Arp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCR+ endosome trafficking

Abstract T cell receptor (TCR) signaling is important for T cell homeostasis and function. However, how surface TCR levels are regulated and its biological significance on T cells remains largely unknown. Here, we show that the T cell-specific deletion of Arpc2, a component of Arp2/3 complex, result...

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Autores principales:	Ye Zhang, Hao Shen, Haifeng Liu, Haiyun Feng, Yan Liu, Xiaoyan Zhu, Xiaolong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ae3bc8bd6ab84d138b7f827da4167c83
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spelling	oai:doaj.org-article:ae3bc8bd6ab84d138b7f827da4167c832021-12-02T16:06:01ZArp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCR+ endosome trafficking10.1038/s41598-017-08357-42045-2322https://doaj.org/article/ae3bc8bd6ab84d138b7f827da4167c832017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08357-4https://doaj.org/toc/2045-2322Abstract T cell receptor (TCR) signaling is important for T cell homeostasis and function. However, how surface TCR levels are regulated and its biological significance on T cells remains largely unknown. Here, we show that the T cell-specific deletion of Arpc2, a component of Arp2/3 complex, results in compromised peripheral T cell homeostasis. Arp2/3 complex-nucleated actin filaments are essential for maintaining surface TCR levels by regulating TCR+ endosome trafficking in resting state and controlling polarization of TCR+ endosomes during immune synapse formation in T cells. Additionally, Arpc2-TKO T cells are unable to form immune synapse. Interestingly, defected T cell homeostasis is caused by reduced surface TCR levels but not impaired immune synapse formation. Collectively, our findings suggest that Arp2/3 complex-nucleated actin filaments are required for maintaining surface TCR levels via regulating TCR+ endosome trafficking which is essential for T cell homeostasis.Ye ZhangHao ShenHaifeng LiuHaiyun FengYan LiuXiaoyan ZhuXiaolong LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ye Zhang Hao Shen Haifeng Liu Haiyun Feng Yan Liu Xiaoyan Zhu Xiaolong Liu Arp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCR+ endosome trafficking
description	Abstract T cell receptor (TCR) signaling is important for T cell homeostasis and function. However, how surface TCR levels are regulated and its biological significance on T cells remains largely unknown. Here, we show that the T cell-specific deletion of Arpc2, a component of Arp2/3 complex, results in compromised peripheral T cell homeostasis. Arp2/3 complex-nucleated actin filaments are essential for maintaining surface TCR levels by regulating TCR+ endosome trafficking in resting state and controlling polarization of TCR+ endosomes during immune synapse formation in T cells. Additionally, Arpc2-TKO T cells are unable to form immune synapse. Interestingly, defected T cell homeostasis is caused by reduced surface TCR levels but not impaired immune synapse formation. Collectively, our findings suggest that Arp2/3 complex-nucleated actin filaments are required for maintaining surface TCR levels via regulating TCR+ endosome trafficking which is essential for T cell homeostasis.
format	article
author	Ye Zhang Hao Shen Haifeng Liu Haiyun Feng Yan Liu Xiaoyan Zhu Xiaolong Liu
author_facet	Ye Zhang Hao Shen Haifeng Liu Haiyun Feng Yan Liu Xiaoyan Zhu Xiaolong Liu
author_sort	Ye Zhang
title	Arp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCR+ endosome trafficking
title_short	Arp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCR+ endosome trafficking
title_full	Arp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCR+ endosome trafficking
title_fullStr	Arp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCR+ endosome trafficking
title_full_unstemmed	Arp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCR+ endosome trafficking
title_sort	arp2/3 complex controls t cell homeostasis by maintaining surface tcr levels via regulating tcr+ endosome trafficking
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ae3bc8bd6ab84d138b7f827da4167c83
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Arp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCR+ endosome trafficking

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