:00Zhttps://doi.org/10.1002/iid3.488https://doaj.org/toc/2050-4527Abstract Background Venous thromboembolism (VTE) is one of the leading complications in glioma patients. Neutrophil extracellular traps (NETs) have been reported to play a critical role in the physiopathology of cancer. We aimed to investigate the presence and potential role of NETs in the hypercoagulable state in glioma patients. Moreover, we evaluated the interaction between NETs and endothelial cells (ECs) in glioma patients. Methods The plasma levels of NETs were detected by enzyme‐linked immunosorbent assay. The NET procoagulant activity was performed based on fibrin formation assays. The NET generation and NET‐treated ECs in vitro were observed by confocal microscopy. Activated platelets (PLTs) and PLT‐neutrophil aggregates were detected by flow cytometry. Results Plasma NET markers were significantly higher in stage III/IV glioma patients than in stage I/II glioma patients and healthy subjects. PLTs from glioma patients tended to induce NET formation than those from healthy subjects. NETs contributed to the hypercoagulable state in glioma patients. After ECs were incubated with NETs isolated from stage III/IV glioma patients, they lost their intercellular connections and were converted into procoagulant phenotypes. Combining DNase I and activated protein C markedly decreased endothelial dysfunction. Conclusions Our results showed the interaction between NETs and hypercoagulability in glioma patients. Targeting NETs may be a potential therapeutic and prevention direction for thrombotic complications in glioma patients.Shihua ZhangMengfan GuoQianzi LiuJingfeng LiuYankun CuiWileyarticleendothelial cellsgliomaneutrophil extracellular trapsplateletvenous thromboembolismImmunologic diseases. AllergyRC581-607ENImmunity, Inflammation and Disease, Vol 9, Iss 4, Pp 1383-1393 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endothelial cells glioma neutrophil extracellular traps platelet venous thromboembolism Immunologic diseases. Allergy RC581-607
spellingShingle	endothelial cells glioma neutrophil extracellular traps platelet venous thromboembolism Immunologic diseases. Allergy RC581-607 Shihua Zhang Mengfan Guo Qianzi Liu Jingfeng Liu Yankun Cui Neutrophil extracellular traps induce a hypercoagulable state in glioma
description	Abstract Background Venous thromboembolism (VTE) is one of the leading complications in glioma patients. Neutrophil extracellular traps (NETs) have been reported to play a critical role in the physiopathology of cancer. We aimed to investigate the presence and potential role of NETs in the hypercoagulable state in glioma patients. Moreover, we evaluated the interaction between NETs and endothelial cells (ECs) in glioma patients. Methods The plasma levels of NETs were detected by enzyme‐linked immunosorbent assay. The NET procoagulant activity was performed based on fibrin formation assays. The NET generation and NET‐treated ECs in vitro were observed by confocal microscopy. Activated platelets (PLTs) and PLT‐neutrophil aggregates were detected by flow cytometry. Results Plasma NET markers were significantly higher in stage III/IV glioma patients than in stage I/II glioma patients and healthy subjects. PLTs from glioma patients tended to induce NET formation than those from healthy subjects. NETs contributed to the hypercoagulable state in glioma patients. After ECs were incubated with NETs isolated from stage III/IV glioma patients, they lost their intercellular connections and were converted into procoagulant phenotypes. Combining DNase I and activated protein C markedly decreased endothelial dysfunction. Conclusions Our results showed the interaction between NETs and hypercoagulability in glioma patients. Targeting NETs may be a potential therapeutic and prevention direction for thrombotic complications in glioma patients.
format	article
author	Shihua Zhang Mengfan Guo Qianzi Liu Jingfeng Liu Yankun Cui
author_facet	Shihua Zhang Mengfan Guo Qianzi Liu Jingfeng Liu Yankun Cui
author_sort	Shihua Zhang
title	Neutrophil extracellular traps induce a hypercoagulable state in glioma
title_short	Neutrophil extracellular traps induce a hypercoagulable state in glioma
title_full	Neutrophil extracellular traps induce a hypercoagulable state in glioma
title_fullStr	Neutrophil extracellular traps induce a hypercoagulable state in glioma
title_full_unstemmed	Neutrophil extracellular traps induce a hypercoagulable state in glioma
title_sort	neutrophil extracellular traps induce a hypercoagulable state in glioma
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/ae32652e8b5b45f78041b70ad19a5eb7
work_keys_str_mv	AT shihuazhang neutrophilextracellulartrapsinduceahypercoagulablestateinglioma AT mengfanguo neutrophilextracellulartrapsinduceahypercoagulablestateinglioma AT qianziliu neutrophilextracellulartrapsinduceahypercoagulablestateinglioma AT jingfengliu neutrophilextracellulartrapsinduceahypercoagulablestateinglioma AT yankuncui neutrophilextracellulartrapsinduceahypercoagulablestateinglioma
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Neutrophil extracellular traps induce a hypercoagulable state in glioma

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