Anticancer properties of peptide fragments of hair proteins.

The primary function of hair and fur covering mammalian skin is to provide mechanical and thermal protection for the body. The proteins that constitute hair are extremely resistant to degradation by environmental factors. However, even durable materials can be slowly broken down by mechanical stress...

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Autores principales:	Sergiusz Markowicz, Joanna Matalinska, Katarzyna Kurzepa, Marta Bochynska, Marzena Biernacka, Anna Samluk, Dorota Dudek, Henryk Skurzak, Masaaki Yoshikawa, Andrzej W Lipkowski
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ae38e2aa526c416bb14dab47a051200d
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spelling	oai:doaj.org-article:ae38e2aa526c416bb14dab47a051200d2021-11-18T08:16:20ZAnticancer properties of peptide fragments of hair proteins.1932-620310.1371/journal.pone.0098073https://doaj.org/article/ae38e2aa526c416bb14dab47a051200d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24915193/?tool=EBIhttps://doaj.org/toc/1932-6203The primary function of hair and fur covering mammalian skin is to provide mechanical and thermal protection for the body. The proteins that constitute hair are extremely resistant to degradation by environmental factors. However, even durable materials can be slowly broken down by mechanical stresses, biodegradation mediated by endogenous enzymes in the skin or host microbes. We hypothesised that the biodegradation products of hair may possess bioprotective properties, which supplement their physical protective properties. Although evolutionary processes have led to a reduction in the amount of hair on the human body, it is possible that the bioprotective properties of hair biodegradation products have persisted. The human skin is exposed to various environmental carcinogenic factors. Therefore, we hypothesised that the potential bioprotective mechanisms of hair degradation products affect melanoma growth. We used pepsin to partially digest hair enzymatically, and this process produced a water-soluble lysate containing a mixture of peptides, including fragments of keratin and keratin-associated proteins. We found out that the mixtures of soluble peptides obtained from human hair inhibited the proliferation of human melanoma cells in vitro. Moreover, the hair-derived peptide mixtures also inhibited the proliferation of B lymphoma cells and urinary bladder cancer cells. Normal human cells varied in their susceptibility to the effects of the lysate; the hair-derived peptide mixtures modulated the proliferation of normal human fibroblasts but did not inhibit the proliferation of human mesenchymal cells derived from umbilical cord stromal cells. These results suggest that hair-derived peptides may represent a new class of anti-proliferative factors derived from basically structural proteins. Identification of active regulatory compounds and recognition of the mechanism of their action might pave the way to elaboration of new anticancer drugs.Sergiusz MarkowiczJoanna MatalinskaKatarzyna KurzepaMarta BochynskaMarzena BiernackaAnna SamlukDorota DudekHenryk SkurzakMasaaki YoshikawaAndrzej W LipkowskiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98073 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sergiusz Markowicz Joanna Matalinska Katarzyna Kurzepa Marta Bochynska Marzena Biernacka Anna Samluk Dorota Dudek Henryk Skurzak Masaaki Yoshikawa Andrzej W Lipkowski Anticancer properties of peptide fragments of hair proteins.
description	The primary function of hair and fur covering mammalian skin is to provide mechanical and thermal protection for the body. The proteins that constitute hair are extremely resistant to degradation by environmental factors. However, even durable materials can be slowly broken down by mechanical stresses, biodegradation mediated by endogenous enzymes in the skin or host microbes. We hypothesised that the biodegradation products of hair may possess bioprotective properties, which supplement their physical protective properties. Although evolutionary processes have led to a reduction in the amount of hair on the human body, it is possible that the bioprotective properties of hair biodegradation products have persisted. The human skin is exposed to various environmental carcinogenic factors. Therefore, we hypothesised that the potential bioprotective mechanisms of hair degradation products affect melanoma growth. We used pepsin to partially digest hair enzymatically, and this process produced a water-soluble lysate containing a mixture of peptides, including fragments of keratin and keratin-associated proteins. We found out that the mixtures of soluble peptides obtained from human hair inhibited the proliferation of human melanoma cells in vitro. Moreover, the hair-derived peptide mixtures also inhibited the proliferation of B lymphoma cells and urinary bladder cancer cells. Normal human cells varied in their susceptibility to the effects of the lysate; the hair-derived peptide mixtures modulated the proliferation of normal human fibroblasts but did not inhibit the proliferation of human mesenchymal cells derived from umbilical cord stromal cells. These results suggest that hair-derived peptides may represent a new class of anti-proliferative factors derived from basically structural proteins. Identification of active regulatory compounds and recognition of the mechanism of their action might pave the way to elaboration of new anticancer drugs.
format	article
author	Sergiusz Markowicz Joanna Matalinska Katarzyna Kurzepa Marta Bochynska Marzena Biernacka Anna Samluk Dorota Dudek Henryk Skurzak Masaaki Yoshikawa Andrzej W Lipkowski
author_facet	Sergiusz Markowicz Joanna Matalinska Katarzyna Kurzepa Marta Bochynska Marzena Biernacka Anna Samluk Dorota Dudek Henryk Skurzak Masaaki Yoshikawa Andrzej W Lipkowski
author_sort	Sergiusz Markowicz
title	Anticancer properties of peptide fragments of hair proteins.
title_short	Anticancer properties of peptide fragments of hair proteins.
title_full	Anticancer properties of peptide fragments of hair proteins.
title_fullStr	Anticancer properties of peptide fragments of hair proteins.
title_full_unstemmed	Anticancer properties of peptide fragments of hair proteins.
title_sort	anticancer properties of peptide fragments of hair proteins.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ae38e2aa526c416bb14dab47a051200d
work_keys_str_mv	AT sergiuszmarkowicz anticancerpropertiesofpeptidefragmentsofhairproteins AT joannamatalinska anticancerpropertiesofpeptidefragmentsofhairproteins AT katarzynakurzepa anticancerpropertiesofpeptidefragmentsofhairproteins AT martabochynska anticancerpropertiesofpeptidefragmentsofhairproteins AT marzenabiernacka anticancerpropertiesofpeptidefragmentsofhairproteins AT annasamluk anticancerpropertiesofpeptidefragmentsofhairproteins AT dorotadudek anticancerpropertiesofpeptidefragmentsofhairproteins AT henrykskurzak anticancerpropertiesofpeptidefragmentsofhairproteins AT masaakiyoshikawa anticancerpropertiesofpeptidefragmentsofhairproteins AT andrzejwlipkowski anticancerpropertiesofpeptidefragmentsofhairproteins
_version_	1718422004612202496

Anticancer properties of peptide fragments of hair proteins.

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