Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra

The sea cucumber Holothuria scabra is both an economically important species in Asian countries and an emerging experimental model for research studies in regeneration and medicinal bioactives. Growth factors and their receptors are known to be key components that guide tissue repair and renewal, ye...

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Autores principales:	Napamanee Kornthong, Teva Phanaksri, Jirawat Saetan, Supawadee Duangprom, Buranee Lekskul, Tipok Vivattanasarn, Sineenart Songkoomkrong, Prapaporn Jattujan, Scott F. Cummins, Prasert Sobhon, Saowaros Suwansa-ard
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Growth factors Sea cucumber Holothuria scabra Gene expression Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/ae355fa0af3443e6bd6e939a70f6d922
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spelling	oai:doaj.org-article:ae355fa0af3443e6bd6e939a70f6d9222021-12-02T05:02:53ZIdentification and localization of growth factor genes in the sea cucumber, Holothuria scabra2405-844010.1016/j.heliyon.2021.e08370https://doaj.org/article/ae355fa0af3443e6bd6e939a70f6d9222021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021024737https://doaj.org/toc/2405-8440The sea cucumber Holothuria scabra is both an economically important species in Asian countries and an emerging experimental model for research studies in regeneration and medicinal bioactives. Growth factors and their receptors are known to be key components that guide tissue repair and renewal, yet validation of their presence in H. scabra has not been established. We performed a targeted in silico search of H. scabra transcriptome data to elucidate conserved growth factor family and receptor genes. In total, 42 transcripts were identified, of which 9 were validated by gene cloning and sequencing. The H. scabra growth factor genes, such as bone morphogenetic protein 2A (BMP 2A), bone morphogenetic protein 5-like (BMP5-like), neurotrophin (NT) and fibroblast growth factor 18 (FGF18), were selected for further analyses, including phylogenetic comparison and spatial gene expression using RT-PCR and in situ hybridization. Expression of all genes investigated were widespread in multiple tissues. However, BMP 2A, BMP5-like and NT were found extensively in the radial nerve cord cells, while FGF18 was highly expressed in connective tissue layer of the body wall. Our identification and expression analysis of the H. scabra growth factor genes provided the molecular information of growth factors in this species which may ultimately complement the research in regenerative medicine.Napamanee KornthongTeva PhanaksriJirawat SaetanSupawadee DuangpromBuranee LekskulTipok VivattanasarnSineenart SongkoomkrongPrapaporn JattujanScott F. CumminsPrasert SobhonSaowaros Suwansa-ardElsevierarticleGrowth factorsSea cucumberHolothuria scabraGene expressionScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08370- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Growth factors Sea cucumber Holothuria scabra Gene expression Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Growth factors Sea cucumber Holothuria scabra Gene expression Science (General) Q1-390 Social sciences (General) H1-99 Napamanee Kornthong Teva Phanaksri Jirawat Saetan Supawadee Duangprom Buranee Lekskul Tipok Vivattanasarn Sineenart Songkoomkrong Prapaporn Jattujan Scott F. Cummins Prasert Sobhon Saowaros Suwansa-ard Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra
description	The sea cucumber Holothuria scabra is both an economically important species in Asian countries and an emerging experimental model for research studies in regeneration and medicinal bioactives. Growth factors and their receptors are known to be key components that guide tissue repair and renewal, yet validation of their presence in H. scabra has not been established. We performed a targeted in silico search of H. scabra transcriptome data to elucidate conserved growth factor family and receptor genes. In total, 42 transcripts were identified, of which 9 were validated by gene cloning and sequencing. The H. scabra growth factor genes, such as bone morphogenetic protein 2A (BMP 2A), bone morphogenetic protein 5-like (BMP5-like), neurotrophin (NT) and fibroblast growth factor 18 (FGF18), were selected for further analyses, including phylogenetic comparison and spatial gene expression using RT-PCR and in situ hybridization. Expression of all genes investigated were widespread in multiple tissues. However, BMP 2A, BMP5-like and NT were found extensively in the radial nerve cord cells, while FGF18 was highly expressed in connective tissue layer of the body wall. Our identification and expression analysis of the H. scabra growth factor genes provided the molecular information of growth factors in this species which may ultimately complement the research in regenerative medicine.
format	article
author	Napamanee Kornthong Teva Phanaksri Jirawat Saetan Supawadee Duangprom Buranee Lekskul Tipok Vivattanasarn Sineenart Songkoomkrong Prapaporn Jattujan Scott F. Cummins Prasert Sobhon Saowaros Suwansa-ard
author_facet	Napamanee Kornthong Teva Phanaksri Jirawat Saetan Supawadee Duangprom Buranee Lekskul Tipok Vivattanasarn Sineenart Songkoomkrong Prapaporn Jattujan Scott F. Cummins Prasert Sobhon Saowaros Suwansa-ard
author_sort	Napamanee Kornthong
title	Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra
title_short	Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra
title_full	Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra
title_fullStr	Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra
title_full_unstemmed	Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra
title_sort	identification and localization of growth factor genes in the sea cucumber, holothuria scabra
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/ae355fa0af3443e6bd6e939a70f6d922
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Identification and localization of growth factor genes in the sea cucumber, Holothuria scabra

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