Pterostilbene Inhibits the Melanogenesis Activity in UVB-Irradiated B164A5 Cells

Pterostilbene is a potent antioxidant and anti-inflammatory agent. However, its chemopreventive effects via anti-tyrosinase activity and inhibitory effects on melanin content have not been reported previously. Hence, this study aimed to investigate the anti-melanogenic activity of pterostilbene on U...

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Main Authors:	Dayang Fredalina Basri, Leong Chen Lew, Raveena Vaidheswary Muralitharan, Tava Shelan Nagapan, Ahmad Rohi Ghazali
Format:	article
Language:	EN
Published:	SAGE Publishing 2021
Subjects:	Therapeutics. Pharmacology RM1-950
Online Access:	https://doaj.org/article/ae42d3d9ad734a0faee19f213304e897
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spelling	oai:doaj.org-article:ae42d3d9ad734a0faee19f213304e8972021-11-18T23:33:45ZPterostilbene Inhibits the Melanogenesis Activity in UVB-Irradiated B164A5 Cells1559-325810.1177/15593258211047651https://doaj.org/article/ae42d3d9ad734a0faee19f213304e8972021-10-01T00:00:00Zhttps://doi.org/10.1177/15593258211047651https://doaj.org/toc/1559-3258Pterostilbene is a potent antioxidant and anti-inflammatory agent. However, its chemopreventive effects via anti-tyrosinase activity and inhibitory effects on melanin content have not been reported previously. Hence, this study aimed to investigate the anti-melanogenic activity of pterostilbene on UVB-irradiated B164A5 mouse melanoma cells. The effects of pterostilbene and resveratrol on cell viability were determined by MTT assay, whereas melanin content and tyrosinase assay were employed to assess melanogenesis activity. Western blot analysis was performed to determine the tyrosinase expression. Based on the MTT assay, the IC 50 value of pterostilbene on UVB-irradiated B164A5 cells was 34.0 ± 3.43 μM, in comparison to resveratrol (>100 μM). Next, 5 and 10 μM pterostilbene showed a significant dose-dependent inhibition ( P < .01) of tyrosinase activity in UVB-irradiated B164A5 cells at 37.14 ± 2.71% and 58.36 ± 6.8%, respectively. The findings from the tyrosinase assay also confirmed the downregulation of tyrosinase expression in UVB-irradiated B164A5 cells as measured by Western blot analysis. Finally, 10 μM pterostilbene showed a significantly decreased melanin content ( P < .01) in UVB-irradiated B164A5 cells, at 27.34 ± .98 μg/mL. In conclusion, pterostilbene showed anti-melanogenic activity that was 10 times more potent than resveratrol in the UVB-irradiated B164A5 cell.Dayang Fredalina BasriLeong Chen LewRaveena Vaidheswary MuralitharanTava Shelan NagapanAhmad Rohi GhazaliSAGE PublishingarticleTherapeutics. PharmacologyRM1-950ENDose-Response, Vol 19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Therapeutics. Pharmacology RM1-950
spellingShingle	Therapeutics. Pharmacology RM1-950 Dayang Fredalina Basri Leong Chen Lew Raveena Vaidheswary Muralitharan Tava Shelan Nagapan Ahmad Rohi Ghazali Pterostilbene Inhibits the Melanogenesis Activity in UVB-Irradiated B164A5 Cells
description	Pterostilbene is a potent antioxidant and anti-inflammatory agent. However, its chemopreventive effects via anti-tyrosinase activity and inhibitory effects on melanin content have not been reported previously. Hence, this study aimed to investigate the anti-melanogenic activity of pterostilbene on UVB-irradiated B164A5 mouse melanoma cells. The effects of pterostilbene and resveratrol on cell viability were determined by MTT assay, whereas melanin content and tyrosinase assay were employed to assess melanogenesis activity. Western blot analysis was performed to determine the tyrosinase expression. Based on the MTT assay, the IC 50 value of pterostilbene on UVB-irradiated B164A5 cells was 34.0 ± 3.43 μM, in comparison to resveratrol (>100 μM). Next, 5 and 10 μM pterostilbene showed a significant dose-dependent inhibition ( P < .01) of tyrosinase activity in UVB-irradiated B164A5 cells at 37.14 ± 2.71% and 58.36 ± 6.8%, respectively. The findings from the tyrosinase assay also confirmed the downregulation of tyrosinase expression in UVB-irradiated B164A5 cells as measured by Western blot analysis. Finally, 10 μM pterostilbene showed a significantly decreased melanin content ( P < .01) in UVB-irradiated B164A5 cells, at 27.34 ± .98 μg/mL. In conclusion, pterostilbene showed anti-melanogenic activity that was 10 times more potent than resveratrol in the UVB-irradiated B164A5 cell.
format	article
author	Dayang Fredalina Basri Leong Chen Lew Raveena Vaidheswary Muralitharan Tava Shelan Nagapan Ahmad Rohi Ghazali
author_facet	Dayang Fredalina Basri Leong Chen Lew Raveena Vaidheswary Muralitharan Tava Shelan Nagapan Ahmad Rohi Ghazali
author_sort	Dayang Fredalina Basri
title	Pterostilbene Inhibits the Melanogenesis Activity in UVB-Irradiated B164A5 Cells
title_short	Pterostilbene Inhibits the Melanogenesis Activity in UVB-Irradiated B164A5 Cells
title_full	Pterostilbene Inhibits the Melanogenesis Activity in UVB-Irradiated B164A5 Cells
title_fullStr	Pterostilbene Inhibits the Melanogenesis Activity in UVB-Irradiated B164A5 Cells
title_full_unstemmed	Pterostilbene Inhibits the Melanogenesis Activity in UVB-Irradiated B164A5 Cells
title_sort	pterostilbene inhibits the melanogenesis activity in uvb-irradiated b164a5 cells
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/ae42d3d9ad734a0faee19f213304e897
work_keys_str_mv	AT dayangfredalinabasri pterostilbeneinhibitsthemelanogenesisactivityinuvbirradiatedb164a5cells AT leongchenlew pterostilbeneinhibitsthemelanogenesisactivityinuvbirradiatedb164a5cells AT raveenavaidheswarymuralitharan pterostilbeneinhibitsthemelanogenesisactivityinuvbirradiatedb164a5cells AT tavashelannagapan pterostilbeneinhibitsthemelanogenesisactivityinuvbirradiatedb164a5cells AT ahmadrohighazali pterostilbeneinhibitsthemelanogenesisactivityinuvbirradiatedb164a5cells
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Pterostilbene Inhibits the Melanogenesis Activity in UVB-Irradiated B164A5 Cells

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