The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling
Abstract Genome-scale metabolic network models can be used for various analyses including the prediction of metabolic responses to changes in the environment. Legumes are well known for their rhizobial symbiosis that introduces nitrogen into the global nutrient cycle. Here, we describe a fully compa...
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Nature Portfolio
2018
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oai:doaj.org-article:ae34a0ff7f404a1687dc54d12640b4202021-12-02T15:08:17ZThe intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling10.1038/s41598-018-30884-x2045-2322https://doaj.org/article/ae34a0ff7f404a1687dc54d12640b4202018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30884-xhttps://doaj.org/toc/2045-2322Abstract Genome-scale metabolic network models can be used for various analyses including the prediction of metabolic responses to changes in the environment. Legumes are well known for their rhizobial symbiosis that introduces nitrogen into the global nutrient cycle. Here, we describe a fully compartmentalised, mass and charge-balanced, genome-scale model of the clover Medicago truncatula, which has been adopted as a model organism for legumes. We employed flux balance analysis to demonstrate that the network is capable of producing biomass components in experimentally observed proportions, during day and night. By connecting the plant model to a model of its rhizobial symbiont, Sinorhizobium meliloti, we were able to investigate the effects of the symbiosis on metabolic fluxes and plant growth and could demonstrate how oxygen availability influences metabolic exchanges between plant and symbiont, thus elucidating potential benefits of inter organism amino acid cycling. We thus provide a modelling framework, in which the interlinked metabolism of plants and nodules can be studied from a theoretical perspective.Thomas PfauNils ChristianShyam K. MasakapalliLee J. SweetloveMark G. PoolmanOliver EbenhöhNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
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DOAJ |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Thomas Pfau Nils Christian Shyam K. Masakapalli Lee J. Sweetlove Mark G. Poolman Oliver Ebenhöh The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling |
| description |
Abstract Genome-scale metabolic network models can be used for various analyses including the prediction of metabolic responses to changes in the environment. Legumes are well known for their rhizobial symbiosis that introduces nitrogen into the global nutrient cycle. Here, we describe a fully compartmentalised, mass and charge-balanced, genome-scale model of the clover Medicago truncatula, which has been adopted as a model organism for legumes. We employed flux balance analysis to demonstrate that the network is capable of producing biomass components in experimentally observed proportions, during day and night. By connecting the plant model to a model of its rhizobial symbiont, Sinorhizobium meliloti, we were able to investigate the effects of the symbiosis on metabolic fluxes and plant growth and could demonstrate how oxygen availability influences metabolic exchanges between plant and symbiont, thus elucidating potential benefits of inter organism amino acid cycling. We thus provide a modelling framework, in which the interlinked metabolism of plants and nodules can be studied from a theoretical perspective. |
| format |
article |
| author |
Thomas Pfau Nils Christian Shyam K. Masakapalli Lee J. Sweetlove Mark G. Poolman Oliver Ebenhöh |
| author_facet |
Thomas Pfau Nils Christian Shyam K. Masakapalli Lee J. Sweetlove Mark G. Poolman Oliver Ebenhöh |
| author_sort |
Thomas Pfau |
| title |
The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling |
| title_short |
The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling |
| title_full |
The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling |
| title_fullStr |
The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling |
| title_full_unstemmed |
The intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling |
| title_sort |
intertwined metabolism during symbiotic nitrogen fixation elucidated by metabolic modelling |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/ae34a0ff7f404a1687dc54d12640b420 |
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